Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol

Abstract

This study aimed to evaluate the effect of using different concentrations (5, 7.5, 10, and 12.5%) of two permeating cryoprotectants, dimethyl sulphoxide (DMSO) and methanol, on Prochilodus lineatus embryos while being subjected to cooling for 2, 4, 6, and 8 h at 4 °C. We analyzed the hatching rate, viable larvae, and counts of hatched and spoiled eggs and those that did not complete their development after cooling. We observed a significant interaction among variables, permeable cryoprotectant concentration, and cooling time, having the increase of these factors caused a reduction in hatching rate. The curimba embryos showed a higher sensitivity to cold at a temperature of 4 °C for 6 and 8 h, directly influencing the production of viable larvae. We did not observe a significant difference in the variables analyzed when comparing the cryoprotectant solutions containing DMSO, methanol, and solutions containing only water and sucrose (0.5M). An increase in the concentration of cryoprotectant and cooling time promotes a reduction in the hatching rate and in the percentage of viable curimba larvae.

Characterization of short‐term storage of the semen ofTrachelyopterus galeatus(Siluriformes: Auchenipteridae) incorporating dimethylsulphoxide (dmso) to the diluent

“…Studies with fish have demonstrated the positive effects of refrigeration associated with the use of cryoprotectants among which stands out dimethylsulphoxide (DMSO), which is widely used for cooling embryos of Prochilodus lineatus , promoting conservation and raising the hatching rate of larvae (Machado et al, 2019) at low concentrations. Dimethylsulphoxide (DMSO) also prolonged the motility rate up to 13 days of refrigeration, increasing the fertilization capacity of semen from Oncorhynchus mykiss (Ubilla et al, 2015).…”

Sperm cryopreservation of Prochilodus lineatus throughout the same reproductive season

“…The streaked prochilod Prochilodus lineatus (Prochilodontidae, Characiformes) is a migratory neotropical fish with a large distribution in the main hydrographic basins of South America (Castro & Vari, 2004). This species is well adapted to captivity and has been used in reproduction studies (Machado et al, 2019; Miliorini et al, 2011; Paula et al, 2019), proving it can be used as a model species for the study of other neotropical fish species, which are considered endangered. The annual migration and spawn of the P. lineatus occur during the rainy months when water levels rise, water temperatures are higher, and the day length is longer (Vazzoler et al, 1997).…”

Use of melatonin as an inhibitor of apoptotic process for cryopreservation of zebrafish (Danio rerio) embryos

“…Introduction Development of effective techniques for cryopreservation of fish eggs and embryos could provide a valuable tool for the propagation of economically important and endangered species alike. The access to cryopreserved embryos in periods when natural spawning does not occur, could allow an increase in commercial production and conservation of rheophilic (Bozkurt, 2018;Machado et al, 2019) or any species of fish.…”