Preparation of high fischer ratio oligopeptide of chlorella powder using specific enzymatic hydrolysis

Proteases: Importance, Immobilization Protocols, Potential of Activated Carbon as Support, and the Importance of Modifying Supports for Immobilization

“…Xiong et al [ 134 ] immobilized alkaline protease on activated carbon for the production of high Fischer ratio oligopeptides. Santos et al [ 64 ], immobilized trypsin on activated carbon subjected to different modifications (glutaraldehyde, genipin, and metallization) and demonstrated that trypsin immobilized on activated carbon modified with iron ions in the presence of a chelating agent presented better results in hydrolytic activity, in addition to presenting better operational stability when comparing with the soluble enzyme.…”