Anti-adipogenic effect of a turmeric extract-loaded nanoemulsion in 3T3-L1 preadipocytes and high fat diet-fed mice

Hwang, Jae Joon; Lee, Su Bi; Choi, Mi-Young; Kim, Jun Tae; Seo, Han Geuk

doi:10.1590/fst.20718

Cited by 9 publications

(12 citation statements)

References 28 publications

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“…In contrast to animal proteins, consumption of soy protein has been found in numerous studies to be adversely linked with BMI. Our findings, however, indicated that there was no evident change in BMI between mice fed milk protein or mice fed plant protein 5‐35 . Further, our findings showed that mice fed plant proteins had lower plasma insulin levels than mice fed milk proteins.…”

Section: Discussioncontrasting

confidence: 67%

“…The 3T3‐L1 pre‐adipocytes were seeded on a 96‐well plate at a density of 10 000 cells per well. The cytotoxicity activity of protein hydrolysates against 3T3‐L1 pre‐adipocytes was studied using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) reagent 17 . When cells reached 70–80% confluency, they were treated with varying concentrations of milk and plant protein hydrolysates for 48 h and then incubated in MTT solution for 4 h at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

“…To study the lipid‐lowering effects of protein hydrolysates, intracellular lipid accumulation was measured using ORO staining solution, as previously described 17 . 3T3‐L1 cells were seeded at a density of 3 × 10 4 cells per well in a 24‐well plate and maintained at 37 °C in a humidified air with 5% CO 2 until confluency reached 100%.…”

Section: Methodsmentioning

confidence: 99%

“…The cytotoxicity activity of protein hydrolysates against 3T3-L1 pre-adipocytes was studied using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent. 17 When cells reached 70-80% confluency, they were treated with varying concentrations of milk and plant protein hydrolysates for 48 h and then incubated in MTT solution for 4 h at 37 °C. When MTT incubation was completed, dimethyl sulfoxide (DMSO) was added to each well and mixed thoroughly to dissolve the formazan crystals.…”

Section: Cell Cytotoxicity Assaymentioning

confidence: 99%

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Comparative evaluation of milk proteins and oil‐seed‐cake‐derived proteins extracted by chemical and biological methods for obesity management

Sharma,

Lande,

Sameni

et al. 2023

J Sci Food Agric

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BackgroundIn light of the exponential rise in global population, there is a critical requirement to reduce food waste on a global scale. According to the studies, agricultural wastes like oil‐seed cakes offer great nutritional value. Acid precipitation (A) and alkaline extraction methods (traditional methods) were used to extract protein from oil‐seed cakes, however both procedures are linked to decreased protein quality and quantity, which prompted the development of a novel strategy known as the biological/microbial/probiotic (B) method. Therefore, the present study aimed to highlight the optimal way of protein extraction from oil‐seed cakes and the effect of extraction methods on protein efficacy against obesity. The outcomes were also compared with milk proteins.ResultsIn‐vitro study provided evidences that proteins from both the sources (plant and milk) suppressed adipogenesis and stimulated adipolysis in 3T3L‐1 cells. For in‐vivo study, mice were fed with different protein extracts soya protein (SPP), ground protein (GPP), whey protein (WP) and casein protein (CP)) containing 40% of their calories as fat. Body weight decreased significantly in all the rats except CP fed rats. BMI, atherogenic index, plasma TG and VLDL‐C level decreased significantly in all the groups in comparison to model group (HFD group), but the decrease was more pronounced in plant proteins than milk proteins. In hepatocytes, the expression of FIAF, CPT1 and PPAR‐α genes was increased significantly in SPP fed groups. Adiponectin gene expression was up regulated significantly in visceral fat tissue in groups fed SPP‐B, GPP‐A and CP, whereas leptin gene was down regulated significantly in all the groups except SPP‐A.ConclusionsThis study demonstrates that SPP‐B has shown the most effective anti‐obesity property followed by WP. Additionally, we found that biological precipitation approach produced better outcomes for plant proteins isolated from oil‐seed cakes than the acid precipitation method.This article is protected by copyright. All rights reserved.

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Section: Discussioncontrasting

confidence: 67%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Cell Cytotoxicity Assaymentioning

confidence: 99%

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Comparative evaluation of milk proteins and oil‐seed‐cake‐derived proteins extracted by chemical and biological methods for obesity management

Sharma,

Lande,

Sameni

et al. 2023

J Sci Food Agric

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“…The absorbance of the sample was determined at 570 nm. 7 Three independent experiments were performed, each in triplicate.…”

Section: Cell Viability Assaymentioning

confidence: 99%

Effect of Aporosa villosa Stem Ethanolic Extract on Adipogenesis in 3T3-L1 Adipocytes

Nanna¹,

Chularojmontri²,

Tingpej³

et al. 2021

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Lipid‐lowering activity and underlying mechanism of glycosylated peptide–calcium chelate prepared by transglutaminase pathway

Wu,

Chen,

Cai

et al. 2023

Food Frontiers

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The compounds that promote calcium absorption and osteogenesis may have great potential in controlling adipogenesis and obesity. In this study, the glycosylated peptides–calcium chelate prepared from Crimson Snapper scale protein hydrolysates (CSPHs) and xylooligosaccharide (XOS) via transglutaminase pathway with calcium absorption promoting and osteogenic activities, named XOS‐CSPHs‐Ca‐TG, were used to explore the potential prebiotic effect and lipid‐lowering activity on 3T3‐L1 cells. The presence of XOS‐CSPHs‐Ca‐TG in pure cultures could significantly promote the proliferation of probiotics (Bifidobacterium adolescentis and Lactobacillus acidophilus), enabling them to enter the logarithmic growth phase 2–4 h earlier. Results of in vitro fermentation of mice intestinal flora further indicated that XOS‐CSPHs‐Ca‐TG increased the number of beneficial bacteria such as Bifidobacterium and Lactobacillus by 11.57% and 7.63%, respectively, and boosted the production of short‐chain fatty acids, thereby improving the colonization ability of the intestinal microbiota. Results of quantitative real‐time polymerase chain reaction confirmed that XOS‐CSPHs‐Ca‐TG achieved lipid‐lowering effects by accelerating calcium influx and activating the mitogen‐activated protein kinase/extracellular signal‐regulated kinases signaling pathway, thereby inhibiting the gene expressions of the downstream peroxisome proliferator‐activated receptors γ and CCAAT/enhancer‐binding protein α, and further attenuating the secretion of adipokines and controlling the expression of key lipases. These results suggest that glycosylated peptides–calcium chelates are potentially functional foods in lipid metabolism‐related disease prevention.

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Anti-adipogenic effect of a turmeric extract-loaded nanoemulsion in 3T3-L1 preadipocytes and high fat diet-fed mice

Cited by 9 publications

References 28 publications

Comparative evaluation of milk proteins and oil‐seed‐cake‐derived proteins extracted by chemical and biological methods for obesity management

Comparative evaluation of milk proteins and oil‐seed‐cake‐derived proteins extracted by chemical and biological methods for obesity management

Effect of Aporosa villosa Stem Ethanolic Extract on Adipogenesis in 3T3-L1 Adipocytes

Lipid‐lowering activity and underlying mechanism of glycosylated peptide–calcium chelate prepared by transglutaminase pathway

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