The in vitro and in vivo investigation of biological activities and phenolic analysis of Helichrysum plicatum subsp. plicatum

Abstract

In Turkey, Helichrysum genus is represented by 26 taxa belonging to 20 species in Turkish flora of which 14 ones are endemic to Turkey. The aerial parts of Helichrysum plicatum subsp. plicatum are used kidney stones, kidney and stomach ailments. The extraction procedures and solvents are important step in processing of bioactive constituents from the plant materials. Therefore, the aim of this study was to evaluate in vitro antioxidant, antimicrobial, anti-urease, anticholinesterase and in vivo antiinflammatory activities of Helichrysum plicatum subsp. plicatum different extracts. In addition, the phenolic characterization of the Soxhlet and maceration methanol extracts which showed significant antioxidant, anti-urease, antimicrobial, anti-inflammatory and anticholinesterase activities were performed by HPLC-DAD and LC-MS/MS. In the present study, the Soxhlet methanol extract exhibited strong antioxidant, antimicrobial and anticholinesterase activities than other extracts. The maceration methanol extract showed the strongest anti-urease activity. The Soxhlet methanol and maceration methanol extracts showed in vivo anti-inflammatory activities very close to each other. As a result of this study, chlorogenic acid, dicaffeoylquinic acid, luteolin, luteolin-7-O-glucoside, naringenin-O-hexoside and isoquercitrin compounds were analysed in plant. Therefore, it is thought that methanol extracts can be used as a natural source in the future for food and pharmaceutical industries.

In vitro Antioxidant Activity and In vivo Anti-inflammatory Effect of Ricinus communis (L.) and Withania somnifera (L.) Hydroalcoholic Extracts in Rats

“…Antioxidants neutralize the free radical character of DPPH * by either transferring an electron or a hydrogen atom [34]. The reducing potential of compounds could indicate the possible antioxidant property [35]. Results of the present study indicated a gradual increase in scavenging activity with the increase of extract concentration.…”

IN VITRO STUDY ON ANTIMICROBIAL AND CYTOTOXIC ACTIVITY OF METHANOL EXTRACT of Helichrysum plicatum subsp. polyphyllum (Asteraceae)

“…It is known that extracts of H. plicatum have remarkable antioxidant, antimicrobial, and cytotoxic activities (Demir et al 2009;Tatlı et al 2009;Apaydın Yıldırım et al 2017;Taşkın et al 2020). In the literature, it is reported that the composition of H. plicatum is rich in flavonoids (Kulevanova et al 2000;Acet et al 2019;Güneş et al 2019).…”