Chemical composition, antioxidant and biological activity of Ocotea bicolor Vattimo-Gil (LAURACEAE) essential oil

Abstract

The essential oil composition of the Ocotea bicolor, native plant of Brazil, was studied for the first time. The essential oil of the leaf was obtained by hydrodistillation and analyzed by GC/MS. The analytical procedure revealed a predominance of sesquiterpenes, δ-cadinene (7.39%), β-sesquiphellandrene (6.67%), β-elemene (5.41%) and α-cadinol (5,23%). The essential oil was submitted to brine shrimp toxicity evaluation, antioxidant and antibacterial tests. The antioxidant activity by the formation of phosphomolybdenum complex method presented positive results. The minimum inhibitory concentration (MIC) values were higher than 1000 μg/mL for the microorganisms tested. Toxicity activity revealed LC50 results of 40.10 (μg/mL), being toxic to the organisms in this study.

Composition, antioxidant properties, and biological activities of the essential oil extracted from Ocotea diospyrifolia (Meisn.) Mez.

“…A similar response profile was observed by Damasceno et al (2018) for the essential oil of Ocotea bicolor leaves. This species presented low activity in the DPPH assay (IC 50 > 500 µg/mL), but an antioxidant capacity comparable to the ascorbic acid (102.5%) in the phosphomolybdenum method (Damasceno et al, 2018).…”

“…A similar response profile was observed by Damasceno et al (2018) for the essential oil of Ocotea bicolor leaves. This species presented low activity in the DPPH assay (IC 50 > 500 µg/mL), but an antioxidant capacity comparable to the ascorbic acid (102.5%) in the phosphomolybdenum method (Damasceno et al, 2018). In contrast, the essential oil from Ocotea quixos and Ocotea bofo calyces inhibited 52% and 64.4% of the DPPH oxidation, respectively (Bruni et al, 2003;Guerrini et al, 2006).…”

“…The preliminary in vitro toxicity test showed a low activity against Artemia salina (Table II), according to the stratification system by Amarante et al (2011). The toxicity against brine shrimp has already been described for other Ocotea species: O. praetermissa-IC 50 : 31.6 µg/mL, O. endresiana-IC 50 : 6.9 µg/mL (Agius et al, 2007), O. notate-IC 50 : 2.3 7µg/mL (Garrett et al, 2010), O. bicolor-IC 50 : 40.10 µg/mL (Damasceno et al, 2018).…”

“…The essential oils extracted from the Ocotea genus have demonstrated potential in the biological and pharmacological activities field. Studies have shown positive results regarding antioxidant, antimicrobial (Bruni et al, 2004;Cansian et al, 2010;Salleh, Ahmad, 2017), cytotoxic, anti-inflammatory, cardiovascular, molluscicidal, and anti-malarial activities (Salleh, Ahmad, 2017) as well as toxicity against Artemia salina (Damasceno et al, 2018).…”

GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps

“…In addition, they found that both essential oils were more effective against Gram-positive bacteria, corroborating the findings of the present research. On the other hand, a study by Damascemo et al [36] found clinically ineffective MIC values for the essential oil of O. bicolor against S. aureus, P. aeruginosa, and E. coli. Furthermore, Cansian et al [37] found that an essential oil obtained from O. odorifera was more efficient against Gram-negative bacteria.…”