2015
DOI: 10.1590/abd1806-4841.20153331
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Abstract: Type I collagen is the main dermal component, and its evaluation is relevant to quantitative studies in dermatopathology. However, visual gradation (0 to 4+) has low precision and high subjectivity levels. This study aimed to develop and validate a digital morphometric analysis technique to estimate type I collagen levels in the papillary dermis. Four evaluators visually quantified (0 to 4+) the density of type I collagen in 63 images of forearm skin biopsies marked by immunohistochemistry and two evaluators a… Show more

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Cited by 17 publications
(13 citation statements)
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“…Type I collagen is the main component of the dermis and is also found in tendons, bones, and cartilage. As the predominant component of the extracellular matrix, type I collagen plays an important role in maintaining and connecting ability and tenacity of this supporting structure (Moharamzadeh et al , ; Brianezi et al , ). Type I collagen is frequently used to precoat culture plates to improve cell culture conditions.…”
Section: Discussionmentioning
confidence: 99%
“…Type I collagen is the main component of the dermis and is also found in tendons, bones, and cartilage. As the predominant component of the extracellular matrix, type I collagen plays an important role in maintaining and connecting ability and tenacity of this supporting structure (Moharamzadeh et al , ; Brianezi et al , ). Type I collagen is frequently used to precoat culture plates to improve cell culture conditions.…”
Section: Discussionmentioning
confidence: 99%
“…The photos represented interfollicular spaces and were captured in triplicate for each slide under 40x magnification. The thicknesses of the stratum corneum, epithelium (measured from the basal layer to the upper granular layer), and granular layer (measured as the density of melanin and collagen in the upper dermis) was measured digitally using Image J software 1.46 [7,[17][18][19].…”
Section: Histopathology and Immunohistochemistrymentioning
confidence: 99%
“…The scientific literature describes the colour segmentation technique used in the present study in detail, including in several contemporary studies that have assessed the applicability of K-means clustering in dermatological research [5,4,12]. This study opted for the target colour, as binarisation methods commonly employed in morphology can overestimate the values resulting from the absence of sample categorisation in different groups [18].…”
Section: Discussionmentioning
confidence: 99%