In vitro study of postbiotics from Lactobacillus plantarum RG14 on rumen fermentation and microbial population

Izuddin, Wan Ibrahim; Loh, Teck Chwen; Samsudin, Anjas Asmara; Foo, Hooi Ling

doi:10.1590/rbz4720170255

Cited by 32 publications

(27 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Briefly, twelve male lambs (17.3 ± 0.58 kg, 16 weeks old) were randomly divided to the control group (no postbiotic inclusion) and postbiotic group (0.9% (v/w) postbiotic RG14 inclusion). The postbiotic RG14 was chosen based on the highest antioxidant activity by ABTS and DPPH assay and a 0.9% level of postbiotic RG14 inclusion was selected according to in vitro findings reported by Izuddin, et al [10]. The lambs were individually penned and fed the treatment diets for 60 days including the adaptation period for 14 days.…”

Section: Feeding Trial and Sample Collectionmentioning

confidence: 99%

“…In broilers exposed to heat stress, postbiotics inclusion was shown to alleviate the heat stress effects by the greater growth performance, immune status and gut health [9]. Postbiotics use in ruminants was shown to improve the in vitro rumen fermentation and fiber degrading microbial population [10] and enhance growth performance, nutrient utilization, immune status and gut health in post-weaning lambs [11,12].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dietary Postbiotic Lactobacillus plantarum Improves Serum and Ruminal Antioxidant Activity and Upregulates Hepatic Antioxidant Enzymes and Ruminal Barrier Function in Post-Weaning Lambs

et al. 2020

Self Cite

View full text Add to dashboard Cite

Postbiotics from Lactobacillus plantarum have been reported to improve growth performance, nutrient utilization, immune status and gut health in livestock. However, there is scarce information on the antioxidant activity of postbiotics and its modulation of antioxidant activity and rumen barrier function in animals. We investigated the antioxidant activity of postbiotics from L. plantarum RG14, RG11 and TL1 and dietary effects in post-weaning lambs on serum and ruminal antioxidant activity, hepatic antioxidant enzymes and ruminal barrier function. Postbiotic RG14 showed the highest antioxidant activity in both 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay and was chosen to be evaluated in animal trials. Twelve post-weaning Dorper lambs were allotted to the control group and postbiotic group (0.9% (v/w) postbiotic RG14). The improvement in antioxidant activity of the postbiotic group was observed by greater glutathione peroxidase (GPX) in serum and ruminal fluid and lower serum TBARS. The findings were strengthened by the upregulation of hepatic GPX1, GPX4 and copper, zinc superoxide dismutase (Cu/Zn SOD) in the postbiotic group. Lambs received postbiotics had higher regulation of rumen barrier function through upregulation of tight junction protein (TJP), occludin (OCLD), claudin-1 (CLDN1) and CLDN4. The current study demonstrated that dietary postbiotics enhanced the serum and ruminal fluid antioxidant activity, reduced the serum lipid peroxidation and upregulated hepatic antioxidant enzymes and ruminal barrier function.

show abstract

Section: Feeding Trial and Sample Collectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dietary Postbiotic Lactobacillus plantarum Improves Serum and Ruminal Antioxidant Activity and Upregulates Hepatic Antioxidant Enzymes and Ruminal Barrier Function in Post-Weaning Lambs

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Probiotics for adult ruminants have mainly been selected to improve fibre digestion by rumen micro-organisms (Uyeno et al 2015). Furthermore, it has been suggested that the influence of Lactobacillus during digestion is dependent on dosage or level (Jiao et al 2017;Izuddin et al 2018).…”

Section: Ruminal Gas Kinetics and Total Cumulative Productionmentioning

confidence: 99%

“…Lactobacillus sp., Weissella , Aerococcus , Bifidobacterium and Enterococcus have been used as probiotics with beneficial effects on the host (Uyeno et al ). The effects could be in the form of microbial stability, improving digestibility, reducing or preventing establishment of pathogens, preventing acidosis and enhancing the growth of beneficial microflora population (Izuddin et al ). Lactic acid bacteria (LB) ferment carbohydrate to produce lactic acid (Astuti et al ).…”

Section: Introductionmentioning

confidence: 99%

Role of dose‐dependent Lactobacillus farciminis on ruminal microflora biogases and fermentation activities of three silage‐based rations

et al. 2019

View full text Add to dashboard Cite

Aims The influence of Lactobacillus farciminis on ruminal fermentation characteristics was elucidated in this study. Methods and Results Ruminal fermentation was conducted using maize silage ration (R) and concentrate (C) as 75R:25C, 50R:50C and 25R:75C, supplemented with lactic acid bacteria (LB) at 0, 20 and 30 mg g−1 dry matter substrate and their interaction (1st experiment). The same LB product was used at 0, 20, 40 and 60 mg g−1 dry matter of the mixture (1 : 1) of oat straw and concentrate for 48 h of incubation (2nd experiment). At 24 and 48 h of incubation, LB0 produced the highest biogas and LB20 produced the lowest, whereas at 48 h of incubation LB40 produced the lowest. In ration x LB, LB40 resulted in the highest biogas production, while LB0 had the lowest (P < 0·001) at 8, 10 and 12 h of incubation. Inclusions of LB0, 20, 40 and 60 mg g−1 dry matter resulted in a linear increase (P < 0·003) in the asymptotic biogas production and fermentation parameters in a dose‐dependent manner, except in pH which decreased (P = 0·029). Conclusions The use of L. farciminis in diet with high level of concentrate without any adverse effect on the pH of rumen fluid to the point of acidosis. Furthermore, in high forage diet, the use of L. farciminis would help to improve the ruminal fermentation digestibility and mitigate ruminal biogas production. Significance and Impact of the Study Using Lactobacillus as a feed additive can improve ruminal fermentation activities by maintaining the stability of pH in the rumen and improving the feed utilization through manipulation of the microbial ecosystem.

show abstract

“…It has recently been observed that the dietary supplementation of postbiotics promoted the health and growth performance in broilers [31][32][33], layers [34,35] and piglets [36,37]. More recently, postbiotics have been reported enhancing the growth performance, rumen fermentation, immune status, antioxidant defence system, and gut health in small ruminants [38][39][40]. Under normal environmental temperature, dietary supplementation of postbiotics improved health and growth performance of broilers by promoting their immune status, growth genes expression and gut health as their supplementation significantly improved the intestinal villus, decreased the population of Enterobacteriaceae and faecal pH, and increased the population of lactic acid bacteria [25,[31][32][33]41].…”

Section: Introductionmentioning

confidence: 99%

Supplementation of Postbiotic RI11 Improves Antioxidant Enzymes Activity, Upregulated Gut Barrier Genes and Reduced Cytokines, Acute Phase Proteins and HSP70 Gene Expression Levels in Heat-Stressed Broilers

Humam

Loh

Foo

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background To alleviate the adverse impacts of stressful environmental conditions on poultry and promoting the animal's health and growth performance, antibiotics have been added to poultry diets as growth promoters. Nevertheless, improper and overuse of antibiotics as feed additives have resulted the emergence of antibiotic-resistant bacteria and increased the levels of antibiotic residues in animal products, which have disastrous effects on the health of both animals and humans. Postbiotics produced from probiotic Lactobacillus plantarum have been the recent research of interest as dietary additives for livestock and potential alternatives to antibiotics. However, there is very scarce of study has considered the effect of postbiotics on broilers under heat stress. The aim of this work was to evaluate the impacts of feeding different levels of postbiotic RI11 on antioxidant enzyme activity, physiological stress indicators, cytokines and gut barrier genes expression in broilers under heat stress. Materials and Methods A total of 252 male Cobb 500 were fed with 1 of 7 diets: NC (negative control, 0.0% RI11) basal diet; OTC (positive control) NC + 0.02% (w/w) oxytetracycline; AA (antioxidant control) NC + 0.02% (w/w) ascorbic acid. Four further groups were NC + 0.2, 0.4, 0.6 and 0.8% postbiotic RI11 (v/w) of the respective levels. Results Supplementation of different levels of postbiotic RI11 increased plasma glutathione peroxidase, catalase and glutathione enzymes activity. Postbiotic RI11 groups upregulated the mRNA expression of interleukin 10 and downregulated of interleukin 8, tumor necrosis factor-alpha, heat shock protein 70 and alpha 1- acid glycoprotein levels compared to the NC and OTC groups. Feeding various doges of postbiotic RI11 improved the integrity of the intestinal barrier by the upregulation of zonula occludens-1 and mucin2 mRNA expressions. However, no difference was observed in claudin1, ceruloplasmin, interleukin 6, interleukin 2 and interferon expression, but downregulation for occludin expression as compared with the NC group. Supplementation of postbiotic RI11 in different levels quadratically increased the plasma glutathione peroxidase, catalase and glutathione, interleukin 10, mucin2 and zonula occludens-1 mRNA expression, and reduced plasma interleukin 8, tumor necrosis factor-alpha, alpha 1- acid glycoprotein and heat shock protein 70 mRNA expression. Supplementation of postbiotic RI11 at level 0.6% was sufficient to achieve the improvement in health of broiler chickens under heat stress as compared to other levels. Conclusions The results suggested that postbiotic produced from L. plantarum RI11 particularly at level 0.6% (v/w) could be used as an alternative to antibiotics and natural sources of antioxidants in the poultry industry.

show abstract

In vitro study of postbiotics from Lactobacillus plantarum RG14 on rumen fermentation and microbial population

Cited by 32 publications

References 32 publications

Dietary Postbiotic Lactobacillus plantarum Improves Serum and Ruminal Antioxidant Activity and Upregulates Hepatic Antioxidant Enzymes and Ruminal Barrier Function in Post-Weaning Lambs

Dietary Postbiotic Lactobacillus plantarum Improves Serum and Ruminal Antioxidant Activity and Upregulates Hepatic Antioxidant Enzymes and Ruminal Barrier Function in Post-Weaning Lambs

Role of dose‐dependent Lactobacillus farciminis on ruminal microflora biogases and fermentation activities of three silage‐based rations

Supplementation of Postbiotic RI11 Improves Antioxidant Enzymes Activity, Upregulated Gut Barrier Genes and Reduced Cytokines, Acute Phase Proteins and HSP70 Gene Expression Levels in Heat-Stressed Broilers

Contact Info

Product

Resources

About