Recurrent aphthous stomatitis in 18-year-old adolescents - Prevalence and associated factors: a population-based study

Souza, Paulo Ricardo Martins; Duquia, Rodrigo Pereira; Breunig, Juliano de Avelar; Almeida, Hiram Larangeira de

doi:10.1590/abd1806-4841.20174692

Cited by 16 publications

(11 citation statements)

References 10 publications

(12 reference statements)

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“…Furthermore, the prevalence of RAS was 55% and 35.6% discovered in the dental clinic as of 2010–2012 and 2014-2015, respectively [ 1 , 2 ]. Other studies carried out in the midst of the population of 18-year-old adolescents in Brazil discovered to be 24.9% [ 3 ], about 10.84% RAS were also discovered in Turkey [ 4 ], 14% was reported in the female dental students in King Khalid University Saudi Arabia [ 5 ], and 29.38% was reported in the college students in Beijing University of Chinese Medicine [ 6 ]. RAS is considered as a multifactorial process of unknown etiology, in which various triggering factors and an immunological disturbance are combined, including genetic [ 7 , 8 ], vitamin B-12, folate, and iron deficiency [ 9 , 10 ] and an allergic condition, such as atopy [ 11 , 12 ] Pain or unpleasant feeling and its recurrency in the oral cavity are always complained by an individual that has RAS, and this condition makes researchers interested in discovering the pathogenesis of RAS and its ultimate goal in finding the appropriate novel therapy [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

The Impact of DNA Methylation on IL6 mRNA Levels in Hematinic Deficiency and Atopy-Associated Recurrent Aphthous Stomatitis Patients

Nur’aeny

Gurnida

Suwarsa

et al. 2021

International Journal of Dentistry

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Objective. To investigate the DNA methylation using pyrosequencing and its effects on the upregulation of IL6 mRNA in patients with recurrent aphthous stomatitis (RAS) in connection with hematinic deficiency and atopy. Material and Methods. This cross-sectional study was conducted at Dr. Hasan Sadikin Hospital, Bandung, from January–March 2019 and was approved by the Health Research Ethics Committee of Universitas Padjadjaran (Ethics No. 990/UN6.KEP/EC/2018). Furthermore, the subjects had RAS ulcers with a history of at least twice a year along with atopy and dietary imbalance with no history of recurrent intraoral herpes or any systemic diseases. This study was performed on 23 RAS patients and 21 healthy subjects, and the sampling was carried out consecutively. The blood samples were collected from all the subjects, and then, the DNA and RNA were extracted from the peripheral blood mononuclear cells (PBMCs). Consequently, the bisulfite-modified DNA was used to confirm the methylation status of the IL6 gene promoter through the pyrosequencing method. The methylation levels of the IL6 promoter were assessed by a reverse transcriptase-polymerase chain reaction technique. The gene expression of RAS and the control group was analyzed by the 2−ΔΔCT method. The statistical analysis using the Mann–Whitney U test was conducted to evaluate IL6 mRNA levels and DNA methylation with p value <0.05 considered to be statistically significant. Result. The IL6 mRNA levels were approximately 1.88-fold in RAS patients, and there was a significant relationship between the expression of the IL6 gene and the increased risk of RAS p < 0.001 . It was reported that four out of six sites in the cytosine phosphate guanine (CpG) island IL6 promoter had a lower degree of methylation, and two other sites in patients with RAS had greater methylation compared with control, but not statistically significant. Conclusion. This study showed the upregulation of IL6 mRNA levels in RAS patients compared to control. DNA methylation in the present study is at sites 566–658, whereas the location of the IL6 promoter is at sites 1–1684. Thus, it would be necessary conducting some research at other CpG sites of IL6 promoter islands to determine the status of DNA methylation.

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Section: Introductionmentioning

confidence: 99%

The Impact of DNA Methylation on IL6 mRNA Levels in Hematinic Deficiency and Atopy-Associated Recurrent Aphthous Stomatitis Patients

Nur’aeny

Gurnida

Suwarsa

et al. 2021

International Journal of Dentistry

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“…In this cross-sectional study, we explored the distribution of RAS, dietary factors, self-reported trigger factors, and therapeutic methods among a study population from Nanjing and reported a prevalence of 21.4% in accordance with the literature [14,24,25]. Moreover, less than half (72, 44.7%) of them had ever received treatment for RAS, which was lower than the percentage reported in another study [21,26], and nearly one-third participants who have ever treated RAS mainly use ATs instead of conventional medicine, despite a lack of randomized controlled trials to prove the effectiveness and safety of these ATs.…”

Section: Discussionmentioning

confidence: 72%

Relationship between dietary factors and recurrent aphthous stomatitis: A cross-sectional study

Zhou

Fan

et al. 2020

Preprint

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Background: Recurrent aphthous stomatitis (RAS), a common oral mucosal disorder characterized by chronic, inflammatory, and ovoid ulcers, has a complex aetiology. The purpose of the study was to investigate the specific dietary factors influencing the prevalence of RAS.Methods: A total of 754 participants aged 18 to 59 years in Nanjing were enrolled in this descriptive cross-sectional study. An anonymous questionnaire was adopted to investigate the distribution of RAS, dietary factors, self-reported trigger factors, and therapeutic methods.Results: Among all participants, the prevalence rate of RAS was 21.4%. The univariable analysis suggested fruits, dairy products, vegetables, and drinking water, but not fried foods, fermented foods, spicy foods, and eggs, as preventive factors of RAS. After adjusting for age and sex, multivariable regression analysis suggested fruits (adjusted odds ratio [aOR] = .430, 95% confidence interval [CI] = [.218-.847], p = .015) and drinking water (aOR = .294, 95% CI = [.119-.726], p = .008) as protective factors of RAS. Conclusion: This study suggests that consumption of fruit and drinking water is negatively associated with RAS and dietary factors play an important role in RAS development. These results imply a potential adjunctive and complementary role of food in RAS treatment and some potential, simple, and feasible means for RAS prevention.

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“…In Iran, the prevalence of RAS is 25.2% (10,291 respondents), in Brazil, 24.9% (2,427 respondents), and in Turkey, 22.8% (11,360 respondents). [12][13][14] In this study, RAS was associated with fruits, vegetables, dairy products and drinking water intake through univariable analysis (p < .05). Other variables, including fried foods, fermented foods, spicy foods, eggs, gender and age, showed no statistically signi cant associations (p ≥ .05).…”

Section: Associations Of Dietary Factors With Rasmentioning

confidence: 65%

Relationship between dietary factors and recurrent aphthous stomatitis: A cross-sectional study

Zhou

Fan

et al. 2020

Preprint

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Background: Recurrent aphthous stomatitis (RAS), a common oral mucosal disorder characterized by chronic, inflammatory, and ovoid ulcers, has a complex aetiology. The purpose of the study was to investigate the dietary factors influencing the prevalence of RAS.Methods: A total of 754 participants aged 18 to 59 years in Nanjing were enrolled in this descriptive cross-sectional study. An anonymous questionnaire was adopted to investigate the distribution of RAS, dietary factors, self-reported trigger factors and therapeutic methods.Results: Among all participants, the prevalence rate of RAS was 21.4%. Univariable analysis identified fruits, dairy products, vegetables and drinking water, but not fried foods, fermented foods, spicy foods, or eggs, as influencing factors of RAS. After adjusting for age and sex, multivariable regression analysis identified fruits (adjusted odds ratio [aOR] = .432, 95% confidence interval [CI] = [.219-.853], p = .015) and drinking water (aOR = 3.603, 95% CI = [1.280-10.147], p = .015) as protective factors of RAS. Conclusion: RAS is prevalent among the 18- to 59-year-old Nanjing population. A lower intake of fruits and drinking water might be associated with a higher prevalence of RAS. These factors could be used as daily preventive measures for RAS.

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Recurrent aphthous stomatitis in 18-year-old adolescents - Prevalence and associated factors: a population-based study

Cited by 16 publications

References 10 publications

The Impact of DNA Methylation on IL6 mRNA Levels in Hematinic Deficiency and Atopy-Associated Recurrent Aphthous Stomatitis Patients

The Impact of DNA Methylation on IL6 mRNA Levels in Hematinic Deficiency and Atopy-Associated Recurrent Aphthous Stomatitis Patients

Relationship between dietary factors and recurrent aphthous stomatitis: A cross-sectional study

Relationship between dietary factors and recurrent aphthous stomatitis: A cross-sectional study

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