Optimization of ultrasound-homogenization combined extraction of phenolics in peony roots and leaves

Abstract

Paeonia ostii is the representative of oil-utilized peony, its roots (PR) and leaves (PL) are discarded as by-products, resulting in a waste of resources. The exploration of extraction process of active ingredients from PR/PL is beneficial to the comprehensive utilization. In this work, the optimum process of Ultrasound-Assisted Extraction (UAEo) of total flavonoids content (TFC) from PR and PL was determined by single factor and response surface methodology. The results showed that UAEo was 80 min, 1:5 g/mL, 250 W, 33.83 mg Rutin/g dw for PR and 60 min, 1:10 g/mL, 250 W, 25.85 mg Rutin/g dw for PL. Then, Homogenization-Assisted Extraction (HAE), Homogenization-Ultrasound-Assisted Extraction (HUAEo) and Ultrasound-Homogenization-Assisted Extraction (UHAEo) were further analyzed. The highest PR(PL)-TFC by HUAEo at homogenization 5 min was 49.58 ± 0.25 mg/g with an increase of 46.6% (33.02 ± 0.04 mg/g with an increase of 27.7%). The highest PR/PL-total phenolic content by the HUAEo reached 77.84 ± 0.52 mg/g dw and 146.62 ± 2.77 mg/g dw for homogenization 3 min. However, there was no significant difference between HUAEo and UHAEo. In conclusion, the TFC increased with the extension of HAE time, and the combined extraction was higher yield than the single extraction.

An Integrated Extraction–Purification Process for Raspberry Leaf Polyphenols and Their In Vitro Activities

“…The R 2 value of the model was 0.9920, which meant that 99.20% of the variation in response values came from the selected variable, meaning that the model was consistent with the experimental results. This indicator is similar to the results of other RSE studies [16]. In addition, the misfitting term was not significant (p = 0.3815 > 0.05), while the Adj R 2 and the Pred R 2 were 0.9818 and 0.9299, and their difference was less than 0.2.…”

“…The TPC of the extract obtained using HG + US reached about 300 mg GAE/g, which is approximately double that of the extract obtained using a single process [15]. Wang et al [16] also extracted phenolic compounds from the roots and leaves of peony using a combined process. The TPCs from the roots and leaves reached about 80 mg GAE/g and 150 mg GAE/g, respectively, values which were about 50% higher than those obtained using a single process.…”

Effect of ultrasonic treatment on the quality of Mianning ham

“…A rise in ultrasound temperature did not entirely trigger the enzymatic activity in the ham at lower temperatures, ultimately leading to an increase in ham hardness. At higher temperatures, the loss of water in the muscle protein of ham resulted in less hardness ( 36 ). The increase in ultrasonic power and the amplified cavitation effect of ham caused a loss of internal moisture, leading to a reduction in ham hardness.…”

Insights into sub-chronic toxicity effects of enzymatic hydrolysate of peony seed meal derived Maillard reaction products in SD rats

“…Peony is widely planted in China, and peony seeds contain high omega-3 fatty acids (Qu et al, 2017;Wang et al, 2022). The polysaccharides extracted from peony seed dreg displayed high anti-oxidant (Shi et al, 2016a;Shi et al, 2016b;Zhang et al, 2022), and anti-cancerous properties (Zhang et al, 2017).…”