Assessment of anti-diabetic activity of a novel hydrazine-thiazole derivative: in vitro and in vivo method

Abstract

Diabetes mellitus is a chronic disease resulting in oxidative stress that promotes tissue damage. The appearance of this disease is highly related to lifestyle and food of the population, being of great interest to search for a dietary supplement that can also act by reducing oxidative alterations. Based on the broad range of biological activity of thiazole derivatives, this work aimed to evaluate the in vitro antioxidant activity of a novel hydrazine-thiazole derivative and studies in vivo. In in vivo experiments, the liver extracts of healthy and diabetic Wistar rats were used, with analysis to determine the enzymatic activity of SOD, CAT, GPx, and GR, and determination of lipid peroxidation. Finally, in the blood of these animals, biochemical parameters were evaluated. Statistical evidence of changes caused in liver enzymes and liquid peroxidation was not detected; however, these parameters were also not changed between control groups with and without diabetes. On the other hand, concerning biochemical parameters, significant differences were detected in uric acid, alkaline phosphatase, ALT, and urea, indicating a possible antioxidant protective role of such substances in the liver and kidney of diabetic animals that could be acting by means other than that commonly reported in the literature.

Synthesis and in Silico Docking Studies of Ethyl 2‐(2‐Arylidene‐1‐alkylhydrazinyl)thiazole‐4‐carboxylates as Antiglycating Agents

“…Although, many previous reports represented thiazoles as potent antioxidant agents but in our study N ‐alkylated thiazole‐4‐carboxylates did not show antioxidant activity. A mechanistic representation of the reaction between DPPH and hydrazino‐thiazoles was reported in literature [35] given in Figure 4. The mechanistic representation highlighted, that the presence of −NH moiety is very much essential in a compound to show antioxidant behaviour.…”

Attenuation of Spatial Memory in 5xFAD Mice by Halting Cholinesterases, Oxidative Stress and Neuroinflammation Using a Cyclopentanone Derivative

“…Following up on this perspective the cyclopentanone derivative 2-(hydroxy-(3-nitrophenyl)methyl)cyclopentanone (3NCP, Figure 1 ) [ 12 ] was evaluated as a possible potential candidate against AD. The inhibition of AChE and BChE , also the antioxidant activities has been attributed to the presence of the nitrophenyl group [ 13 , 14 , 15 , 16 , 17 ]. The ketone moiety exhibited dose-dependent antioxidant effects, which have been considered as a possible explanation for the decline of chronic neurodegenerative disorders like AD [ 18 ].…”

Attenuation of spatial memory in 5xFAD mice by targeting cholinesterases, oxidative stress and inflammatory signaling using 2-(hydroxyl-(2-nitrophenyl)methyl)cyclopentanone

“…The 2NCP has a nitrophenyl group, and literature studies revealed that compounds containing nitrophenyl groups have the potential to inhibit AChE, BChE, monoamine oxidase-B (MAO-B), and relieve oxidative stress [30]. Whereas, the nitrobenzaldehyde derivatives exhibited antioxidant activity due to the nitro group attached to the benzene ring [31][32]. The 2NCPwas synthesized by the previously reported method [33][34].…”