Design of Hybrid Polymeric-Lipid Nanoparticles Using Curcumin as a Model: Preparation, Characterization, and In Vitro Evaluation of Demethoxycurcumin and Bisdemethoxycurcumin-Loaded Nanoparticles

Abstract

Polymeric lipid hybrid nanoparticles (PLHNs) are the new generation of drug delivery systems that has emerged as a combination of a polymeric core and lipid shell. We designed and optimized a simple method for the preparation of Pluronic F-127-based PLHNs able to load separately demethoxycurcumin (DMC) and bisdemethoycurcumin (BDM). CUR was used as a model compound due to its greater availability from turmeric and its structure similarity with DMC and BDM. The developed method produced DMC and BDM-loaded PLHNs with a size average of 75.55 ± 0.51 and 15.13 ± 0.014 nm for DMC and BDM, respectively. An FT-IR analysis confirmed the encapsulation and TEM images showed their spherical shape. Both formulations achieved an encapsulation efficiency ≥ 92% and an exhibited significantly increased release from the PLHN compared with free compounds in water. The antioxidant activity was enhanced as well, in agreement with the improvement in water dissolution; obtaining IC50 values of 12.74 ± 0.09 and 16.03 ± 0.55 for DMC and BDM-loaded PLHNs, respectively, while free curcuminoids exhibited considerably lower antioxidant values in an aqueous solution. Hence, the optimized PHLN synthesis method using CUR as a model and then successfully applied to obtain DMC and BDM-loaded PLHNs can be extended to curcuminoids and molecules with a similar backbone structure to improve their bioactivities.

Bovine Serum Albumin-Based Nanoparticles: Preparation, Characterization, and Antioxidant Activity Enhancement of Three Main Curcuminoids from Curcuma longa

“…3 Different superscript letters in the same column or different superscript signs in the same row indicate differences are significant (p < 0.05) using one-way analysis of variance (ANOVA) with a Tukey post hoc. 4 Results for DMC and BDM were previously reported by our group [50].…”

Development of a Clioquinol Nanocarrier as a New, Promising Option for the Treatment of Dermatomycosis

“…Comparing the spectra of CQ and LBN-CQ ( Figure 3 e), the disappearance of characteristic bands of CQ at 1576 cm −1 and 1041 cm −1 and band shifts from 1605 cm −1 to 1639 cm −1 and from 1200 cm −1 to 1217 cm −1 were observed. The disappearance or shifts of characteristic bands from CQ can indicate interactions of the drug with the nanosystem, reiterating its presence in the nanocarrier [ 62 , 63 , 64 , 65 , 66 ]. These findings, along with the high encapsulation efficiency, could be an indication of drug encapsulation in the nanoformulation [ 62 , 63 , 64 , 65 , 66 ].…”

“…The disappearance or shifts of characteristic bands from CQ can indicate interactions of the drug with the nanosystem, reiterating its presence in the nanocarrier [ 62 , 63 , 64 , 65 , 66 ]. These findings, along with the high encapsulation efficiency, could be an indication of drug encapsulation in the nanoformulation [ 62 , 63 , 64 , 65 , 66 ].…”

The Antitumoral Effect In Ovo of a New Inclusion Complex from Dimethoxycurcumin with Magnesium and Beta-Cyclodextrin

“…[1] Abdelkader et at. (2022) [33] Ismail-Alhasawi (2022) [34] Fawzy et al On the other hand, an additional approach to solving the problem of poor solubility of curcuminoid-type molecules has been the discovery of new formulations, including liposomes [39,40], nanoparticles [41][42][43][44][45], or the preparation of inclusion complexes with cyclodextrins [46,47]. Beta-cyclodextrin (BCD) is the first choice [48] for the administration of drugs toward their sites of action because of its low toxicity [49], certain hydrophilicity [50], and adequate cavity.…”