Undernutrition impairs the quality of growth plate and trabecular and cortical bones in growing rats

Guedes, Patrícia Madalena San Gregório; Zamarioli, Ariane; Botega, Iara Inácio; Silva, Léa Assed Bezerra da; Issa, João Paulo Mardegan; Butezloff, Mariana Maloste; Sousa, Yara Terezinha Corrêa Silva; Ximenez, João Paulo Bianchi; Volpon, José Batista

doi:10.1590/s0102-865020190030000001

Cited by 10 publications

(7 citation statements)

References 31 publications

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“…Some studies have shown that decreased bone formation is the predominant mechanism (47), while others point to increased bone resorption as the main cause of bone loss (49). Bilateral oophorectomy, spinal cord lesion, immobilization and dietary manipulation are the most common methods used to induce experimental osteoporosis (50)(51)(52)(53)(54)(55)(56). While we did not use the most common models of experimental osteoporosis described in the literature, our trabecular bone loss was equivalent to these classical models, as depicted by our microCT results, that showed severe bone loss in the OG.…”

Section: Discussionmentioning

confidence: 99%

Ex vivo vibro-acoustography characterization of osteoporosis in an experimental mice model

Agnollitto¹,

Braz²,

Spirlandeli³

et al. 2021

Quant Imaging Med Surg

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Background: Osteoporosis is a highly prevalent multifactorial osteometabolic disease, classically diagnosed, in vivo, by dual energy X-ray absorptiometry (DXA). This study evaluated osteoporosis, ex vivo, using vibroacoustography (VA), an elastographic technique based on ultrasound radiation force.Methods: Three groups of mice femurs were used: (I) control group (CG), (II) osteoporosis group (OG) and (III) treated osteoporosis group (TOG), in which the animals received pamidronate, an antiresorptive drug. Evaluation was performed in an acoustic tank, using two high frequency focused beams produced by a confocal ultrasonic transducer. A hydrophone registered the low frequency acoustic response (AR) of bone samples. We used micro-computed tomography (microCT) as the reference standard and evaluated the correlation between VA and microCT parameters. Results:The spectral analyses of the ARs with estimated area under the curve (AUC) values (mean; st. dev.) were, respectively, 1.29e -07 and 9.32e -08 for the CG, 3.25e -08 and 2.16e -08 for the OG, and 1.50e -07 and 8.37e -08 for the TOG. VA differentiated the experimental groups (P<0.01) and the results were reproducible [interclass correlation coefficient (ICC): 0.43 (95% CI: 0.15-0.71)]. There was also a statistically significant association between VA and microCT connectivity (Conn.) (r=0.80; P<0.01) and connectivity density (Conn. D) (r=0.76; P<0.01).Conclusions: These results encourage further studies aimed at evaluating the potential use of VA for the diagnosis of osteoporosis as a relatively low-cost and radiation-free alternative to DXA.

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Section: Discussionmentioning

confidence: 99%

Ex vivo vibro-acoustography characterization of osteoporosis in an experimental mice model

Agnollitto¹,

Braz²,

Spirlandeli³

et al. 2021

Quant Imaging Med Surg

View full text Add to dashboard Cite

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“…Частковий чи повний дефіцит однієї зі складових раціону може спричинити виникнення аномального розвитку кісткової тканини [1]. При цьому виникають порушення щільності, міцності, зміни показників неорганічного та органічного матриксу [2][3][4]. Вплив дефіциту раціону на кісткову тканину добре вивчений у дорослих гризунів.…”

Section: вступunclassified

“…Березовський раціону на 50% призводило до зменшення об'єму ростової пластинки кісткової тканини молодих щурів, її загальної площі і висоти, зниження маси та щільності трабекулярної і кортикальної кістки. Проте обмеження раціо ну харчування не впливало на експресію гена колагену першого типу (Col1a1), фактора транскрипції, пов'язаного з Runt 2 (Runx2) [3]. Втрата маси при щоденному обмеженні калорійності раціону не завжди призводить до зниження кісткової маси і до негативних змін маркерів метаболізму кісткової тканини [6].…”

Section: вступunclassified

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Influence of Two Options of Dosed Alimentary Deprivation on the Inorganic and Organic Bone Tissue Matrix Ya Young Rats

Litovka¹,

Vesselsky²,

Magomedov³

et al. 2020

Fiziol Zh

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“…Gráfico 4 -Gráficos de dispersão demonstrando demais correlações entre a vibroacustografia e o microCT em (a) BV/TV (r=0,19; p<0,47) e (b) espessura trabecular de conectividade (r=-0,34; p<0,20). GUEDES et al, 2019;MA, 1999;YAO, 2001;KOMORI, 2015;LELOVAS et al, 2008;MANDARANO-FILHO;BEZUTI;VOLPON et al, 2015;ZAMARIOLI et al, 2013). Embora não tenhamos utilizado os modelos mais comuns de osteoporose experimental da literatura, a perda óssea trabecular obtida foi equivalente a esses outros modelos.…”

Section: Correlações Entre Vibro-acustografia E Microctunclassified

Caracterização da resposta acústica por vibro-acustografia em fêmures de camundongos em um modelo experimental ex-vivo

Agnollitto¹

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Introduction: This study aimed to evaluate the potential of vibro-acustography (VA) technique to detect bone alterations associated with osteoporosis, using an animal model. Methods: The experimental protocol included three groups of mice: a) control group, b) group with osteoporosis and c) group with osteoporosis and treated with pamidronate, an antiresorptive drug. The evaluation of bone by the VA technique was conducted in an acoustic tank. A confocal ultrasonic transducer generated two high frequency (MHz) focused beams, with a difference frequency of 65 KHz between them. These two beams interact with each other and with the bone sample, producing a low frequency acoustic response registered by a hydrophone. The VA spectral curves were processed with the fast Fourier transform, in order to obtain numerical values that are weighted in the mechanical properties of the samples. VA acquisition was repeated three times. We used microCT as the reference technique and estimated the correlation of VA and microCT parameters. Statistical analysis included Interclass Correlation Coefficient (ICC), ANOVA multiple comparisons and Spearman's rank correlation coefficient. Results: VA spectral analysis and area under the curve values (mean; st. desv.) were, respectively, 1.29e-07 and 9.32e-08 for the control group, 3.25e-08 and 2.16e-08 for the group with osteoporosis; and 1.50e-07 and 8.37e-08 for the group with osteoporosis treated with pamidronate. VA differentiate the experimental groups (p<0.01) and the results were reproducible (ICC = 0.43 [95%CI = 0.15-0.71]). There was a statistically significant relationship between VA and MicroCT in connectivity (p<0.01; r=0.80) and connectivity density (p<0.01; r=0.76) and a trend between VA and trabecular number (Tb.N) and trabecular s Tb.Sp (p=0.06). Conclusion: VA differentiated the bones from the control group and the osteoporotic group. In addition, we found a strong positive correlation between VA and bone connectivity measured by microCT.

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Undernutrition impairs the quality of growth plate and trabecular and cortical bones in growing rats

Cited by 10 publications

References 31 publications

Ex vivo vibro-acoustography characterization of osteoporosis in an experimental mice model

Ex vivo vibro-acoustography characterization of osteoporosis in an experimental mice model

Influence of Two Options of Dosed Alimentary Deprivation on the Inorganic and Organic Bone Tissue Matrix Ya Young Rats

Caracterização da resposta acústica por vibro-acustografia em fêmures de camundongos em um modelo experimental ex-vivo

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