Total flavonoids from Lagerstroemia speciosa (L.) Pers inhibits TNF-α-induced insulin resistance and inflammatory response in 3T3-L1 adipocytes via MAPK and NF-кB signaling pathways

Abstract

Adipocyte tissue inflammation orchestrates the occurrence and development of diseases induced by obesity. Here, the effects of Lagerstroemia speciosa (L.) Pers total flavonoids (LTF) on TNF-α-induced insulin resistance (IR) and inflammatory response in 3T3-L1 adipocytes and underlying mechanisms were studied. Cell glucose uptake test confirmed that TNF-α could induce IR in 3T3-L1 adipocytes. The sensitivity of 3T3-L1 adipocytes to insulin was increased by LTF treatment. The phosphorylation of IRS1 and Akt in TNF-α-treated 3T3-L1 adipocytes was markedly restored by LTF treatment. The results revealed that interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) mRNA expression was elevated in TNF-α-administered 3T3-L1 adipocytes, whereas the mRNA expression of adiponectin (ADPN) was considerably decreased. When treated with 50 μg/mL LTF, the IL-6 and MCP-1 mRNA expression levels were drastically reduced, and the ADPN mRNA expression level was significantly raised. Moreover, the concentration of IL-6 in the supernatant of the medium was decreased after LTF treatment compared with the model group. Western blotting results showed that the phosphorylation of p38, JNK, and ERK of mitogen-activated protein kinase (MAPK) signaling pathways and IкB of NF-кB signaling pathway were inhibited. It suggested that LTF improves TNF-α-induced IR and inflammatory response in 3T3-L1 adipocytes via inhibiting MAPK and NF-кB signaling pathways.

Physalis alkekengi L. Calyx Extract Alleviates Glycolipid Metabolic Disturbance and Inflammation by Modulating Gut Microbiota, Fecal Metabolites, and Glycolipid Metabolism Gene Expression in Obese Mice

“…Studies showed that obesity-induced proinflammatory cytokines were closely related to insulin resistance [58,59]. However, in insulin-resistant individuals, a large number of proinflammatory cytokines and inflammatory mediators, especially TNF-α, MCP-1, and IL-6, were upregulated [60]. The current research illustrated for the first time that PC could significantly inhibit HFHF-induced inflammatory response, which was manifested in the reduction of inflammatory factors (TNF-α, IL-1β, and MCP-1) in serum and liver tissues.…”

The impact of Apocynum venetum tea flavonoids on G422 glioma in vivo

“…Flavonoids are abundant in plant foods such as fruits, vegetables, tea leaves, and so on. Natural flavonoids have been proven in studies to be a class of chemicals with considerable anti-glioma efficacy, as well as low toxicity and side effects and excellent safety (Hardinasinta et al, 2022;Yin et al, 2022). Natural phytoflavonoids can suppress gliomas across the blood-brain barrier and can have a role in glioma resistance reversal.…”