Transcriptional response of immune-related genes in Litopenaeus vannamei cultured in recirculating aquaculture systems with elevated CO2

Casillas-Hernández, Ramón; Arévalo‐Sainz, Karla Janeth; Flores‐Pérez, María Belem; Rodriguez-Anaya, Libia Zulema; Lares‐Villa, Fernando; Bórquez-López, Rafael Apolinar; Gil‐Núñez, Juan Carlos; Gonzalez-Galaviz, Jose Reyes

doi:10.37496/rbz5020200197

Cited by 2 publications

(2 citation statements)

References 37 publications

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“…High‐quality total RNA from RAW 264.7 cells was extracted with Trizol® Reagent (Life Technologies, CA, USA), in agreement with the manufacturer's instructions. Parameters and conditions of the qPCR analysis were followed as described in Casillas‐Hernández et al (2021). Determination of the levels of gene‐relative expression was achieved with the following equation (Livak & Schmittgen, 2001).…”

Section: Methodsmentioning

confidence: 99%

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Antioxidant potential, cytokines regulation, and inflammation‐related genes expression of phenolic extracts from Mexican oregano

Leyva‐López

Gutiérrez‐Grijalva

Heredia

et al. 2022

Journal of Food Biochemistry

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The Mexican population traditionally uses oregano infusions to treat oxidative and inflammation‐related disorders. Therefore, this study was focused on the examination of the antioxidant capacity and potential against inflammation from three Mexican oregano species (Lippia graveolens [LG], Lippia palmeri [LP], and Hedeoma patens [HP]). The extracts from LG showed a superior total phenolic content. LG, LP, and HP exhibited a higher capacity to inhibit the radical DPPH (up to 90.33 ± 0.25%) and significantly lowered the release of MCP‐1 and IL‐6. At the same time, LG and HP increased the secretion of IL‐10. Extracts from LG, LP, and HP did not significantly diminish the expression of il‐1β or inos, although a slight decrease in inos expression was observed. Our findings support that phenolic extracts from L. graveolens, L. palmeri, and H. patens possess antioxidant and anti‐inflammatory properties and might be potential therapeutic candidates against oxidative and inflammation‐related diseases. Practical applications Oregano species have traditionally been exploited as remedies against inflammatory‐related diseases, namely headaches, asthma, bowel disorders, and rheumatism. This study explored the antioxidant potential of three Mexican oregano species (Lippia graveolens, Lippia palmeri, and Hedeoma patens) and their anti‐inflammatory effects in a murine cell model. Phenolic extracts from oregano showed antioxidant capacity and exerted activity against inflammation by improving anti‐inflammatory cytokines secretion or negatively regulating pro‐inflammatory cytokines. The results of our study demonstrate that the phenolic extracts from these Mexican oregano species have the potential in treating inflammation‐related diseases.

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Section: Methodsmentioning

confidence: 99%

“…Parameters and conditions of the qPCR analysis were followed as described in Casillas-Hernández et al (2021). Determination of the levels of gene-relative expression was achieved with the following equation (Livak & Schmittgen, 2001).…”

Section: Analysis Of Inflammatory-related Genes Expression By Qpcrmentioning

confidence: 99%

Antioxidant potential, cytokines regulation, and inflammation‐related genes expression of phenolic extracts from Mexican oregano

Leyva‐López

Gutiérrez‐Grijalva

Heredia

et al. 2022

Journal of Food Biochemistry

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Dietary Use of Methionine Sources and Bacillus amyloliquefaciens CECT 5940 Influences Growth Performance, Hepatopancreatic Histology, Digestion, Immunity, and Digestive Microbiota of Litopenaeus vannamei Fed Reduced Fishmeal Diets

Casillas-Hernández

Gonzalez-Galaviz

Rodriguez-Anaya

et al. 2022

Animals

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An 8-week feeding trial investigated the effect of Fishmeal (FM) replacement by soybean meal (SBM) and poultry by-product meal (PBM) in diets supplemented with DL-Met, MET-MET (AQUAVI®), Bacillus amyloliquefaciens CECT 5940 (ECOBIOL®) and their combinations on growth performance and health of juvenile Litopenaeus vannamei. A total of six experimental diets were formulated according to L. vannamei nutritional requirements. A total of 480 shrimp (0.30 ± 0.04 g) were randomly distributed into 24 tanks (4 repetitions/each diet, 20 shrimp/tank). Shrimp were fed with control diet (CD; 200 g/Kg fishmeal) and five diets with 50% FM replacement supplemented with different methionine sources, probiotic (B. amyloliquefaciens CECT 5940) and their combinations: D1 (0.13% DL-MET), D2 (0.06% MET-MET), D3 (0.19% MET-MET), D4 (0.13% DL-MET plus 0.10% B. amyloliquefaciens CECT 5940 and D5 (0.06% MET-MET plus 0.10% B. amyloliquefaciens CECT 5940). Shrimp fed D3 and D5 had significantly higher final, weekly weight gain, and final biomass compared to shrimp fed CD (p < 0.05). Shrimp fed D2 to D5 increased the hepatopancreas epithelial cell height (p < 0.05). Digestive enzymatic activities were significantly increased in shrimp hepatopancreas’ fed D3 (p < 0.05). Meanwhile, shrimp fed D1 had significant downregulation of immune-related genes (p < 0.05). Moreover, shrimp fed D3 and D5 increased the abundance of beneficial prokaryotic microorganisms such as Pseudoalteromonas and Demequina related to carbohydrate metabolism and immune stimulation. Also, shrimp fed D3 and D5 increased the abundance of beneficial eukaryotic microorganism as Aurantiochytrium and Aplanochytrium were related to eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) production which plays a role in growth promoting or boosting the immunity of aquatic organisms. Therefore, fishmeal could be partially substituted up to 50% by SBM and PBM in diets supplemented with 0.19% MET-MET (AQUAVI®) or 0.06% MET-MET (AQUAVI®) plus 0.10% B. amyloliquefaciens CECT 5940 (ECOBIOL®) and improve the productive performance, health, and immunity of white shrimp. Further research is necessary to investigate synergistic effects of amino acids and probiotics in farmed shrimp diets, as well as to evaluate how SBM and PBM influence the fatty acid composition of reduced fishmeal diets and shrimp muscle quality. Nevertheless, this information could be interesting to develop low fishmeal feeds for aquaculture without affecting the growth and welfare of aquatic organisms.

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Transcriptional response of immune-related genes in Litopenaeus vannamei cultured in recirculating aquaculture systems with elevated CO2

Cited by 2 publications

References 37 publications

Antioxidant potential, cytokines regulation, and inflammation‐related genes expression of phenolic extracts from Mexican oregano

Antioxidant potential, cytokines regulation, and inflammation‐related genes expression of phenolic extracts from Mexican oregano

Dietary Use of Methionine Sources and Bacillus amyloliquefaciens CECT 5940 Influences Growth Performance, Hepatopancreatic Histology, Digestion, Immunity, and Digestive Microbiota of Litopenaeus vannamei Fed Reduced Fishmeal Diets

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