Development and validation of RP-HPLC method for simultaneous determination of lamivudine, stavudine, and zidovudine in perfusate samples: Application to the Single-Pass Intestinal Perfusion (SPIP) studies

Dezani, Thaisa Marinho; Dezani, André Bersani; Serra, Cristina Helena dos Reis

doi:10.1590/s2175-97902020000419073

Cited by 2 publications

(1 citation statement)

References 25 publications

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“…There are no interactions between NPXN and ESZL in terms of pharmacokinetics. While the NPXN/ESZL tablet has the same bioequivalence as an entericcoated naproxen tablet, its esomeprazole bioavailability is lower than that of an entericcoated version [2,3,13,34]. Therefore, the objective of this study is to develop a chromatographic method for the simultaneous detection of ESZL and NPXN in Kleprum® tablets, which are sold in the Jordanian pharmaceutical market.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Naproxen and Esomeprazole in Bulk and Pharmaceutical Tablets Dosage Form by RP-Chromatography

2023

JPTCP

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An original, trustworthy, simple, precise, rapid, sensitive, and accurate RP-HPLC method was validated for quantifying naproxen and esomeprazole in both conventional and synthetic mixed formulation tablets. The maximum absorbances of Naproxen and Esomeprazole in a 60:40 (vol/vol) mixture of deionized water and methanol were 244.8 and 272.6nm, respectively. To isolate naproxen from esomeprazole, a Shimadzu LC-20-A RP-HPLC is employed equipped with a C18 Phenomenex column (250mm 4.6mm 5 µm). Elution was done with a methanol-water eluent (40:60 V/V). Both naproxen and esomeprazole were eluted within (3.468 and 5.376 min, respectively) after a 10-minute separation at 270 nm using a UV-Vis detector and a flow rate of 1.0 mL/min. Elution-phase conditions were studied, including elution-phase composition, flow rate, pH, and wavelength. Naproxen and esomeprazole both had linearity ranges of 0.5 -25 µg/mL and 0.1 -30 µg/mL, respectively, with R2 values of 0.9995 and 0.9997, and means of recovery of 99.57 and 100.36, respectively. The method was used to samples of medications in different dosage forms in order to determine their gradient active. The average recovery % was used to determine whether the technique was accurate, and it was determined to be within a reasonable range.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Naproxen and Esomeprazole in Bulk and Pharmaceutical Tablets Dosage Form by RP-Chromatography

2023

JPTCP

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RP-HPLC Method for Dapagliflozin and Metformin HCL in Bulk and Combined Formulation

Bhavyasri¹,

Surekha²,

Begum³

et al. 2021

Arch. Pharm. Pract

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Development and validation of RP-HPLC method for simultaneous determination of lamivudine, stavudine, and zidovudine in perfusate samples: Application to the Single-Pass Intestinal Perfusion (SPIP) studies

Cited by 2 publications

References 25 publications

Evaluation of Naproxen and Esomeprazole in Bulk and Pharmaceutical Tablets Dosage Form by RP-Chromatography

Evaluation of Naproxen and Esomeprazole in Bulk and Pharmaceutical Tablets Dosage Form by RP-Chromatography

RP-HPLC Method for Dapagliflozin and Metformin HCL in Bulk and Combined Formulation

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