Development of amphotericin b Based organogels against mucocutaneous fungal infections

Abstract

Amphotericin B is a broad spectrum antifungal agent used to treat fungal infections. Organogel is a semisolid preparation in which the apolar phase gets immobilized within spaces of the threedimensional structure. The current study aimed at the formulation and comparative evaluation of sorbitan monostearate organogels and pluronic lecithin organogels (PLO). Different compositions of span 60 based organogels were prepared by varying the concentrations of the span 60 and PLO gels were prepared by varying the concentration of Pluronic F 127. The developed organogels were subjected to various characteristics such as pH, viscosity, spreadability, extrudability, and drug release studies. The optimized formulations were evaluated against Candida albicans and carried out ex vivo release study. The optimized formulation was selected from span 60 based organogels, and pluronic lecithin organogels were S1 and P1, respectively. The optimized formulation (S1) showed effective inhibition against Candida albicans. The skin irritation test was carried out on albino mice for optimized formulations and results showed that no irritation to the skin. Based on the results, organogels prepared by sorbitan monostearate showed better antifungal activity, and also all the formulations were found to be stable and safe throughout the study period.

Kombi̇ne Sali̇si̇li̇k Asi̇t Ve Povi̇don-İyot İçeren Nanoemüljelleri̇n Hazirlanmasi Ve Karakteri̇zasyonu: Ön Çalişma

“…When SA in the formulation passes from the oil phase in which it is soluble to the high viscosity aqueous phase in which it is insoluble, its release is slowed due to decreased solubility [35]. The polymer concentration that forms the hydrogel outside the droplets causes the release to be delayed due to the network-like structures formed in the gel [19]. In our study, after 8 hours, F1 released approximately 40%, while pure SA released approximately 45%.…”

“…Finally, a weight of 1 kg was placed on the upper plate and left for 5 minutes. The spreadability of the nanoemulgel was evaluated by measuring the increasing diameter at the end of time [19]. In order to determine the spreadability stability, the nanoemulgels were kept at room temperature for 1 month.…”

Curcumin Hybrid Lipid Polymeric Nanoparticles: Antioxidant Activity, Immune Cellular Response, and Cytotoxicity Evaluation

“…The PLU signals in the FT-IR spectra of CUR-NP and the Plain-NP correspond to 2896 cm −1 was associated to C-H stretch aliphatic, 1369 cm −1 was assigned to in-plane O-H bend, 1045 was related to C-O stretching of aliphatic ether group [ 25 ]. These signals provide information about the interaction between the polymer and the drug, if these compounds interact, then would be band shifts and broadening in the functional groups in the FT-IR spectra compared to the spectra of the free CUR and polymer [ 26 ]. The spectral analysis of Plain-NP and CUR-NP exhibited peaks which were a summation of the characteristic peaks obtained with the free CUR and pure components, indicating no interaction between the structural components of the system and CUR molecules.…”

Design of Hybrid Polymeric-Lipid Nanoparticles Using Curcumin as a Model: Preparation, Characterization, and In Vitro Evaluation of Demethoxycurcumin and Bisdemethoxycurcumin-Loaded Nanoparticles

“…The CUR-loaded PLHNs spectra indicated a combination of these signals from CUR as well as from Pluronic F-127 and Chol components. For instance, bands at 2897 cm −1 associated with C-H stretch aliphatic and at 1359 cm −1 corresponding to in-plane O-H bend pertain to Pluronic F-127 [ 48 ]. In addition, signals at 1449 cm −1 , 1043 cm −1 and 742 cm −1 correspond to CH 2 and CH 3 deformation vibrations, ring deformation, and C-H out-of-plane bending from Chol [ 49 ].…”

“…Comparing the FT-IR spectra of plain PLHNs, DMC, or BDM-loaded PLHNs with the ones obtained for pure DMC or BDM ( Figure 4 ), it becomes evident that they share peaks at wavenumbers of 3318 cm −1 associated with stretching vibration of hydrogen-bonded (-OH), as well as at 1659 and 1707 cm −1 stretching vibration of conjugated carbonyl (C=O) group, 1459 cm −1 and 1422 cm −1 CH- bending, and 1370 cm −1 and 1347 cm −1 to in-plane O-H bend, respectively, for DMC and BDM. Besides, characteristic bands at 2928 cm −1 associated to C-H stretch aliphatic corresponding to Pluronic F-127 [ 48 ] and signals at 1048 cm −1 and 754 cm −1 corresponding to ring deformation and C-H out-of-plane bending from Chol [ 49 ] are also present in the curcuminoids loaded PLHNs spectra. Therefore, these facts are to be expected due to the combination of signals of the components and the pure drug, which is further confirm that curcuminoids DMC and BDM were successfully loaded onto PLHNs core.…”