Assessment of mutagenic and antimutagenic effects of Punica granatum in mice

Valadares, Marize Campos; Pereira, Enir Raquel Tavares; Benfica, Polyana Lopes; Paula, José Realino de

doi:10.1590/s1984-82502010000100014

Cited by 23 publications

(11 citation statements)

References 43 publications

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“…TBARS results are dose-dependent (Horn et al 2016). TBARS was significantly increased in mice liver exposed to 25 mg kg -1 of CP for 10 days (Zarei and Shivanandappa 2013), also in mice 24 hours after having been administrated one dose of 200 mg kg -1 CP (Valadares et al 2010), and in the kidney of rats exposed to 27 mg kg -1 CP administered four times in ten weeks (Kocahan et al 2017). In a test of rutin as a potential inhibitor of the oxidative stress caused by CP, the levels of lipid peroxidation also remained unchanged (Nafees et al 2015).…”

Section: Resultsmentioning

confidence: 99%

First phytochemical and biological study of the ethanolic extract from leaves of Capirona decorticans (Rubiaceae)

et al. 2018

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Capirona decorticans (Rubiaceae) is popularly used to treat warts, wounds, mycoses and scabies, and is also a component of the Ayahuasca tea. Despite its popular use, the phytochemical and pharmacological research on this species is limited. Therefore, this work quantified phenolic compounds in the ethanolic extract (EE) and hydromethanolic fraction (FM) (406, 293 mgEAG g-1, respectively) from leaves of C. decorticans. We identified flavonoids by LC-MS/MS-MMR-ESI (apigenin, rutin, luteolin, miricetin, quercetin, quercetin-3-β-D-glucoside, quercetrin), and evaluated oxidative stress and mutagenic/antimutagenic effect of EE and FM through an in vivo experiment using Swiss mice and cyclophosphamide (CP) as an inducer of DNA damage and oxidative stress. Mice were pretreated for 15 consecutive days with EE or FM (250 mg kg-1) and then intraperitoneally injected with CP (25 mg kg-1). Carbonylated proteins, ascorbic acid, catalase and thiobarbituric acid-reactive substances were measured in hepatic and renal tissues. The mutagenic/antimutagenic effect was evaluated through the Micronucleus Test. Protein carbonylation in the liver of animals exposed to CP was reduced by FM. There was no significant effect on other markers of oxidative stress. The groups treated with the extracts showed a significant percentage reduction (EE = 96% and FM = 71%) in the frequency of micronucleated polychromatic erythrocytes induced by CP. EE showed mutagenicity when used alone. The EE and FM of C. decorticans leaves showed antioxidant potential equivalent to that observed in other species, did not cause oxidative stress, nor toxicity, and had a protective and antimutagenic effect, although the EE showed signs of mutagenicity.

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Section: Resultsmentioning

confidence: 99%

First phytochemical and biological study of the ethanolic extract from leaves of Capirona decorticans (Rubiaceae)

et al. 2018

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“…Twenty-four hours after MMC applications, the femoral bones of the mice were removed by cervical dislocation under ether anesthesia. Test concentrations (150 mg/kg and 300 mg/kg) were determined considering the lower and upper limit ranges used in previous studies [24][25][26].…”

Section: Experimental Designmentioning

confidence: 99%

The investigation of the possible antigenotoxic in vivo effects of pomegranate(Punica granatum L.) peel extract on mitomycin-C genotoxicity

Uluman¹,

Kiliçle²

2020

Turk J Vet Anim Sci

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The aim of this study was to investigate the possible antigenotoxic effects of the extract of pomegranate (Punica granatum L.) peel, which has high antioxidant activity against the genotoxic effects that mitomycin-C creates in the bone marrow cells of mice by a chromosomal aberration, mitotic activity, and micronucleus test system. There were six groups including a negative control group, positive control group (2 mg/kg mitomycin-C (MMC)), 150 mg/kg P. granatum L. (PG) peel extract, 300 mg/kg PG peel extract, 150 mg/ kg PG + MMC, and 300 mg/kg PG + MMC. The extract of pomegranate peel was given to mice by oral gavage for 15 days. At the end of day 15, 2 mg/kg MMC was intraperitoneally administered to the mice. Chromosomal aberration, mitotic activity, and micronucleus rates were determined in bone marrow cells of the mice, which were euthanized 24 h after this application. As a result of the observations carried out, it was determined that the chromosomal aberrations and micronucleus ratios in the MMC group were at the maximum level in terms of chromosomal aberration rates and micronucleated polychromatic erythrocytes; this rate decreased with 150 mg/kg PG and 300 mg/kg PG peel extract application with MMC, and PG peel extract applied with MMC significantly increased this decrease due to dose increase (P < 0.001). It was observed that MMC application decreased mitotic activity and polychromatic erythrocyte/ normochromatic erythrocyte ratios, while statistically these rates significantly increased in the groups administered peel extract with MMC when compared to the MMC group (P < 0.001). As a result, it was determined that the peel extract at the stated dose showed antigenotoxic effects against the genotoxicity caused by mitomycin-C in the bone marrow cells of mice.

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“…The pomegranate is an infructescence of the pomegranate (Punica granatum), this species is capable of promoting post-implantation abortion (PROMPROM, et al, 2010;MEMARZADEH, et al, 2015). However, it does not have mutagenic potential (VALADARES et al, 2010;ÁVILA et al, 2013). It is suggested the prenatal intake of pomegranate juice due to its fetoprotective action (CHEN, et al, 2012; CHEN, LONGTINE and NELSON, 2013).…”

Section: Pomegranatementioning

confidence: 99%

Use of Medicinal Plants During the Gestational Period.

Barros¹,

Pernambuco²

2019

IJAR

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Objective: To gather studies on the effects of the use of medicinal plants during the gestational period, as a way of evidencing the effects caused by consumption. Methodology: The articles were searched in the electronic databases PubMed and Sciello. The articles published in the period 2007 to 2017 were included, using the descriptors: "tea", "medicinal plants", "phytotherapy" and "gestation" or "pregnancy", in Portuguese and English. Results: In the search of articles, 819 results were obtained, of which 39 articles were selected, according to the defined inclusion criteria. Conclusion: Information on the safe use of herbal products during pregnancy is still poorly understood, and further studies of plant species that may pose some risk during pregnancy are needed.

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Assessment of mutagenic and antimutagenic effects of Punica granatum in mice

Cited by 23 publications

References 43 publications

First phytochemical and biological study of the ethanolic extract from leaves of Capirona decorticans (Rubiaceae)

First phytochemical and biological study of the ethanolic extract from leaves of Capirona decorticans (Rubiaceae)

The investigation of the possible antigenotoxic in vivo effects of pomegranate(Punica granatum L.) peel extract on mitomycin-C genotoxicity

Use of Medicinal Plants During the Gestational Period.

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