Effect of short-term ischemia on microcirculation and wound healing of adipocutaneous flaps in the rat

Molnár, Ábel; Magyar, Zsuzsanna; Nachmias, David Belkin; Mann, Din; Szabó, Balázs; Tóth, L; Németh, Norbert

doi:10.1590/s0102-865020190120000003

Cited by 2 publications

(2 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Blood flow maintenance is the main advantage of skin flaps compared with grafts, providing nutrients to cell proliferation/differentiation, cytokines and growth factors that control the healing. However, any microvasculature disturbance may results in flap failure and impairs the healing [ 58 ]. The anesthetic-dependence as an invasive therapy is other disadvantage of flaps compared with non-surgical treatments.…”

Section: Wound Treatmentsmentioning

confidence: 99%

See 1 more Smart Citation

Cutaneous Wound Healing: An Update from Physiopathology to Current Therapies

Gushiken

Beserra

Bastos

et al. 2021

Life

101

View full text Add to dashboard Cite

The skin is the biggest organ of human body which acts as a protective barrier against deleterious agents. When this barrier is damaged, the organism promotes the healing process with several molecular and cellular mechanisms, in order to restore the physiological structure of the skin. The physiological control of wound healing depends on the correct balance among its different mechanisms. Any disruption in the balance of these mechanisms can lead to problems and delay in wound healing. The impairment of wound healing is linked to underlying factors as well as aging, nutrition, hypoxia, stress, infections, drugs, genetics, and chronic diseases. Over the years, numerous studies have been conducted to discover the correct approach and best therapies for wound healing, including surgical procedures and non-surgical treatments such as topical formulations, dressings, or skin substitutes. Thus, this general approach is necessary to facilitate the direction of further studies. This work provides updated concepts of physiological mechanisms, the factors that can interfere, and updated treatments used in skin wound healing.

show abstract

Section: Wound Treatmentsmentioning

confidence: 99%

“…The anesthetic-dependence as an invasive therapy is other disadvantage of flaps compared with non-surgical treatments. Then, a high knowledge of skin flaps physiology and biomechanics is essential to the success of this method [ 58 , 59 ].…”

Section: Wound Treatmentsmentioning

confidence: 99%

Cutaneous Wound Healing: An Update from Physiopathology to Current Therapies

Gushiken

Beserra

Bastos

et al. 2021

Life

101

View full text Add to dashboard Cite

show abstract

Systematic review of pathologic markers in skin ischemia with and without reperfusion injury in microsurgical reconstruction: Biomarker alterations precede histological structure changes

Khalaf,

Duarte Bateman,

Reyes

et al. 2024

Microsurgery

View full text Add to dashboard Cite

BackgroundIschemia and ischemia–reperfusion injury contribute to partial or complete flap necrosis. Traditionally, skin histology has been used to evaluate morphological and structural changes, however histology does not detect early changes. We hypothesize that morphological and structural skin changes in response to ischemia and IRI occur late, and modification of gene and protein expression are the earliest changes in ischemia and IRI.MethodsA systematic review was performed in accordance with PRISMA guidelines. Studies reporting skin histology or gene/protein expression changes following ischemia with or without reperfusion injury published between 2002 and 2022 were included. The primary outcomes were descriptive and semi‐quantitative histological structural changes, leukocyte infiltration, edema, vessel density; secondary outcomes were quantitative gene and protein expression intensity (PCR and western blot). Model type, experimental intervention, ischemia method and duration, reperfusion duration, biopsy location and time point were collected.ResultsOne hundred and one articles were included. Hematoxylin and eosin (H&E) showed inflammatory infiltration in early responses (12–24 h), with structural modifications (3–14 days) and neovascularization (5–14 days) as delayed responses. Immunohistochemistry (IHC) identified angiogenesis (CD31, CD34), apoptosis (TUNEL, caspase‐3, Bax/Bcl‐2), and protein localization (NF‐κB). Gene (PCR) and protein expression (western blot) detected inflammation and apoptosis; endoplasmic reticulum stress/oxidative stress and hypoxia; and neovascularization. The most common markers were TNF‐α, IL‐6 and IL‐1β (inflammation), caspase‐3 (apoptosis), VEGF (neovascularization), and HIF‐1α (hypoxia).ConclusionThere is no consensus or standard for reporting skin injury during ischemia and IRI. H&E histology is most frequently performed but is primarily descriptive and lacks sensitivity for early skin injury. Immunohistochemistry and gene/protein expression reveal immediate and quantitative cellular responses to skin ischemia and IRI. Future research is needed towards a universally‐accepted skin injury scoring system.

show abstract

Effect of short-term ischemia on microcirculation and wound healing of adipocutaneous flaps in the rat

Abstract: Technical procedures, analysis and interpretation of data, manuscript preparation.

Cited by 2 publications

References 38 publications

Cutaneous Wound Healing: An Update from Physiopathology to Current Therapies

Cutaneous Wound Healing: An Update from Physiopathology to Current Therapies

Systematic review of pathologic markers in skin ischemia with and without reperfusion injury in microsurgical reconstruction: Biomarker alterations precede histological structure changes

Contact Info

Product

Resources

About