Use of the polymerase chain reaction for the diagnosis of American tegumentary leishmaniasis

Intralesional administration of meglumine antimoniate (MA) as an alternative treatment for cutaneous leishmaniasis: a case report

“…The second sample was used for imprint with staining, which also revealed no amastigote forms of the parasite. Subsequently, the same material was preserved in sterile saline solution, macerated with 300μg of extraction buffer, and frozen in a properly identified flask for PCR as described by Barçante et al, 2019. After analyzing the electrophoretic pattern of the sample with the positive control, the presence of Leishmania spp.…”

Sensitive and specific serodiagnosis of tegumentary leishmaniasis using a new chimeric protein based on specific B-cell epitopes of Leishmania antigenic proteins

“…Parasitological tests, which are based on the direct examination of amastigotes in Giemsa-stained lesion smears of scrapping, biopsies or impression smears, have been used with such purpose. However, due to the scarcity of parasites in the collected samples, mainly in patients with old lesions, the sensitivity is variable and between 20% and 40% of the patients present false-negative results [6][7][8][9]. Molecular tests have also been used as more sensitive tools to detect Leishmania content in collected samples.…”