A simple and sensitive method for determination of Norfloxacin in pharmaceutical preparations

Abstract

In this approach, a new voltammetric method for determination of norfloxacin was proposed with high sensitivity and wider detection linear range. The used voltammetric sensor was fabricated simply by coating a layer of graphene oxide (GO) and Nafion composited film on glassy carbon electrode. The advantage of proposed method was sensitive electrochemical response for norfloxacin, which was attributed to the excellent electrical conductivity of GO and the accumulating function of Nafion under optimum experimental conditions, the present method revealed a good linear response for determination of norfloxacin in the range of 1×10-8 mol/L-7×10 -6 mol/L with a detection limit of 5×10 -9 mol/L. The proposed method was successfully applied in the determination of norfloxacin in capsules with satisfactory results.Uniterms: Norfloxacin/determination. Graphene oxide. Nafion. Voltammetry/differential pulse. Pharmaceutical formulations/quantitative analysis.Propos-se, por essa abordagem, novo método voltamétrico, com alta sensibilidade e faixa linear de detecção mais ampla, para a determinação de norfloxacino. O sensor voltamétrico utilizado foi fabricado simplismente por cobertura de camada de óxido de grafeno (GO) e filme de Nafion em eletrodo de cabrono vítreo. A vantagem do método proposto foi a resposta eletroquímica sensível para o norfloxacino, atribuída à condutividade elétrica excelente do GO e à função acumulada do Nafion. Sob condições experimentais ótimas, o presente método revelou boa resposta linear para a determinação do norfloxacino na faixa de limite de detecção de 1×10 -8 mol/L-7×10 -6 mol/L. O método proposto foi aplicado com sucesso na determinação de norfloxacino em cápsulas, com resultados satisfatórios.Uniterms: Norfloxacino/determinação. Óxido de grafeno. Nafion. Voltametria de pulso diferencial. Formulações farmacêuticas/análise quantitativa.

High-sensitive electrochemical sensor for determination of Norfloxacin and its metabolism using MWCNT-CPE/pRGO-ANSA/Au

“…Comparison of the fabricated sensor with reported sensors for NFX determination. the performance of MWCNT-CPE/pRGO-ANSA/Au with previously reported electrochemical sensors [2,3,13,[36][37][38]. This proposed MWCNT-CPE/pRGO-ANSA/Au sensor shows either lower detection limit [2,3,13,37,38] or relatively broad linear rang [2,3,13,[36][37][38] for NFX determination, except for device simplicity.…”

“…the performance of MWCNT-CPE/pRGO-ANSA/Au with previously reported electrochemical sensors [2,3,13,[36][37][38]. This proposed MWCNT-CPE/pRGO-ANSA/Au sensor shows either lower detection limit [2,3,13,37,38] or relatively broad linear rang [2,3,13,[36][37][38] for NFX determination, except for device simplicity. In addition, the proposed sensor was successfully applied in both pharmaceutical formulations and plasma samples.…”

Dispersion of multi-walled carbon nanotubes in [BMIM]PF 6 for electrochemical sensing of acetaminophen

“…The reason could be attributed to the negative charge of Nafion®, which blocked the diffusion of ferrocyanide and ferricyanide towards the electrode surface, and thus completely hindering the electron and mass transfer. This was due to the electrostatic (negative charge) repulsive effects between Nafion® and [Fe(CN)6]3−/4− [[37],[38],[39]], with no well-defined peak in cyclic voltammetry experiments for Nafion®/GCE there was defined peak as shown inFig. 2.…”