Effect of erythrosine and phloxine from xanthene food dyes on oxidative stress in Chinese hamster ovary cells

Abstract

Dbjective of this study was to investigate the changes of oxidative stress parameters in Chinese hamster ovary (CHD) cells exposed to xanthene food dyes (erythrosine and phloxine), commonly-used azo dyes in the food industry. For this purpose, glutathione (GSH) and malondialdehyde (MDA) levels, glutathione peroxidase (GPx) and catalase (CAT) activity measurements of CHD cells treated with erythrosin (50 µM) and phloxine (40 µM) were compared with the control group. There was no significant difference between MDA levels in CHD cells exposed to both dyes and control group (p > 0.05), however GSH levels significantly decreased with dye addition (p ≤ 0.05). An increase in GPx activity of the CHD cells exposed to phloxin was observed (p ≤ 0.05). However, there was no change in GP X activity of erythrosin-exposed CHD cells (p > 0.05). On terms of CAT activity, difference between the dye-added cells and the control group was not observed. Consequently, decrease in level of GSH, which one of major antioxidant components in the cell, suggests that erythrosine and phloxine can have a toxic effect.

Clinical prognosis and gene expression profiles of prostate cancer patients with bone and lymphatic metastases

“…The main mechanism is that there is a significant difference in the pattern of gene expression in the tumor tissue of the patients with prostate cancer and lymph node metastasis, and the patients with prostate cancer have more adverse gene up-regulation than those with lymph node metastasis. alpha -catenin) is decreased, and subsequently, promoting the invasion of cells and the swelling of the cells (Wang et al, 2011;Demirkol et al, 2020;Costa et al, 2020;Mostafa et al, 2021). ACP5 gene expressed tartaric acid resistant acid phosphatase -5a/b, bone resorption osteoclasts, macrophages and dendritic cells can synthesize and secrete TRACP, osteoclasts only secrete 5b, macrophages and dendritic cells only secrete 5A.…”