Inhibitory effects of tanshinone IIA from Salvia miltiorrhiza Bge on human bladder cancer BIU-87 cells and xenograft in nude mice

Abstract

The objective of this study was to investigate the inhibitory effects of tanshinone OOA on human bladder cancer BOU-87 cells and the xenograft in nude mice. BOU-87 cells were treated with tanshinone OOA with concentration of 0.5, 1, 2 and 4 g/L. The cell proliferation, cycle and apoptosis were detected. The nude mice with BOU-87 cell xenograft were treated with normal saline (control), 5-fluorouracil (5-FU), 200, 400 and 600 mg/kg tanshinone OOA, respectively. The animal body weight and growth of tumor were measured. The expressions of B-cell lymphoma-2 (Bcl-2), Caspase-3 and proliferating cell nuclear antigen (PCNA) protein in xenograft were detected. The apoptosis index of cells was determined. Results showed that, tanshinone OOA inhibited the proliferation of BOU-87 cells, promoted their apoptosis, and arrested more cells in G 0 /G 1 phase. Tanshinone OOA significantly inhibited the growth of xenograft in nude mice and promoted the apoptosis of tumor cells in xenograft. Tanshinone OOA down-regulated Bcl-2 and PCNA expression and up-regulated Caspase-3 expression in xenograft. On conclusion, tanshinone OOA has inhibitory effects on human bladder cancer BOU-87 cells, and can inhibit the BOU-87 cells xenograft in nude mice.

[RETRACTED ARTICLE]Isolation of curcumol from zedoary turmeric oil and its inhibitory effect on growth of human hepatocellular carcinoma xenografts in nude mice

“…Studies have found that many plants contain the ingredients which have good activities in antioxidant, anti-inflammatory, anti-tumor and other aspects (Costa et al, 2020;Huang et al, 2020;Santos et al, 2021). Curcumol is a sesquiterpene compound isolated from the zedoary turmeric oil which has long been used as a folk medicine in China for many years (You et al, 2005).…”

5-Aminolevulinic acid could enhance the salinity tolerance by alleviating oxidative damages in Salvia miltiorrhiza

“…Tanshinone in S. miltiorrhiza has definite curative effect on cardiovascular diseases and neurological diseases (Hao et al, 2015;Xu et al, 2021). Recently, tanshinone has been found to have an inhibitory effect on human bladder cancer cells (Huang et al, 2020). In addition, Gengmao et al (2014) found that the adaptive response to salt stress in S. miltiorrhiza was linked with its highly induced antioxidant enzyme defense systems.…”

Effects of red meat diet on gut microbiota in mice

“…Moreover, Lachnospiraceae is a highly abundant bacterial family within the gut microbiota in human, whose members may protect against human colon cancer by producing butyric acid, a substance that is important for both microbial and host epithelial cell growth (Meehan & Beiko, 2014;Yang et al, 2020;Huang et al, 2020;Hwang et al, 2019;Grom et al, 2020). In this study, the compositions of Lachnospiraceae in the control, lowdose, medium-dose and high-dose groups were 34.1%, 36.9%, 32.84% and 31.39%, respectively.…”