The present study reports the influence of two herbicides viz. Pendimethalin and imazethapyr on the changes in sugar, starch, protein, trypsin inhibitory activity (TIA), phenol content and antioxidant activity at the different stages of mung bean seed development. Each of the herbicides was applied at their recommended field dose (RFD) and double of the RFD (dRFD). Sugar content in seed was adversely affected by these herbicides, while starch and protein content were significantly increased at RFD of pendimethalin and imazethapyr respectively. Imazethapyr at dRFD registered lowest TIA level. Phenol content and antioxidant activity measured usingdiphenyl-2-picrylhydrazyl (DPPH), Azino-bis (3ethylbenzothiazoline-6-sulphonic acid (ABTS + ) and Ferric reducing antioxidant power (FRAP) assay, enhanced by pendimethalin treatment at RFD. Antioxidant activity under DPPH, ABTS and FRAP assays showed significant positive relation with phenol content (r= 0.99, 0.96 and 0.94 respectively) between them. DPPH assay produced higher absolute value for antioxidant activity as compared to other assay.