Effect of dihydroferulic acid obtained from fermented rice bran extract on neuroprotection and behavioral recovery in an ischemic rat model

Abstract

This study aimed to evaluate whether dihydroferulic acid (dFA) promoted the viability of H 2 O 2-treated PC12 cells and functional recovery from ischemic injury. The animals were divided into four groups for the study: (1) the vehicle treated (saline, 1 mL/kg), (2) dFA 5 mg/kg treated, (3) dFA10 mg/kg treated, and (4) dFA 20 mg/kg treated groups. Neurological deficit was evaluated using the modified neurological severity score. Real-time polymerase chain reaction analyses were performed with the protein disulphide isomerase (PDI), nuclear factor-E2-related factor 2 (Nrf2), nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) genes. Immunohistochemical analysis was performed with the Iba-1 and MFG-E8 genes. dFA treatment improved the reduced viability of PC12 cells induced by H 2 O 2 in a dose-dependent manner. Only 50 μM of dFA significantly enhanced the transcription levels of antioxidant genes and neurotrophic factors compared to the vehicle group. In vivo dFA administration exerted a neuroprotective effect by reducing the infarct volume and enhancing behavioral function following cerebral ischemia. dFA treatment protected neuronal cells from ischemic injury and increased the transcription levels of anti-oxidant genes (PDI and Nrf2) and neurotrophic factors (BDNF and NGF). dFA treatment decreased the expression of Iba-1 and MFG-E8 genes, which signal neural cell death.

Phenolic compounds profile and antioxidant activity of pea (Pisum sativum L.) and black bean (Phaseolus vulgaris L.) sprouts

“…It also increases the content of ferulic acid in beans three times (10 d) and allows its synthesis in pea. Ferulic acid exerted a neuroprotective effect and enhancing behavioral function following cerebral ischemia (Lee et al, 2020).…”

Cryptotanshinone protects hippocampal neurons against oxygen-glucose deprivation-induced injury through the activation of Nrf2/HO-1 signaling pathway

“…After the onset of brain ischemia, a series of events lead ultimately to the death of neurons (Sutherland et al, 2012). Growing evidence has supported that anti-oxidative, anti-inflammatory and cell protective properties of natural plants and active constituents (Popović et al, 2010;Lee et al, 2010). However, therapies for ischemic brain injury are far from satisfactory and many of these attempts have failed.…”

Preparation of total saponins from Panax japonicus and their protective effects on learning and memory ability of aging mice

“…Studies have found that many plant extracts have good pharmacological effects (Lee et al, 2020;Wu et al, 2020;Santos et al, 2021). As TSPJ are the main active ingredients of Panax japonicus, a common medicinal plant growing in China, this study prepared the TSPJ, and investigated their pharmacological effects.…”