Alpha amylase and Alpha glucosidase inhibitory effects of aqueous stem extract of Salacia oblonga and its GC-MS analysis

Chelladurai, Gladis Raja Malar; Chinnachamy, Chellaram

doi:10.1590/s2175-97902018000117151

Cited by 62 publications

(44 citation statements)

References 16 publications

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“…The methanolic and aqueous extract of S. oblonga showed effective inhibition against α-glucosidase ranging from 8.92% to 68.51% at a concentration of 10 to 100 mg/mL [17]. Apart from this, this study showed only 25.8% and 12.9% percentage inhibition of α-glucosidase in methanol and hexane extracts of W. somnifera.…”

Section: Enzyme Inhibition Assaymentioning

confidence: 48%

“…The α-glucosidase inhibition was determined by the modified methods by Chelladurai [17]. The 20 µL of α-glucosidase enzyme and 100 µL of phosphate buffered saline (pH 6.8, 0.05 M) and 60 µL of sample were pre-incubated with various concentrations of extracts at 37 ᵒ C. After the pre-incubation of 15 min, 20 µL substrate, p-nitrophenyl-a-D-glucopyranoside was added and reaction was carried out at 37 ᵒ C for 30 min.…”

Section: Enzyme Inhibition Assaymentioning

confidence: 99%

“…This is due to the availability of phytochemical constituents like tannins, terpenoids and flavonoids. Tannins and flavonoids have potential inhibitory effects on alpha amylase and alpha glucosidase [17].…”

Section: Enzyme Inhibition Assaymentioning

confidence: 99%

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FTIR, GC-MS Analysis and Bioactivity Studies of Withania somnifera L. of Nepalese Origin

Aryal¹,

Shrestha²,

Devkota³

et al. 2020

J. Nepal Chem. Soc.

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Present study evaluates the bio-constituents and bioactivity of extract from roots and stems of Withania somnifera (Aswagandha) of Nepalese origin. The phytochemical screening of methanol and n-hexane extracts revealed the presence of alkaloids, flavonoids, phenols, tannins, quinone, carbohydrates, resins, xanthoprotein etc. The FTIR analysis helped to confirm the functional groups of various medicinally important compounds. The DPPH percentage radical scavenging activity of methanolic and hexane extracts are found to be 3.587 ± 0.004, and 4.484 ± 0.003 µg/mL respectively. The antioxidant activity of the plant goes after the number of total phenolics and flavonoids present on it. GC-MS analysis revealed the presence of more than 14 different compounds in the extract. Moreover, the methanol and hexane extract of plant showed 25.8% and 12.9% α-glucosidase inhibitory activity respectively. The results of the study revealed the medicinal values and pharmacological significance of the plant.

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Section: Enzyme Inhibition Assaymentioning

confidence: 48%

Section: Enzyme Inhibition Assaymentioning

confidence: 99%

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FTIR, GC-MS Analysis and Bioactivity Studies of Withania somnifera L. of Nepalese Origin

Aryal¹,

Shrestha²,

Devkota³

et al. 2020

J. Nepal Chem. Soc.

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“…Based on the E value against different protein targets, the order of ligand affinity was found to be SGLT > AR > SIMI > PI3K > 11b-HSD1 > FBP1 > C-AG > PEPCK > GSK-3b > AA > PPAR-g. The enzyme alpha-amylase and alpha-glucosidase are responsible for the breakdown of carbohydrates and for hydrolyzing starch into glucose before absorption (Chelladurai and Chinnachamy, 2018). Reduction in postprandial hyperglycemic level is due to alpha-amylase inhibition, which delays the small intestine carbohydrate digestion and diminishes the postprandial blood glucose level (Kwon et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Computational and Pharmacological Evaluation of Carveol for Antidiabetic Potential

et al. 2020

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Background: Carveol is a natural drug product present in the essential oils of orange peel, dill, and caraway seeds. The seed oil of Carum Carvi has been reported to be antioxidant, anti-inflammatory, anti-hyperlipidemic, antidiabetic, and hepatoprotective. Methods: The antidiabetic potential of carveol was investigated by employing in-vitro, invivo, and in-silico approaches. Moreover, alpha-amylase inhibitory assay and an alloxaninduced diabetes model were used for in-vitro and in-vivo analysis, respectively. Results: Carveol showed its maximum energy values (≥-7 Kcal/mol) against sodiumglucose co-transporter, aldose reductase, and sucrose-isomaltase intestinal, whereas it exhibited intermediate energy values (≥-6 Kcal/mol) against C-alpha glucosidase, glycogen synthase kinases-3b, fructose-1,6-bisphosphatase, phosphoenolpyruvate carboxykinase, and other targets according to in-silico analysis. Similarly, carveol showed lower energy values (≥ 6.4 Kcal/mol) against phosphoenolpyruvate carboxykinase and glycogen synthase kinase-3b. The in-vitro assay demonstrated that carveol inhibits alpha-amylase activity concentration-dependently. Carveol attenuated the in-vivo alloxan-induced (1055.8 µMol/Kg) blood glucose level in a dose-and timedependent manner (days 1, 3, 6, 9, and 12), compared to the diabetic control group, and further, these results are comparable with the metformin positive control group. Carveol at 394.1 µMol/Kg improved oral glucose tolerance overload in rats compared to the hyperglycemic diabetic control group. Moreover, carveol also attenuated the glycosylated hemoglobin level along with mediating anti-hyperlipidemic and hepatoprotective effects in alloxan-induced diabetic animals. Conclusions: This study reveals that carveol exhibited binding affinity against different targets involved in diabetes and has antidiabetic, anti-hyperlipidemic, and hepatoprotective actions.

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“…Our GCMS results are in accordance with that of previously reported. [35][36][37][38] These compounds identified in our study belong to tannins, saponins, glycosides, fatty acids, esters of fatty acids, etc. Various therapeutic properties of the identified compounds have been reported and are being used in medical formulation and various other applications.…”

Section: Discussionmentioning

confidence: 99%

Effect of Solvents on Phytochemical Composition and Antioxidant Activity of Cardiospermum halicacabum (L.) Extracts

Dowlath¹,

Karuppannan²,

Gi³

et al. 2020

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Cardiospermum L. is a genus belonging to Sapindaceae family. It comprises about 17 species of herbs, vines distributed in the tropical regions of the earth. 15 Cardiospermum halicacabum (C. halicacabum) is an herbaceous climber from Sapindaceae family. It is widely distributed in the tropics and subtropics of India, Bangladesh, Pakistan, Malacca, Africa and America. It is one such plant which is traditionally used in Ayurvedic and folk medicines. 16 This plant is known to possess various biological and pharmacological properties. Various studies have shown the antimicrobial, antioxidant, analgesic, vasodepressant, anti-inflammatory properties of this plant. It is commonly used for treating arthritis, rheumatism, limbs stiffness, diabetes, lumbago, earache, fever. 17,18 It is the bioactive compounds present in the extracts of leaves, seeds, roots of this plant which contributes for the activities. 19,20 Various pharmacological properties of the phytoconstituents of this plant has been studied. Several chemical constituents such as i.e., apigenin, apigenin-7-Oglucuronide, chrysoeriol-7-O-glucuronide, luteolin-7-O-glucuronide rutin, and β-arachidic acid 21 have ABSTRACT Background: Cardiospermum halicacabum (C. halicacabum) is a common medicinal herb found in India and other Asian countries. It has various medicinal properties such as antimicrobial, pain relief, antibiotics, anti-inflammatory, antioxidants, anticancer etc. It is commonly used for treating diabetes, arthritis, limbs stiffness, rheumatism, lumbago, earache, fever. Type of solvent and polarity intensively affects the antioxidant activity of the extracts due to the solubility of the phytocompounds such as polyphenols and flavonoids in various solvents. Materials and Methods: In this study, different solvents like, ethanol, methanol, chloroform and petroleum ether were used for the extraction of C. halicacabum. Cold maceration method was followed for extraction. The crude extracts were screened preliminary and then confirmed using Fourier transform-infrared spectroscopy analysis. Gas chromatography-mass spectrometry was used to determine the chemical composition of each extract. The DPPH (2,2-diphenyl-1-picrylhydrazyl) method was used for the evaluation of the antioxidant activity of different crude extracts of C. halicacabum. Results: The results showed that there is significant influence of solvent type in preserving various phytocompounds of the C. halicacabum leaves extract. The evaluation of the antioxidant capacity of different crude extracts was in the order of ethanol > methanol > petroleum ether > chloroform extract.

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Alpha amylase and Alpha glucosidase inhibitory effects of aqueous stem extract of Salacia oblonga and its GC-MS analysis

Cited by 62 publications

References 16 publications

FTIR, GC-MS Analysis and Bioactivity Studies of Withania somnifera L. of Nepalese Origin

FTIR, GC-MS Analysis and Bioactivity Studies of Withania somnifera L. of Nepalese Origin

Computational and Pharmacological Evaluation of Carveol for Antidiabetic Potential

Effect of Solvents on Phytochemical Composition and Antioxidant Activity of Cardiospermum halicacabum (L.) Extracts

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