Effect of Aporosa villosa Stem Ethanolic Extract on Adipogenesis in 3T3-L1 Adipocytes

Nanna, Urarat; Chularojmontri, Linda; Tingpej, Pholawat; Kaewamatawong, Rawiwun; Homhual, Sudarat; Suwannaloet, Wanwisa; Fuangfoo, Thanes; Naowaboot, Jarinyaporn

doi:10.5530/pj.2021.13.180

Cited by 1 publication

(1 citation statement)

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“…Since B. solanifolium , presents active reported NPs, both extracts were no further considered. For A. villosa , some reports already described various bioactivities of ethanolic extracts from different plant parts were reported ( Srikrishna et al, 2008 ; Venkataraman et al, 2010 ; Nanna et al, 2021 ). Additionally, preliminary metabolite profiling indicated a high concentration of fatty acids.…”

Section: Resultsmentioning

confidence: 99%

Integration of Wnt-inhibitory activity and structural novelty scoring results to uncover novel bioactive natural products: new Bicyclo[3.3.1]non-3-ene-2,9-diones from the leaves of Hymenocardia punctata

Quiros-Guerrero,

Marcourt,

Chaiwangrach

et al. 2024

Front. Chem.

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In natural products (NPs) research, methods for the efficient prioritization of natural extracts (NEs) are key for discovering novel bioactive NPs. In this study a biodiverse collection of 1,600 NEs, previously analyzed by UHPLC-HRMS2 metabolite profiling was screened for Wnt pathway regulation. The results of the biological screening drove the selection of a subset of 30 non-toxic NEs with an inhibitory IC50 ≤ 5 μg/mL. To increase the chance of finding structurally novel bioactive NPs, Inventa, a computational tool for automated scoring of NEs based on structural novelty was used to mine the HRMS2 analysis and dereplication results. After this, four out of the 30 bioactive NEs were shortlisted by this approach. The most promising sample was the ethyl acetate extract of the leaves of Hymenocardia punctata (Phyllanthaceae). Further phytochemical investigations of this species resulted in the isolation of three known prenylated flavones (3, 5, 7) and ten novel bicyclo[3.3.1]non-3-ene-2,9-diones (1, 2, 4, 6, 8–13), named Hymenotamayonins. Assessment of the Wnt inhibitory activity of these compounds revealed that two prenylated flavones and three novel bicyclic compounds showed interesting activity without apparent cytotoxicity. This study highlights the potential of combining Inventa’s structural novelty scores with biological screening results to effectively discover novel bioactive NPs in large NE collections.

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Section: Resultsmentioning

confidence: 99%