Effects of cyclosporine on ischemia-reperfusion injuries in rat kidneys. An experimental model

Abstract

PurposeTo assess Cyclosporine A (CsA) therapy at an intraperitoneal dose of 15 mg.kg -1 in a rodent model of non-septic renal ischemia.MethodsTwenty male Wistar rats were randomized to receive CsA therapy or none therapy before undergoing 30 minutes of renal ischemia followed by reperfusion. Additionally, 10 rats were randomized to undergo the same surgical procedure of the aforementioned animals with neither ischemia nor CsA therapy. Twelve hours after kidney ischemia, the left kidneys were evaluated for histological injury according to Park’s criteria. Serum creatinine (Cr), urea nitrogen (Ur) and sodium levels were obtained at different times of the experimental protocol.ResultsRodents in the CsA group showed negative results (p<0.05) in serum variables (Cr: 0.41±0.05mg/dL vs . 4.17±1.25mg/dL; Ur: 40.90±3.98mg/dL vs . 187.70±22.93mg/dL) even the non CsA or control group (Cr: 0.35±0.07mg/dL vs . 3.80±1.20mg/dL; Ur: 40.10±4.70mg/dL vs . 184.50±49.80mg/dL). The negative results were also verified in histological evaluation, CsA group had 50% in the very severe grade of lesion, 10% in the severe and 40% in the moderate to severe whereas the control group had 90% in the very severe grade.ConclusionCsA was incapable of preventing the deleterious effects of ischemia-reperfusion injury in rat kidneys.

The Efficiency of Oxerutin on Apoptosis and Kidney Function in Rats with Renal Ischemia Reperfusion Injury

“…[5,17] Serum levels of biomolecules such as BUN, urea, creatinine and Cys C are elevated in ARF. [14,[18][19][20] In this study, the findings that the mean BUN and urea levels in the RIRI group were significantly higher than those in the sham control group indicated that the experimental model was established correctly. The similarity of the mean BUN and urea levels in the RIRI + oxerutin and sham control groups supported to the protective effect of oxerutin in RIRI.…”

“…[12] Many studies have shown that the duration of ischemia for the development of RIRI is 30 min. [13][14][15] However, reperfusion times ranging from 1 h to 24 h were evaluated in the studies. [13][14][15][16] Since we considered that the improvement of early-stage RIRI will be a positive recovery sign for the late-stage renal functions, we conducted our RIRI model in such a way that 30 min of ischemia followed by 1 h of reperfusion.…”

“…[13][14][15] However, reperfusion times ranging from 1 h to 24 h were evaluated in the studies. [13][14][15][16] Since we considered that the improvement of early-stage RIRI will be a positive recovery sign for the late-stage renal functions, we conducted our RIRI model in such a way that 30 min of ischemia followed by 1 h of reperfusion. In the histopathological examinations and apoptotic index evaluations, the kidney tissues in the sham control group had a normal histopathological appearance and low apoptotic index, but those in the RIRI and RIRI + oxerutin groups exhibited deteriorated histopathological structure and apoptotic index at varying levels.…”

Nephroprotective Efficacy of Sugammadex in Ischemia-Reperfusion Injury: An Experimental Study in a Rat Model

“…As a result of this study, they stated that dexmedetomidine may have a protective effect on renal IR damage, but there is no such evidence for remifentanil [ 17 ]. In a recently published study, it has been reported that cyclosporine A given 15 mg/kg intraperitoneally does not prevent the harmful effects of IR damage in rat kidneys [ 18 ].…”

Effects of cyclosporin A pre-treatment combined with etomidate post-treatment on lung injury induced by limb ischemia-reperfusion in rats

“…It is an active metabolite of fungi in soil and has strong immunosuppressive effects. [20][21][22] CsA can reversibly and selectively alter the function of T-lymphocytes, prevent the transcription of lymphokine genes, interfere with signaling, 19 inhibit release of IL-2, interferon and other immune factors, alter humoral and cellular immunity, inhibit the killing activity of natural killer cells, and inhibit the differentiation and proliferation of lymphocytes. 23,24 ETO is used for general anesthesia and is a safe imidazole derivative.…”