Simple and rapid spectrophotometric assay of albendazole in pharmaceuticals using iodine and picric acid as CT complexing agents

Abstract

Two simple, rapid and inexpensive spectrophotometric methods are described for the determination of albendazole (ALB) in bulk drug and in tablets. The methods are based on charge-transfer (CT) complexation reaction involving ALB as n-donor and iodine as σ-acceptor (method A) in dichloromethane or picric acid (PA) as π-acceptor (method B) in chloroform. The absorbance of CT complexes was measured at 380 nm for method A, and 415 nm for method B. The optimization of the experimental conditions is described. Under optimum conditions, Beer's law obeyed over the concentration ranges 8.0-240 and 2.4-42 μg mL-1 for method A and method B, respectively. The apparent molar absorptivity of CT complexes at the respective λmax are calculated to be 1.17×103 and 5.22×103 L mol-1cm-1 respectively, and the corresponding Sandell sensitivity values are 0.2273 and 0.0509 ng cm-2. The limits of detection (LOD) and quantification (LOQ) are calculated to be (0.69 and 2.08), and (0.10 and 0.30) μg mL-1 with method A, and method B, respectively. The intra-day and inter-day accuracy expressed as % RE and precision expressed as % RSD were less than 3%. The methods were applied to the determination of ALB in tablets.

Facile complexation reactions for the selective spectrofluorimetric determination of albendazole in oral dosage forms and spiked human plasma

“… 2 Due to the great clinical importance of ABZ, several analytical methods have been reported for its determination either in pharmaceutical dosage forms or in biological fluids. The reported analytical methods include: spectrophotometry, 3–9 spectrofluorimetry, 10–13 high-performance liquid chromatography (HPLC) 14–17 HPTLC, 18 thermogravimetry 19 and electrochemical methods. 20,21 …”

Spectrophotometric/Titrimetric Drug Analysis

“…The charge-transfer complex is formed from a combination of two molecules, one of which acts as an electron donor and the other as an electron acceptor. Based on the charge-transfer complexation, albendazole with picric acid [58] (Figure 4), chloranilic with tyrosine [59], carvedilol with iodine [60] to name a few, were determinates using spectrophotometric techniques.…”

Quantification of albendazole in tablets of combined composition

“…In the literature for the quantitative determination of albendazole in mono-component tablets is conducted by the method of absorption spectrophotometry in the ultraviolet spectrum in an environment of methanol acidified with hydrochloric acid [7], in the visible region after reaction with 2,3-dichloro-5,6-dicyano-pbenzoquinone or 3,6-dichloro-2,5-dihydroxypbenzoquinone [8] and complexation reactions with iodine or picric acid [9].…”