In vitro potential activity of some seaweeds as antioxidants and inhibitors of diabetic enzymes

Abstract

In this study crude extracts of Turbinaria decurrens, Padina pavonica, Sargassum muticum and Sargassum acinarium (Phaeophyta); Ulva lactuca (Chlorophyta) and Pterocladia capillacea (Rhodophyta) seaweeds were tested to evaluate their antioxidant properties and antidiabetic potential on α-amylase and α-glucosidase starch hydrolyzing enzymes. The results showed that all analyzed seaweeds exhibited antioxidant effects using DPPH (2,2-Diphenyl-1-picrylhydrazyl), reducing power and total antioxidant capacity assays in addition to antidiabetic activity that all depended on the species and the extract solvent. Among the tested extracts, acetone extract of Turbinaria decurrens showed the highest antioxidant activity and inhibitory effects for α-amylase (96.1%) and α-glucosidase (97.4%), respectively which was related with its total phenolic content and antioxidant activity. In vitro, the extract showed no toxicity against fibroblast normal cell lines at lower concentration of 250 µg/ml. Gas Chromatography-Mass Spectrum analysis (GC-MS) of this acetone extract showed the presence of different bioactive compounds mainly cyclotrisiloxane, hexamethyl which could be responsible for its antioxidant and antidiabetic activities. The study results suggest that brown seaweeds especially T. decurrens can be used as antioxidant ingredients and as potent reducing drug for postprandial hyperglycemia.

Antioxidant and Antihyperglycemic Activity of Arthrospira platensis (Spirulina platensis) Methanolic Extract: In vitro and In vivo Study

“…α-amylase and absorb glucose by intestinal α-glucosidase, which may swift postprandial hyperglycemia (Ismail et al, 2019). At this time, the offered medication for treating type 2 diabetes performances through the inhibition of these diabetic related enzymes (Rang et al, 2003).…”

Comparative studies on the structure, biological activity and molecular mechanisms of polysaccharides from Boletus aereus (BA-T) and Pleurotus cornucopiae (PC-1)

“…Silane reagent was was used to prepare derivatized product detected by GC-MS (Agilent 7890A, USA). The initial temperature set at 80 °C and maintained for 3 min, with a linear increase to 200 °C at a rate of 10 °C/min, then maintaining at 200 °C for 10 min (Ismail et al, 2020;Sun et al, 2020;Yang et al, 2020).…”

Bioactive Secondary Metabolites of Wild Antheraea mylitta Silkworm Cocoons

“…As well as in the diet, it is used in hardening soaps, softening plastics and in making cosmetics, candles and plastics. It is a stearic acid ester reported for its antioxidant and anti-inflammatory activity [69]. The alcoholic compound of oxalic acid is reported for antimicrobial preservative [70].…”