The chemical composition and antibacterial activity of a methanolic extract of Satureja khuzistanica

Davoodi, Mansooreh; Ebrahimi, Samad Nejad; Rustaiyan, Abdolhossein; Esmaeili, Davoud

doi:10.1590/s2175-97902022e19233

Cited by 5 publications

(5 citation statements)

References 39 publications

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“…MS3 fragments at m/z 359 and 313 could result from caffeic acid moiety. According to previous works, this last fragment allowed the distinction of isomelitric acid A (peak 26)[69] from salvianolic acid J (peak 12)[56].Peaks 15 and 19 exhibited a UV profile characteristic of phenolic acids, in particular ferulic or caffeic acid derivatives, and shared the fragments at m/z 359 and 223 in their mass spectra. However, in peak 15, a molecular ion [M − H] − occurs at m/z 521, and the MS2 peak base at m/z 359 indicated the loss of a hexosyl moiety ([M − H − 162] − ).…”

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confidence: 68%

Effect of Thymbra capitata (L.) Cav. on Inflammation, Senescence and Cell Migration

et al. 2023

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Aromatic plants are reported to display pharmacological properties, including anti-aging. This work aims to disclose the anti-aging effect of the essential oil (EO) of Thymbra capitata (L.) Cav., an aromatic and medicinal plant widely used as a spice, as well as of the hydrodistillation residual water (HRW), a discarded by-product of EO hydrodistillation. The phytochemical characterization of EO and HRW was assessed by GC-MS and HPLC-PDA-ESI-MSn, respectively. The DPPH, ABTS, and FRAP assays were used to disclose the antioxidant properties. The anti-inflammatory potential was evaluated using lipopolysaccharide-stimulated macrophages by assessing NO production, iNOS, and pro-IL-1β protein levels. Cell migration was evaluated using the scratch wound assay, and the etoposide-induced senescence was used to assess the modulation of senescence. The EO is mainly characterized by carvacrol, while the HRW is predominantly characterized by rosmarinic acid. The HRW exerts a stronger antioxidant effect in the DPPH and FRAP assays, whereas the EO was the most active sample in the ABTS assay. Both extracts reduce NO, iNOS, and pro-IL-1β. The EO has no effect on cell migration and presents anti-senescence effects. In opposition, HRW reduces cell migration and induces cellular senescence. Overall, our study highlights interesting pharmacological properties for both extracts, EO being of interest as an anti-aging ingredient and HRW relevant in cancer therapy.

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confidence: 68%

Effect of Thymbra capitata (L.) Cav. on Inflammation, Senescence and Cell Migration

et al. 2023

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“…The tolerance to oxidative stress correlates with an increase in four specific secondary metabolites—quercitrin, linarin, citric acid, and 4-O-p-coumaroylquinic acid—each known to be effective against oxidative stress [ 19 , 20 , 21 , 22 , 23 ]. Tolerance to both bacteria and fungi correlates with five secondary metabolites—salicylic acid, kaempferol-7-O-glucoside, linarin, quercitrin, and ascorbigen, each of which shows strong antipathogen activities [ 23 , 24 , 25 , 26 , 27 , 28 , 29 ].…”

Section: Resultsmentioning

confidence: 99%

“…Among the secondary metabolites enriched in ICT1, we notice metabolites associated with resistance to oxidative stress and pathogen resistance. These include quercitrin, linarin, citric acid, and 4-O-p-coumaroylquinic acid, each known to be effective against oxidative stress [ 19 , 20 , 21 , 22 , 23 ], and salicylic acid, kaempferol-7-O-glucoside, linarin, quercitrin, ascorbigen, and three glucosinolates, including glucobrassicin, each of which show strong antipathogen activities [ 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 ]. Thus, the overexpression of S30 in ICT1 results in a higher relative abundance of metabolites associated with oxidative stress and pathogen tolerance, which provides a biochemical explanation for the observed corresponding phenotypes.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of S30 Ribosomal Protein Leads to Transcriptional and Metabolic Changes That Affect Plant Development and Responses to Stress

Finkelshtein,

Khamesa-Israelov,

Chamovitz

2024

Biomolecules

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ICT1 is an Arabidopsis thaliana line that overexpresses the gene encoding the S30 ribosomal subunit, leading to tolerance to exogenous indole-3-carbinol. Indole-3-carbinol (I3C) is a protective chemical formed as a breakdown of I3M in cruciferous vegetables. The overexpression of S30 in ICT1 results in transcriptional changes that prime the plant for the I3C, or biotic insult. Emerging evidence suggests that ribosomal proteins play important extra-ribosomal roles in various biochemical and developmental processes, such as transcription and stress resistance. In an attempt to elucidate the mechanism leading to I3C and stress resistance in ICT1, and using a multi-pronged approach employing transcriptomics, metabolomics, phenomics, and physiological studies, we show that overexpression of S30 leads to specific transcriptional alterations, which lead to both changes in metabolites connected to biotic and oxidative stress tolerance and, surprisingly, to photomorphogenesis.

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“…However, these compounds are extant in the analysed essential oil of the cultivated S. pilosa (24,); hence, the antimicrobial activity of S. pilosa is probably due to the presence of these strong antimicrobial volatiles, without excluding the possible contribution of polar phenolics. Quite recently, and similarly, the flavonoids diosmetin-7-rutinoside and linarin from S. khuzistanica were shown to exert synergistic interactions when combined with carvacrol [ 68 ]. Therefore, the actual synergism between the phenolics and the volatiles of S. pilosa might be an interesting idea to explore in the future.…”

Section: Discussionmentioning

confidence: 99%

HPLC- and NMR-Based Chemical Profiling, Wound-Healing Potential, Anti-Inflammatory and Antibacterial Activities of Satureja pilosa (Lamiaceae), a Neglected Medicinal–Aromatic Herb

Panagiotidou,

Burgers,

Tsadila

et al. 2023

Plants

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Satureja pilosa Velen. (Lamiaceae) is a perennial and melliferous aromatic–medicinal subshrub which is range-restricted in adjacent parts of Greece and Bulgaria and locally in Italy, known in Northern Greece as wild oregano (“agriorigani”) and traditionally collected from the wild for culinary purposes. Since the ethnopharmacological data and modern biological activities of Satureja spp. suggest promising applications in skin conditions, the present study aimed to investigate the hitherto unknown phenolic content of cultivated S. pilosa and its potential biological activities, focusing mainly on wound-healing and anti-inflammatory effects. An HPLC-PDA-MS-targeted phytochemical investigation, along with NMR, allowed for the isolation and characterization of the main constituents, resulting in 18 compounds. Representative extracts and purified compounds were tested for wound-healing activity on NIH/3T3 fibroblasts. The butanol extract exhibited a significantly higher cell migration rate (73.4%) compared to aqueous (50.6%) and methanolic (49.6%) ones, enhancing the cell migration more rapidly at both concentration levels, whilst rosmarinic acid was the most potent among the isolated compounds, with a migration rate of 64.0% at the concentration level of 10−5 mg/mL, followed by 3,4-dihydrophenyllactic acid (54.7%). Moreover, potential effects on endothelial activation processes were explored, including the leukocyte–endothelial cell interaction during inflammatory processes and the migratory capacity during angiogenic actions, since these processes are commonly associated with skin diseases. Finally, extracts and purified compounds demonstrated weak antibacterial potential against two important pathogens (Staphylococcus aureus and Pseudomonas aeruginosa), suggesting that further investigation is warrented.

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The chemical composition and antibacterial activity of a methanolic extract of Satureja khuzistanica

Cited by 5 publications

References 39 publications

Effect of Thymbra capitata (L.) Cav. on Inflammation, Senescence and Cell Migration

Effect of Thymbra capitata (L.) Cav. on Inflammation, Senescence and Cell Migration

Overexpression of S30 Ribosomal Protein Leads to Transcriptional and Metabolic Changes That Affect Plant Development and Responses to Stress

HPLC- and NMR-Based Chemical Profiling, Wound-Healing Potential, Anti-Inflammatory and Antibacterial Activities of Satureja pilosa (Lamiaceae), a Neglected Medicinal–Aromatic Herb

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