Immunohistochemical localization of progesterone receptors alpha (PRA) in ovary of the pseudopregnant rabbit

Abstract

Progesterone plays an important role in the reproductive function and follicular development in mammals. The aim of the present study was to examine the localization of progesterone receptor alpha (PRA) in ovary of pseudopregnant rabbit by immunohistochemical methods. Samples were collected from 14 h. to 18 days of pseudopregnancy. At the first stage of pseudopregnancy (14 h.), the rabbit ovary showed moderate immunostaining of PRA in the granulosa cells and theca interna cells of preovulatory follicle and in the stroma cells. At the middle stage of pseudopregnancy (3-7 days), the rabbit ovary showed strong immunostaining of PRA in ovarian surface epithelial cells, follicular cells of the primary follicle, granulosa cells and theca interna cells of the growing and antral follicles. Moderate immunoexpression of PRA were observed in the large lutein cells and endothelial cells of the corpus haemorrhagicum and corpus luteum and in the stroma cells. At the end of pseudopregnancy (18 days) strong PRA reactions were detected in the small lutein cells of the regressed corpus luteum. Moderate to strong PRA immuno-expression were observed in the proliferated theca interna cells of the atretic antral follicles. The atretic large lutein cells of the regressed corpus luteum showed negative immunostaining for PRA. This study showed that the PRA positive small lutein cells of the regressed corpus luteum and the PRA positive proliferated theca interna cells of the atretic antral follicles were transformed into PRA positive interstitial gland cells. In conclusion, the present study had described the distribution of PRA in the ovary of pseudopregnant rabbit, which is not discussed before in the available literature. It also gives more information about follicular dynamic, formation and origin of interstitial glands, mechanism of ovulation, formation and regression of the corpus luteum.

Ovarian, uterine, and luteal vascular perfusions during follicular and luteal phases in the adult cyclic female rabbits with special orientation to their histological detection of hormone receptor

“…Since the endometrium is the main target for the steroid hormones [ 93 ], estrogen in conjunction with progesterone play a significant role in controlling the uterine function mediated by their binding with their specific receptors [ 94 ]. In this study, the area % of ER and PR expression in the luminal epithelium and endometrial glands are significantly higher during the FP than that during the LP.…”

Distribution of Estrogen and Progesterone Receptors in Ovary of Pregnant and Non-Pregnant Sunda Porcupines

“…The lutein cell of the corpus luteum in pregnant Sunda porcupine ovary also appeared to be immunoreactive against progesterone receptor antibodies. In mammals, progesterone has a role in the function of reproductive and follicular development [17,18]. Immunoreactivities of progesterone receptor in pregnant female Sunda porcupine ovary was detected in the corpus luteum and atretic follicles, while the immunoreactivities of progesterone receptor in nonpregnant Sunda porcupine ovary was detected in the theca internal cells of developed and atretic follicles.…”

Developmental events and cellular changes occurred during esophageal development of quail embryos

“…Detection of antigen localization was performed using a combination of the avidin–biotin complex technique 18 and the reagent of the Ultra Vision Detection System (Anti-Polyvalent, HRP/DAB manufactured by Thermo Fisher Scientific TP-015HD). The procedures were performed according to the manufacturer’s instructions 19 – 21 . Paraffin sections measuring 5 µm were dewaxed by xylene, hydrated with ascending grades of alcohol, and washed for 5 min twice with PBS (phosphate buffered saline) at pH 7.4 (Table 1 ).…”