Healing effect of andiroba-based emulsion in cutaneous wound healing via modulation of inflammation and transforming growth factor beta 3

Abstract

To evaluate the effects and mechanisms of andiroba-based emulsion (ABE) topical treatment on full-thickness cutaneous wounds in rats. Methods: The wounds were harvested on days 3, 7, 15, and 20 post-surgery. Wound contraction rate, quantitative immunohistochemistry [macrophages, myofibroblasts, capillaries, collagens (col) I and III, transforming growth factor β3β (TGFβ3)], and tensile strength were assessed. Results: Treated wounds were smaller, contracted earlier and had increased angiogenesis, fewer CD68+ and M2 macrophages on days 7 and 15, but higher on day 20. Myofibroblasts appeared on days 3 to 7 in untreated wounds and on days 7 to 15 in treated wounds. TGFβ3 levels were higher in the treated wounds, less dense collagen fibers, lower col I/III ratios and a higher tensile strength. Conclusion: These results demonstrate the important anti-inflammatory role of treatment and the associated modulation of macrophages, myofibroblasts, and TGFβ3 levels. Collagen fibers in the treated wounds were more organized and less dense, similar to unwounded skin, which likely contributed to the higher tensile strength.

Development and Characterization of Squalene-Loaded Topical Agar-Based Emulgel Scaffold: Wound Healing Potential in Full-Thickness Burn Model

“…In accordance with these results, during the early stage of the wound healing process, when compared with the negative control and agar gels, the squalene-loaded emulgel scaffold-treated burnt wound injuries show a high influx of macrophages, and later during the maturation stage of wound healing, macrophage count was decreased due to the anti-inflammatory activity of the squalene. 27,[48][49][50][51] In the current research, when compared with the agar gels and negative control, the squalene-loaded emulgel scaffold endorsed a solid rise in Ki-67 cells. Moreover, this Ki-67 immunohistostaining was essential for identification of granular tissue formation and neovascularization during the burn wound-healing process.…”

“…Eventually, after rinsing under tap water, all the slides, 4th, 8th, 12th day tissue sections, were counter-stained with Harris' hematoxylin, dehydrated in ethanol, and finally were mounted with media. 27,[44][45][46][47][48][49][50][51]…”

O extrato aquoso da casca de Caryocar brasiliense melhora a retração de feridas

“…Resultados similares foram obtidos em estudos com uso de outras plantas. Chia et al (37) demonstraram que o uso de emulsão à base de andiroba em lesões produzidas experimentalmente em ratos, melhorou os níveis de fibroblastos e colágeno na lesão e Zain et al (30) observaram que o extrato de óleo de palma estimulou a proliferação e migração de fibroblastos em cultura de células. Tabela 1.…”

The chromatographic constitution of andiroba oil and his healing effects, compared to the LLLT outcomes, in oral mucositis induced in golden Syrian hamsters: a new treatment option

“…Studies have shown that essential fatty acids are the catalyzing agents of the healing process [38], stimulating cell proliferation, collagen production, antimicrobial, anti-inflammatory and antioxidant action [41][42][43][44][45][46][47][48][49]. The andiroba's oil shows many therapeutic finalities, due its natural repellent [49], dermatological properties [50,51], as well as its excellent anti-inflammatory, antibacterial performance, wound healing activity [52,53], antiparasitic, insecticide [54,55], anti-allergic effect [56], and antinociceptive responses [57,58].…”