In vitro anticancer potentials of Lactobacillus plantarum IIA-1A5 and Lactobacillus acidophilus IIA-2B4 extracts against WiDr human colon cancer cell line

Abstract

Colorectal cancer is an emerging public health problem in Indonesia that urges more serious attempts to combat the disease. Previous in vitro studies indicated some probiotics offer promising avenues for the prevention and treatment of colorectal cancer. Earlier, two Indonesian meat-based probiotics of Lactobacillus plantarum IIA-1A5 and L. acidophilus IIA-2B4, were isolated and exhibited promising functional properties in food fermentation. Nevertheless, their potential anticancer activity has never been examined. This study aims to determine the inhibitory properties of L. plantarum IIA-1A5 and L. acidophilus IIA-2B4 against the WiDr human colon carcinoma cell line. To address, extracellular and intracellular extracts were firstly harvested from both probiotics at the population of 8.22 and 9.13 log (cfu/mL) for L. plantarum IIA-1A5 and L. acidophilus IIA-2B4, respectively. The extracts from both bacteria were found to have similar pH and titratable acidity values, but different in their protein contents. Further, extra-and intracellular extracts of both strains displayed inhibitory activity towards WiDr cells in a dose-dependent response. The calculated IC50 values of extracellular extracts of both probiotics were significantly lower than that of the intracellular extracts. This activity is predicted to be partly contributed by some protein substances in the extract.

Fatty acid profiles of Lactobacillus plantarum IIA-1A5 and Lactobacillus acidophilus IIA-2B4

“…The probiotic cultures of L. plantarum IIA-1A5 and L. acidophilus IIA-2B4 were obtained from the IPB University Laboratory of Animal Product Technology collection. Culture propagation was done according to Adiyoga et al [7]. The cultures were inoculated in de Man, Rogosa, and Sharpe (MRS) broth, and incubated for 24 h at 37°C.…”

“…Bacterial extracts were harvested according to Adiyoga et al with modifications [7]. Briefly, probiotic cultures were subjected to centrifugation at 8,000 x g, 4˚C, for 15 min.…”

“…In this study, two indigenous LAB strains, Lactobacillus plantarum IIA-1A5 (NCBI accession number: OR473281) and Lactobacillus acidophilus IIA-2B4, known for their probiotic-like characteristics will be the main focus. These characteristics include: strong antimicrobial activity against pathogenic microorganisms, immunomodulatory activity, high tolerance against low pH values, and recently, in vitro anticancer activities against WiDr colon cancer cells have been identified [4,5,6,7].…”

A Novel Probiotic-Based Oral Vaccine against SARS-CoV-2 Omicron Variant B.1.1.529

“…They are regarded as Generally Recognized As Safe (GRAS) by the Food and Drug Administration (FDA) of the United States [ 10 ]. While consuming live microorganisms in sufficient quantities, they can survive in the host digestive system and produce several advantageous effects on the host, such as providing anti-colorectal cancer effects and preventing inflammatory bowel diseases [ 11 , 12 ], and are commonly referred to as probiotics [ 13 ]. In comparison to traditional injectable vaccines, the administration of probiotic-based mucosal vaccines is easier, safer, and cheaper than traditional injectable vaccines, making them advantageous for mass immunization campaigns, particularly in resource-limited countries [ 14 , 15 ].…”

A new anti-colon cancer tumor pathway of Phenyllactic acid by reducing adhesion of Fusobacterium nucleatum

“…Traditional treatments include aspirin and nitrozolazole (Brennan et al, 2021) which are treated CRC drug-resistant by inhibiting the Fn, but aspirin damages the gastrointestinal tract, and nitrozolazole causes diarrhea. Probiotic Treatment are attracting attention for their safe, natural and healthy features (Adiyoga et al, 2022;Al-Megrin et al, 2022). Phenyllactic acid (PLA) as a new organic acid metabolized by probiotic bacteria such as Lactobacillus plantarum (Sun et al, 2019b), which is a kind of small-molecule organic bacteriostatic substance, PLA has a stable structure and can withstand high temperatures.…”