Synergistic effect of honey and Nigella sativa on wound healing in rats

Abstract

1 AbstractPurpose: To investigate the therapeutic potential of honey, Nigella sativa (N. sativa) and their combination in rat model of excisional wound healing. Methods: A circular excision wound was established in the back region of 50 Wistar rats. Subsequently, they were divided into 5 groups and daily topical administration of lanolin in the control group, honey in the honey group, cold-pressed N. sativa seed oil in the N. sativa groups, mix of 1:1 ratio of honey and N. sativa seed oil in the mix group, and phenytoin cream in the phenytoin group were used. Then, wound surface areas were evaluated using digital camera immediately after the injury and at post excision days 5, 10, 15 and 20. Results: Significant reduction in wound surface area was observed within and between the groups (P < 0.001). In the post excision days 5, 10, 15 and 20 the wound surface areas in the mix group were significantly lower than the other groups followed by the phenytoin, honey, N. sativa, and control groups. Conclusion:The wound healing may be improved and accelerated by using topical solutions of honey, N. sativa seed oil and especially their mixture.

Herbal Products in Postsurgical Wound Healing – Incision, Excision and Dead Space Wound Models

“…Data from the articles cited in the present review referred to a total of 79 plants and showed that most of these plants exerted a beneficial effect on skin wound healing. Among these herbal products, A. vera gel [16,38,55,80], C. longa fresh paste/ethanolic extract [22,23,55], and Nigella sativa seed oil and powder [59][60][61] were the most examined. A. vera gel showed significant wound healing activity in comparison to tetracycline ointment [38], soframycin ointment [55], and povidone-iodine [16] via its antibacterial mechanism of action [55].…”

“…Fresh C. longa paste [22], polyherbal formulation containing C. longa extract [23], and lotion containing its ethanolic extract [55] increased wound breaking strength, significantly reduced epithelialization, and completed the wound healing process in 15 days. N. sativa oil [59], powder [60], and cream with extract [61] increased epithelialization and granulation as well as demonstrated significant wound contraction rate via its antioxidant activity. It is worth mentioning that most analyzed studies showed the potential of the examined plants in wound healing treatment.…”

Testimony for veterinary apitherapy

“…Topical honey application has a beneficial effect on the healing of cutaneous wounds, 130 ulcers 131 or infected chronic wounds showing synergism with Nigilla sativa EO. 132 There is good evidence for honey also having bioactivities that stimulate the immune response promoting tissue growth and wound repair, moreover suppress inflammation. 133 Twenty lactating cows suffering from claw lesions either digital dermatitis or interdigital necrobacillosis (infected wound in highly infected media) showing sever lameness were divided to equal four groups of five cows of each.…”

Antibacterial efficacy of black seed honey in combination with penicillin and amoxiclav against gram-positive bacteria

“…4 Honey carries the combination of organic acids (gluconic acid), phenolic acid, flavonoids, enzymes (invertase, diastase, glucose oxidase) and vitamins (thiamine B, B2 complex and B6) which facilitates collagen structure and fibroblast deposition during wound healing. 3,5 Many researchers have found its pharmacological properties (antiinflammatory, anti-oxidant, anti-cancer, anti-parasitic, immune stimulation etc.) by investigating its active components through in vivo and in vitro studies.…”