Sensitive and specific serodiagnosis of tegumentary leishmaniasis using a new chimeric protein based on specific B-cell epitopes of Leishmania antigenic proteins

Galvani, Nathália Coral; Machado, Amanda S.; Lage, Daniela P.; Martins, Vívian T.; Oliveira, Danielle Meireles de; Freitas, Camila S.; Vale, Danniele L.; Fernandes, Bruna Barros; Oliveira‐da‐Silva, João A.; Reis, Thiago A.R.; Santos, Thaís T.O.; Ramos, Fernanda F.; Bandeira, Raquel S.; Ludolf, Fernanda; Tavares, Grasiele S.V.; Guimarães, Nathália Sernizon; Tupinambás, Unaí; Chávez-Fumagalli, Miguel Á.; Humbert, María Victoria; Gonçalves, Denise Utsch; Christodoulides, Myron; Machado-de-Ávila, Ricardo Andrez; Coelho, Eduardo Antônio Ferraz

doi:10.1016/j.micpath.2021.105341

Cited by 5 publications

(6 citation statements)

References 56 publications

(72 reference statements)

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“…[ 82 ], in which the cases were selected from a health service or hospital. To assess the performance of peptide-based tests, three different formats were reported: one study used an ICT format [ 7 ], two studies used phage-ELISA [ 15 , 62 ] and 19 studies used an ELISA technique [ 10 , 11 , 16 , 17 , 26 , 27 , 29 , 35 , 37 , 40 – 43 , 47 , 48 , 58 , 63 , 69 , 70 ]. In these studies using an ELISA method, a marked difference was observed in the amount of peptides used, which ranged from 0.25 μg to 20 μg per well.…”

Section: Resultsmentioning

confidence: 99%

“…Two publications assessed only CL diagnosis with cases from Brazil (one study) [ 35 ] and from Peru (one study) [ 10 ]. Finally, six studies assessed peptides diagnosis with TL and VL cases from Brazil in a separate group [ 26 , 27 , 41 – 43 ] or in the same group [ 70 ]. Most of the TL cases studied were due to L. braziliensis infection and the VL cases due to L. infantum .…”

Section: Resultsmentioning

confidence: 99%

“…Different reference standards were used to confirm leishmaniasis cases ( Table 1 ). Thirteen studies employed a composite reference test using two methods (parasitological and molecular techniques n = 7 [ 15 , 17 , 27 , 40 – 43 ], or parasitological and serological techniques n = 1 [ 7 ] or serological and molecular techniques n = 5 [ 16 , 26 , 37 , 48 , 58 ]). Two studies employed a composite reference test using three methods (parasitological, molecular and serological techniques) [ 62 , 63 ].…”

Section: Resultsmentioning

confidence: 99%

“…Fifteen articles used a bioinformatic method. Five different algorithms were cited: ABCPred [ 7 , 26 , 27 , 37 , 62 , 63 , 69 ], BepiPred [ 7 , 11 , 17 , 40 – 43 ], IEDB [ 26 , 27 , 47 , 70 ], EpiQuest-B and LBTope [ 7 ]. Other strategies for epitope discovery were phage-displayed immunoscreening of random peptides libraries [ 15 , 16 , 35 , 48 , 58 ] or construction of an overlapping peptide library from the candidate protein sequence [ 10 , 29 ].…”

Section: Resultsmentioning

confidence: 99%

“…Most studies included in this review estimated the performance of peptide-based tests with sera of patients from single geographical areas (Sudan, Brazil or Peru) and infected by a same Leishmania species (molecularly identified or assumed), either by L. infantum for VL diagnosis or L. braziliensis for TL diagnosis. Only six studies, all performed in Brazil, evaluated the performance of the test using both sera of L. infantum VL patients and L. braziliensis TL patients [ 26 , 27 , 41 – 43 , 70 ].…”

Section: Discussionmentioning

confidence: 99%

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A systematic review of peptide-based serological tests for the diagnosis of leishmaniasis

et al. 2023

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Serological methods should meet the needs of leishmaniasis diagnosis due to their high sensitivity and specificity, economical and adaptable rapid diagnostic test format, and ease of use. Currently, the performances of serological diagnostic tests, despite improvements with recombinant proteins, vary greatly depending on the clinical form of leishmaniasis and the endemic area. Peptide-based serological tests are promising as they could compensate for antigenic variability and improve performance, independently of Leishmania species and subspecies circulating in the endemic areas. The objective of this systematic review was to inventory all studies published from 2002 to 2022 that evaluate synthetic peptides for serological diagnosis of human leishmaniases and also to highlight the performance (e.g., sensitivity and specificity) of each peptide reported in these studies. All clinical forms of leishmaniasis, visceral and tegumentary, and all Leishmania species responsible for these diseases were considered. Following PRISMA statement recommendations, 1,405 studies were identified but only 22 articles met the selection criteria and were included in this systematic review. These original research articles described 77 different peptides, of which several have promising performance for visceral or tegumentary leishmaniasis diagnosis. This review highlights the importance of and growing interest in synthetic peptides used for serological diagnosis of leishmaniases, and their performances compared to some widely used tests with recombinant proteins.

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