Purification of polysaccharide from Solanum nigrum L. by S-8 macroporous resin adsorption

Abstract

Solanum nigrum medical slices, bought from Zhejiang Schwab Pharmaceutical co., LTD., was identified in accordance with Chinese pharmacopoeia (catalog number 20150720).Anhydrous glucose standard was from Chinese Medicine Shanghai Chemical Reagent Company. S-8, D3520, HPD-450 macroporous adsorption resin were from Cangzhou Baoen chemical co., LTD. Phenol, sodium hydroxide, hydrochloric acid, sulfuric acid, ethyl ether, anhydrous ethanol and other conventional reagents, are the domestic analytical pure. BS224S type electronic analytical balance was bought from Beijing Sartorius. JHBE-50T type extractors was from Henan Jinding technology). RV10-V type rotary evaporation instrument was from IKA Germany. SHZ-B type water-bathing constant temperature vibrator was from Shanghai Boxun Co. LTD. I3 type UV-VIS spectrophotometer was from Jinan Hanon Instrument Co. LTD. Experimental methods Preparation of solanum nigrum polysaccharide solutionSolanum nigrum medicine was smashed and sieved, adding 4 times the amount of petroleum ether, degreasing 2 h with 50 °C reflux for 2 times. Dried the residue, then placed in the flash type extractors, adding 30 times the amount of water, flash extraction with 80V extract voltage for 2 mins for 2 times. Merged the extracted liquid, filtration, concentrated to 1/2 amount of medicinal herbs. Then took supernatant after centrifugation, adding ethanol to the alcohol content of 80%. After refrigerated standing at 4 °C for one night, vacuum suction filter, precipitation with ethanol, ether in turn twice, vacuum drying at 60 °C, then the solanum nigrum polysaccharide was prepared. Took precise amount of solanum nigrum polysaccharide, adding water and dissolving under the condition of ultrasonic, a

Effective extraction and determination of 24 quinolones in water and egg samples using a novel magnetic covalent organic framework combined with UPLC-MS/MS

“…It is worth noting that previous reports have mostly focused on the detection of milk and meat samples with less detection in egg samples. [41][42][43]58,59 In reported methods, most of them involved the detection of 3-6 QNS. Only one method for 17 QNs detected in aquaculture wastewater was established, and the testing sample matrix was single.…”

“…39,40 To the best of our knowledge, there are few reports on the application of MCOF materials in the eld of adsorbing QNs. In the reports on the application of the MSPE method to determine QNs, 23,[41][42][43][44] there are few reports on methods that can simultaneously detect QNs in different matrices, and the number of QNs is no more than 14. Therefore, developing a unied method capable of simultaneously detecting large amounts of QNs residues in different matrices remains a challenge.…”

The effects of bioactive components in Solanum nigrum against oxidative stress in liver damage

“…S. nigrum consist of many constitutes like anthocyanins, anthocyadins, flavonoids, tanins, vitamin C, vitamin E, and small quantities of iron, zinc, selenium, gallic acid and quercetin which act as an active ingredient in controlling oxidation which results in the prevention of oxidative stress, acute liver toxicity and metabolic ailments such as diabetes, cardiovascular diseases and many types of cancer (Saibu et al, 2020). S. nigrum is one of the richest sources of anthocyanins after berries (Huang et al, 2022;Li et al, 2021a). Being antioxidants in nature, they donate the pair of electrons and stabilize the free radicals in body (Khan et al, 2021;Teng et al, 2022a).…”

The kinetics of heparin adsorption with Dowex 1x1 ion exchange resin

“…Generally, patent procedures differ mainly in the choice of extraction agent, the method of breaking the heparin protein complex, and the final purification method. The ion exchange resins were used for the isolation and purification of different products derived from plant or animal tissues, as it supports the reliable capture and removal of biologically active substances with a high molecular weight (Flengsrud et al, 2010;Huang et al, 2022;Pan et al, 2022). The different type of resins such as Dowex ® (Warda et al, 2003;Flengsrud et al, 2010), Amberlite (Vreeburg & Baauw, 2010), Lewatit (Linhardt et al, 1992), and Sephadex A-25 anion-exchange resin (Luppi et al, 2005) was designed and tested for heparin recovery.…”