Feed additives can differentially modulate NF-κB (RelA/p65), IGF-1, GLUT2, and SGLT1 gene expression in porcine jejunal explants

Silveira, Hebert; Melo, Antônio Diego Brandão; Bortoluzzi, Cristiano; Costa, Leandro Batista; Rostagno, Marcos H.; Schinckel, A. P.; Garbossa, César Augusto Pospissil; Cantarelli, Vinícius de Souza

doi:10.1590/rbz4720180105

Cited by 3 publications

(5 citation statements)

References 40 publications

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“…The results demonstrated that A. oryzae, S. cerevisiae, or the combination of two probiotics up to 200 mg/kg in quails' diet had no significant impact on the physiological state, and the supplemental probiotics did not affect the ability of cholesterol transport from peripheral tissues to the liver. Regarding the effects of dietary S. cerevisiae on gene expression of SGLT1, these results are consistent with a previous report of Silveira et al (2018), who stated that the yeast culture increased the gene expression of SGLT1 in porcine jejunal explants. Similarly, Buts et al (1999) reported that the administration of S. boulardii by orogastric intubation at the level of 1 mg/g body weight per day for seven days increased twofold, the expression of SGLT1 in brush border membranes of resected rats.…”

Section: Discussionsupporting

confidence: 92%

“…The uptake of nutrients from lumen into the intestinal epithelial cells is regulated by nutrient transporters (Kaminski and Wong 2018). It has been reported that the probiotic supplementation affected the gene expression of some nutrient transporters in the enterocytes (Buts et al 1999;Silveira et al 2018). Among these transporters, the sodium-glucose cotransporter (SGLT1) is the main glucose transporter in the brush border membrane (Wright 2013).…”

Section: Introductionmentioning

confidence: 99%

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A comparison of two probiotics Aspergillus oryzae and, Saccharomyces cerevisiae on productive performance, egg quality, small intestinal morphology, and gene expression in laying Japanese quail

Zamanizadeh¹,

Mirakzehi²,

Agah

et al. 2021

Italian Journal of Animal Science

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This experiment was conducted to evaluate and compare the effects of a dietary inclusion with Aspergillus oryzae and Saccharomyces cerevisiae on productive performance, egg quality, intestinal morphology, and SGLT1 gene expression in laying Japanese quail. A total of 320 laying quails aged 47-day-old were randomly allocated to 8 treatments with 4 replicates of 10 quails each, for 7 weeks. The experimental diets consisted of a basal corn-soybean meal or control, control diet þ 50 mg/kg tetracycline, control diet þ 100 and 200 mg/kg A. oryzae, control diet þ 100 and 200 mg/kg S. cerevisiae, control diet þ 100 and 200 mg/kg of the combination of both A. oryzae and S. cerevisiae at equal proportions. At the end of the experiment, 4 quails per treatment were slaughtered, and tissue samples from the jejunum (1.5 cm in length) were removed for intestinal morphology measurements. Samples from the midsegment of the jejunum (150-200 mg) were taken to evaluate the gene expression of sodium-glucose cotransporter (SGLT1). The dietary inclusion of S. cerevisiae at 200 mg/kg significantly improved the feed conversion ratio (FCR) and egg production and reduced the feed intake (FI) (p < .001). The greatest shell thickness values were found in those fed on diets containing S. cerevisiae at 200 mg/kg. The quails fed on diets containing the combination of both probiotics at 100 mg/kg showed significantly higher egg weight (p < .001). Shell percent and yolk index increased significantly in quails fed on S. cerevisiae-supplemented diets at 100 mg/kg (p < .05). The villus height in the S. cerevisiae-supplemented group (200 mg/kg) was significantly higher than other groups (p < .05). The villus width was significantly higher in quails given 200 mg/kg A. oryzae compared to those received 100 mg/kg A. oryzae or 200 mg/kg S. cerevisiae (p < .05). Supplementation of S. cerevisiae at both tested levels resulted in significantly higher SGLT1 gene expression in the jejunum (p < .05). In conclusion, the results of this experiment demonstrated that among the two probiotics, S. cerevisiae at the level of 200 mg/kg as a functional feed additive has a better potential to improve productive performance, intestinal morphology, and SGLT1 gene expression of laying Japanese quails. HIGHLIGHTS Supplementation of diet with 200 mg/kg S. cerevisiae improved egg production, shell thickness, and FCR. The addition of 200 mg/kg S. cerevisiae to the diets improved the gut development by enhancing the villus height. Inclusion of diet with S. cerevisiae at both levels upregulated the gene expression of SGLT1.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

A comparison of two probiotics Aspergillus oryzae and, Saccharomyces cerevisiae on productive performance, egg quality, small intestinal morphology, and gene expression in laying Japanese quail

Zamanizadeh¹,

Mirakzehi²,

Agah

et al. 2021

Italian Journal of Animal Science

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“…In our study, IVE and PGO were found to promote the expression of SGLT1 in small intestinal villi of weaned piglets. Similar conclusions were confirmed in the study of Silveira et al, (2018) [40]. The difference of strain composition is an important reason for the inconsistent expression of SGLT1.…”

Section: Localization and Expressionsupporting

confidence: 85%

“…A total volume of 20 µL of the PCR mixture and reagents were added in accordance with the fluorescent quantitative PCR kit instructions. The optimized qRT-PCR protocol included an initial denaturation step at 95 • C for 30 s, followed by 40…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

The Combination of Plant Extracts and Probiotics Improved Jejunal Barrier and Absorption Capacity of Weaned Piglets

Yang

et al. 2022

Agriculture

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Plant extracts and probiotics play a vital role in maintaining animal intestinal health. However, their joint compatibility program still needs to be further explored. In our study, thirty two piglets (Duroc × Landrace × Yorkshire) were selected to divided into four treatments, which included basal diet, basal diet + 1000 mg/kg probiotics with added glucose oxidase (PGO), basal diet + 500 mg/kg Illicium verum extracts (IVE), and basal diet + 500 mg/kg IVE + 1000 mg/kg PGO. All the piglets were housed individually for the 42-d trial period after 7-d adaptation. Results showed that dietary supplementation of PGO and IVE increased the digestibility of ether extract (EE), crude protein (CP), and lysine (p < 0.05). Likewise, the net protein utilization (NPU) was also improved (p < 0.05). What is more, adding PGO and IVE reduced crypt depth, increased villus length, and chrionic gland ratio of piglets (p < 0.05). Additionally, IVE or PGO that was applied alone can increase the expression of Occludin, Zona occludens 1 (ZO-1), and Sodium-dependent glucose transporters 1 (SGLT1) in jejunum (p < 0.05). Our results strongly suggest that the combination of IVE and PGO can improve the nutrient digestibility of weaned piglets by increasing the expression of nutrient transport vectors (SGLT1 and CAT1) and tight junction proteins (Occludin and ZO-1) in the jejunum. In conclusion, the combination of plant extracts and probiotics is a vital strategy to improve animal health before the advent of antibiotic substitutes with absolute advantages.

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“…The gene expression modulation by water acidification promotes metabolic suppression, reduced protein synthesis and respiratory stress, and reduced metabolic scope, causing pathogen invasion, disease transmission, and host susceptibility (Chang et al, 2016;Wang et al, 2016). On the other hand, the energy destined to transcriptional response modulation can reduce shrimp muscular growth and productive performance (Silveira et al, 2018). Therefore, further long-term studies are necessary to determine how non-lethal high CO 2 concentrations influence growth, tissue histology, nutrient absorption, and physiologic response of shrimp cultured in RAS.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional response of immune-related genes in Litopenaeus vannamei cultured in recirculating aquaculture systems with elevated CO2

Casillas-Hernández¹,

Arévalo‐Sainz²,

Flores‐Pérez³

et al. 2021

Revista Brasileira De Zootecnia

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This short-term study evaluated the effect of non-lethal high CO 2 concentration on the transcriptional response of immune-related genes of Pacific white shrimp (Litopenaeus vannamei) cultured in recirculating aquaculture systems (RAS). Two experimental groups were created: high CO 2 (47.67±2.04 mg L −1 ) and low CO 2 (2.0±1.93 mg L −1 ). Shrimp of 8.85±1.20 g were placed randomly at a density equivalent to 100 individuals m −3 and were monitored at 6, 12, 18, and 24 h. The transcriptional response of immune-related genes was analyzed by qPCR. Gene expression of hemocyanin, prophenoloxidase, and heat shock protein 60 was downregulated at 24 h, suggesting affectations on oxygen transportation, melanization, and protein functioning of L. vannamei under high CO 2 concentrations. Also, gene up-regulation of lipopolysaccharide-and β-glucan-binding protein and cytosolic manganese superoxide dismutase can impair the bacterial recognition and antioxidant defense of shrimp exposed to high CO 2 concentrations. These results suggest that concentration at about 47 mg L −1 of CO 2 can significantly influence the transcriptional response modulation of immune-related genes.

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Feed additives can differentially modulate NF-κB (RelA/p65), IGF-1, GLUT2, and SGLT1 gene expression in porcine jejunal explants

Cited by 3 publications

References 40 publications

A comparison of two probiotics Aspergillus oryzae and, Saccharomyces cerevisiae on productive performance, egg quality, small intestinal morphology, and gene expression in laying Japanese quail

A comparison of two probiotics Aspergillus oryzae and, Saccharomyces cerevisiae on productive performance, egg quality, small intestinal morphology, and gene expression in laying Japanese quail

The Combination of Plant Extracts and Probiotics Improved Jejunal Barrier and Absorption Capacity of Weaned Piglets

Transcriptional response of immune-related genes in Litopenaeus vannamei cultured in recirculating aquaculture systems with elevated CO2

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