Effects of homologous and heterologous rich platelets plasma, compared to poor platelets plasma, on cutaneous healing of rabbits

Meira, Raquel de Oliveira; Braga, Daniel Nogueira Mendes; Pinheiro, Leni Safira Gonçalves; Amorim, Izabela Ferreira Gontijo de; Vasconcellos, Leonardo de Souza; Alberti, Luiz Ronaldo

doi:10.1590/s0102-865020200100000006

Cited by 4 publications

(5 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is thus a reason to follow up on the wound healing concepts to test heterologous preparations of PRF and translate this into a bone regeneration model such as the calvaria defect. In contrast to the two wound healing studies [35,43], we show that rat (but not human) PRF supports the osteoconductive properties of a collagen membrane when placed in a rat calvaria defect. The underlying reason remains unclear, but we know that collagen membranes functionalized with the conditioned medium of human PRF-thus, not containing the cells and the fibrin-rich matrix of the PRF membranes-result in around 50% defect coverage after four weeks [30].…”

Section: Discussioncontrasting

confidence: 99%

“…In wound healing, at least two studies have compared human platelet-rich plasma (PRP) and PRF with homologous preparations. For instance, human PRP showed more tissue retraction at the wound healing process's beginning than rabbit PRP [35]. In dog skin punch defects, PRP gels of human and dog origin were equivalent in their ability to accelerate wound healing after 17 days [43].…”

Section: Discussionmentioning

confidence: 98%

“…Coagulation allows the formation of a conglomerate of fibrin-rich matrix where all the ingredients are entrapped, allowing for good handling of the graft; presumably, the PRF components support the process of graft consolidation, which is essentially bone regeneration. Some preclinical studies have investigated wound healing using human platelet-rich fibrin (PRF) to assess its effects [34,35]. The rationale for using human donors stems from limitations such as the lower volume of PRF and faster coagulation compared to animal models [36].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Human versus Rat PRF on Collagen Membranes: A Pilot Study of Mineralization in Rat Calvaria Defect Model

Apaza Alccayhuaman,

Heimel,

Tangl

et al. 2024

Bioengineering

View full text Add to dashboard Cite

Platelet-rich fibrin, the coagulated plasma fraction of blood, is commonly used to support natural healing in clinical applications. The rat calvaria defect is a standardized model to study bone regeneration. It remains, however, unclear if the rat calvaria defect is appropriate to investigate the impact of human PRF (Platelet-Rich Fibrin) on bone regeneration. To this end, we soaked Bio-Gide® collagen membranes in human or rat liquid concentrated PRF before placing them onto 5 mm calvarial defects in Sprague Dawley rats. Three weeks later, histology and micro-computed tomography (μCT) were performed. We observed that the collagen membranes soaked with rat PRF show the characteristic features of new bone and areas of mineralized collagen matrix, indicated by a median mineralized volume of 1.5 mm3 (range: 0.9; 5.3 mm3). Histology revealed new bone growing underneath the membrane and hybrid bone where collagen fibers are embedded in the new bone. Moreover, areas of passive mineralization were observed. The collagen membranes soaked with human PRF, however, were devoid of histological features of new bone formation in the center of the defect; only occasionally, new bone formed at the defect margins. Human PRF (h-PRF) caused a median bone volume of 0.9 mm3 (range: 0.3–3.3 mm3), which was significantly lower than what was observed with rat PRF (r-PRF), with a BV median of 1.2 mm3 (range: 0.3–5.9 mm3). Our findings indicate that the rat calvaria defect model is suitable for assessing the effects of rat PRF on bone formation, but caution is warranted when extrapolating conclusions regarding the efficacy of human PRF.

show abstract

Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Human versus Rat PRF on Collagen Membranes: A Pilot Study of Mineralization in Rat Calvaria Defect Model

Apaza Alccayhuaman,

Heimel,

Tangl

et al. 2024

Bioengineering

View full text Add to dashboard Cite

show abstract

“…Moreover, there are situations where animals, as humans, have physiologic conditions compromised and may not be possible to collect their own blood in enough amounts for autologous PRP. In those situations, heterologous PRP (obtained from other species) may be useful, as in rabbits, where canine PRP contributed to wound healing similar to homologous PRP, with no adverse effects [ 18 , 19 ]. Thus, it would also be plausible to use PRP of human origin.…”

Section: Introductionmentioning

confidence: 99%

Human Platelet-Rich Plasma Regulates Canine Mesenchymal Stem Cell Migration through Aquaporins

Parascandolo

Tolla

Liguoro

et al. 2023

Stem Cells International

View full text Add to dashboard Cite

Platelet products are commonly used in regenerative medicine due to their effects on the acceleration and promotion of wound healing, reduction of bleeding, synthesis of new connective tissue, and revascularization. Furthermore, a novel approach for the treatment of damaged tissues, following trauma or other pathological damages, is represented by the use of mesenchymal stem cells (MSCs). In dogs, both platelet-rich plasma (PRP) and MSCs have been suggested to be promising options for subacute skin wounds. However, the collection of canine PRP is not always feasible. In this study, we investigated the effect of human PRP (hPRP) on canine MSCs (cMSCs). We isolated cMSCs and observed that hPRP did not modify the expression levels of the primary class of major histocompatibility complex genes. However, hPRP was able to increase cMSC viability and migration by at least 1.5-fold. hPRP treatment enhanced both Aquaporin (AQP) 1 and AQP5 protein levels, and their inhibition by tetraethylammonium chloride led to a reduction of PRP-induced migration of cMSCs. In conclusion, we have provided evidence that hPRP supports cMSC survival and may promote cell migration, at least through AQP activation. Thus, hPRP may be useful in canine tissue regeneration and repair, placing as a promising tool for veterinary therapeutic approaches.

show abstract

“…The PRP has been extensively studied as a new biological matrix able to promote wound healing across a variety of tissues, including skin, muscle, bone, cartilage, and even tendon lesions, with promising results [7]. PRP is a plasma component that contains 3-7 times as many platelets as whole blood [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Autologous and Homologous Platelets-Rich Plasma Gel on Cutaneous Wound Healing In Rescued Donkeys: A Comparative Study

Sajjad

Akbar²,

Hayat³

et al. 2022

Egyptian Journal of Veterinary Sciences

View full text Add to dashboard Cite

B ACKGROUND: Cutaneous wound healing in equine is frequently delayed and complicated. Therefore, a new therapy is needed to achieve rapid and satisfactory healing. Aims: This study aimed to investigate whether the use of autologous PRP gel in cutaneous wounds enhances wound repair and alters oxidative stress cascades than homologous PRP-gel in the rescued donkeys. Methods: 10 rescued donkeys were selected and allocated into two groups A and B. Group-A donkeys (n=5) were treated with APRP-gel, while group-B donkeys (n=5) were treated with HPRP-gel. A full-thickness (4 mm 2 ) skin wounds were selected in each donkey. Wound re-epithelialization was assessed by H&E staining and collagen reestablishment was assessed by Masson's trichrome staining. The CAT activity and MDA concentration were assessed in blood samples on days 7, 21, and 36. Results: We observed a significant increase in re-epithelialization, fibroblasts and angiogenesis from days 21 (P < 0.05) to 36 (P < 0.01) in APRP-wounds than HPRP-wounds. Collagen organization in APRPwounds was good (P<0.05) from days 21 to 36 than HPRP-wounds. Malondialdehyde (MDA) concentrations in APRP-wounds were significantly decreased from day 21 to day 36 compared with HPRP-wounds (P<0.01). There was no difference in catalase (CAT) activity between APRP and HPRP wounds (P>0.05). Conclusion: APRP-wounds improved cutaneous wound healing in rescued donkeys by reducing oxidative stress, speeding wound epithelialization, and developing more structured tissue with interlocking collagen bundles than HPRP wounds. However, further ultrasonography study is required to investigate the effect of PRP gel on cutaneous wound healing in rescued donkeys.

show abstract

Effects of homologous and heterologous rich platelets plasma, compared to poor platelets plasma, on cutaneous healing of rabbits

Cited by 4 publications

References 18 publications

Human versus Rat PRF on Collagen Membranes: A Pilot Study of Mineralization in Rat Calvaria Defect Model

Human versus Rat PRF on Collagen Membranes: A Pilot Study of Mineralization in Rat Calvaria Defect Model

Human Platelet-Rich Plasma Regulates Canine Mesenchymal Stem Cell Migration through Aquaporins

The Effect of Autologous and Homologous Platelets-Rich Plasma Gel on Cutaneous Wound Healing In Rescued Donkeys: A Comparative Study

Contact Info

Product

Resources

About