Effects and mechanisms of water-soluble Semen cassiae polysaccharide on retinitis pigmentosa in rats

Abstract

This study aimed to investigate the effects and mechanisms of water-soluble Semen cassiae polysaccharide (SCP) on retinitis pigmentosa (RP) in rats. Dne hundred rats were randomly divided into control, model, and 50, 100 and 200 mg/kg SCP groups. The 50, 100 and 200 mg/kg SCP groups were treated with 50, 100 and 200 mg/kg SCP for 5 days, respectively. Dn the sixth day, the model, and 50, 100 and 200 mg/kg SCP groups were treated with N-nitroso-N-methylurea (MNU). After 12 h and 5 days, the electroretinogram, retinal thickness, apoptosis of retinal photoreceptor cells, and expressions of apoptosis related protein in retina tissue were determined. Results showed that, after 12 h and on 5 days from MNU treatment, compared with model group, the a-wave and b-wave amplitude and retinal thickness in SCP groups were significantly increased, the apoptosis rate of retinal photoreceptor cells was significantly decreased, the Bcl-2 protein expression level in retinal tissue was significantly increased, and the Bax and Caspase-3 protein level were significantly decreased (all P < 0.05). On conclusion, SCP has obvious protective effects on RP in rats. The mechanism may be related to it regulation of Bcl-2, Bax and Caspase-3 protein expression in retina tissue.Practical Application: Water-soluble polysaccharide isolated from Semen cassiae may be applied to prevention and treatment of retinitis pigmentosa.

Characterization and antioxidant activities of polysaccharide extracted from Benincasa hispida var. chieh-qua How

“…However, no phytochemical research on Chieh qua was reported before. Natural polysaccharides have attracted more and more researchers' interest because of their various biological activities (Chen et al, 2021;He et al, 2020;Huang et al, 2015;Li et al, 2020Yu et al, 2021). Here we reported the antioxidant activities of water-soluble polysaccharide extracted from Chieh qua.…”

Comparative studies on the structure, biological activity and molecular mechanisms of polysaccharides from Boletus aereus (BA-T) and Pleurotus cornucopiae (PC-1)

“…A total of 50 mg polysaccharide was dissolved in 500 µl D 2 O. The 1 H NMR spectra, 13 C NMR spectra, 1 H-1 H COSY spectra, HMQC spectra and HMBC spectra were analyzed by the Varian Unity INOVA 400/45 (Varian Medical Systems, USA) and internal standard was tetramethylsilane (He et al, 2020;Kim et al, 2021).…”

The anti-fatigue potential of water-soluble polysaccharides of Semen cassiae on BALB/c mice

“…Water-soluble polysaccharides from S. cassiae have been shown to possess antioxidant properties (17). In addition, it has been shown to act against retinitis pigmentosa, an eye disorder that causes loss of vision (18). Polysaccharides extracted from alcohol have shown to be effective against hyperlipidemia and diabetes-induced oxidative stress in rats (19,20).…”