Near infrared spectroscopy (NIRS) combined with various chemometrics methods was tried to identify the fresh tea leaves at different altitudes quickly and nondestructively. Three kinds of samples were collected, then scanning NIRS, conducting spectral preprocessing to remove noise information, using backward interval partial least squares to screen characteristic spectral intervals, going on principal component analysis, respectively. Finally, least squares support vector machine method (LS-SVM) was applied to establish NIRS models, whose robustness was tested by prediction set samples. The best pretreated method was the combination of multivariate scattering correction and the first derivative. Six characteristic spectral intervals were screened, and the corresponding spectral wavenumbers were 4821.