Serological evaluation of Neospora caninum in pregnant women treated at referral center for prenatal screening in Mato Grosso do Sul, Brazil

Duarte, Pâmella Oliveira; Csordas, Bárbara Guimarães; Oshiro, Leandra Marla; Higa, Leandro de Oliveira Souza; Zimmermann, Namor Pinheiro; Martins, Kauê Rodriguez; Barros, Jacqueline Cavalcante; Andreotti, Renato

doi:10.1590/s1984-29612020097

Cited by 4 publications

(5 citation statements)

References 29 publications

(37 reference statements)

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“…Only 7.0% (95% CI: 4.6, 9.4) of women had both anti-T. gondii-IgG and anti-N. caninum IgG (Table 1), which is similar to previous findings of 5.9% and 8.4% seropositivity among pregnant women in Egypt (Ibrahim et al, 2009) and Brazil (Duarte et al, 2020b) respectively.…”

Section: Discussionsupporting

confidence: 88%

“…By contrast, the search for anti-N. caninum-IgM took two attempts using IFAT. One yielded no seropositivity in normally delivered umbilical cord blood (Duarte et al, 2020b) and another in pregnant women reported 5.9% seropositivity at a 1/100 titer (Duarte et al, 2020a).…”

Section: Discussionmentioning

confidence: 88%

“…This was an association between seropositivity of N. caninum in cattle and the presence of dogs (Bartels et al, 1999). As with N. caninum seropositivity in livestock hosts, e.g., cattle and small ruminants (Abo-Shehada & Abu-Halaweh, 2010), and the cord blood of women (Duarte et al, 2020b), N. caninum-IgG seropositivity in RSA women was associated with the presence of dogs in the house (OR = 2.6).…”

Section: Discussionmentioning

confidence: 93%

“…To date, as far as is known, only three risk factors for N. caninum-IgG seropositivity in pregnant women were reported. These are: eating Senegalese barbecued meat (OR = 3.5) (Coulibaly et al, 2020) and the presence of domestic animals (OR = 2.3), and dogs (OR = 2.0) [11] (Duarte et al, 2020b).…”

Section: Discussionmentioning

confidence: 99%

“…5) Four studies employing pregnant women Braz J Vet Parasitol 2021; 30(3): e008821 3/11 Seroprevalence of N. caninum and Toxoplasma gondii in women from Egypt (Ibrahim et al, 2009), Senegal (Coulibaly et al, 2020) and Brazil (Duarte et al, 2020a, b) reported higher seroprevalences of 8%, 14% 23% and 24% respectively. Recently, N. caninum DNA was detected in the umbilical blood of two women in Brazil (Duarte et al, 2020b) raising the concern about the zoonotic potential of N. caninum.…”

Section: Introductionmentioning

confidence: 99%

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Seroprevalences of Toxoplasma gondii and Neospora caninum infections in Jordanian women who had a recent spontaneous abortion

Abo‐Shehada

Khalil

Abu-Halaweh

et al. 2021

Rev. Bras. Parasitol. Vet.

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This cross-sectional study investigates Toxoplasma gondii and Neospora caninum among 445 recently spontaneously aborted (RSA) Jordanian women using ELISA and indirect fluorescent antibody (at a cut-off value of 1/200) tests, respectively. The type of hospital, age, cat and dog contacts, raw and barbecued meat and wild plant consumption, number of abortions, and stillbirths were tested as independent variables using univariate and multivariate logistic regression analyses. The true seroprevalences were 22.1% for T. gondii-IgG, 22.7% for N. caninum-IgG, 2.6% for T. gondii-IgM, 10.6% for N. caninum-IgM, 0% for T. gondii-IgG and IgM, 6.7% for N. caninum-IgG and IgM, and 4.6% and 0% for both parasite IgG and IgM, respectively. T. gondii-IgM-seropositivity was associated with the number of abortions with odds ratios (OR) of 2.4 and eating barbecued meat (OR = 0.12). N. caninum-IgG-seropositivity was associated with having a dog in the house (OR = 2.6), and with stillbirth (OR = 0.1). N. caninum-IgM was associated with visiting a private-hospital (OR = 2.7). RSA Jordanian women are equally exposed to both parasites with significantly (p < 0.05) higher seroprevalence of N. caninum-IgM compared to T. gondii-IgM suggestive of active infections among RSA women in Jordan.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Seroprevalences of Toxoplasma gondii and Neospora caninum infections in Jordanian women who had a recent spontaneous abortion

Abo‐Shehada

Khalil

Abu-Halaweh

et al. 2021

Rev. Bras. Parasitol. Vet.

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Evaluation of a Commercial Serum Competitive Enzyme-Linked Immunosorbent Assay for Detection of Neospora caninum-Specific Antibodies in Raw Milk of Ruminants

Fereig,

Altwaim,

Frey

2024

Parasitologia

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Bovine neosporosis is an infection caused by the protozoan parasite Neospora caninum and has substantial veterinary hazards. Neosporosis cannot be controlled by vaccination or chemotherapy. Thus, accurate diagnosis followed by isolation and culling of infected animals is regarded as the most efficient method of control. In vivo diagnosis often relies on serologic testing of the animals, and milk represents a non-invasive and easy-to-collect sample matrix. However, indirect enzyme-linked immunosorbent assay (ELISA) specifically designed for antibody detection in milk are sometimes not easily available and it is tempting to use ELISA kits that are originally designed for use in serum in milk samples instead. Herein, we evaluated a widely used commercial ELISA (ID Screen® Neospora caninum competition Multispecies ELISA (ID. Vet, Grabels, France)), developed for detection of N. caninum antibodies in serum samples, for its performance on milk samples. Milk samples from dairy ruminants (cows, buffaloes, sheep, and goats; n = 149) were tested in parallel with the serum ELISA and a commercial milk ELISA as a standard test (Neospora caninum Milk Competitive ELISA, ID. Vet, Grabels, France). The detected prevalence values were 28.2% (42/149), 17.4% (26/149), and 17.4% (26/149) using milk ELISA, serum ELISA, and both ELISAs, respectively. Sensitivity, specificity, positive predictive value, and negative predictive value for the serum ELISA used with milk samples were 61.9%, 100%, 100%, and 87%, respectively. The agreement and kappa value between the two ELISAs were 89.3% and 0.70, respectively, suggesting substantial agreement. High values of Pearson correlation coefficient (0.904, p ≥ 0.0001) and area under the receiver operating characteristic (ROC) curve (0.789, p ≥ 0.0001) demonstrated the high diagnostic performance of the serum ELISA in milk samples. Also, a Bland–Altman Plot and histogram describing the frequency of distribution of ELISA optical densities confirmed the high agreement of both serum and milk ELISAs. The current results revealed the high specificity but moderate sensitivity of the serum ELISA used for milk samples compared with the milk ELISA. However, the excellent positive predictive value of the serum ELISA makes it an alternative option in case of the unavailability of milk ELISAs. With this study, we provided additional evidence that a widely used serum ELISA test kit may also be used for the detection of N. caninum antibodies in milk samples.

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Prevalence of Neospora caninum and Toxoplasma gondii Antibodies and DNA in Raw Milk of Various Ruminants in Egypt

Fereig

Abdelbaky²,

Mazeed

et al. 2022

Pathogens

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The prevalence of Neospora caninum and Toxoplasma gondii antibodies in raw milk samples was estimated in different ruminants and Egyptian governorates. Of 13 bulk milk samples tested by ELISA, five (38.5%) were positive for antibodies to N. caninum, and two samples were additionally positive for antibodies to T. gondii, resulting in a seroprevalence of 15.4% for both T. gondii and co-infection. In individual milk samples (n = 171) from the same bulks, antibodies to N. caninum were detected in 25.7%, to T. gondii in 14%, and 3.5% had antibodies to both parasites. A strong correlation between the OD values of the bulk samples and of the relevant individual milk samples was found for T. gondii (Pearson r = 0.9759) and moderately strong for N. caninum (Pearson r = 0.5801). Risk factor assessment for individual milk samples revealed that antibodies to T. gondii were significantly influenced by animal species, while no risk factors were detected for N. caninum antibodies. Additionally, DNA of N. caninum was detected in a bulk milk sample of cattle for the first time in Egypt, and DNA of T. gondii was found in bulk milk samples of cattle, sheep and goats. This is the first study in Egypt in which bulk milk samples of different ruminants were tested for the presence of N. caninum and T. gondii antibodies and DNA. Both individual and bulk milk samples are useful tools for monitoring antibody response to N. caninum and T. gondii infections in different ruminants in Egypt.

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Serological evaluation of Neospora caninum in pregnant women treated at referral center for prenatal screening in Mato Grosso do Sul, Brazil

Cited by 4 publications

References 29 publications

Seroprevalences of Toxoplasma gondii and Neospora caninum infections in Jordanian women who had a recent spontaneous abortion

Seroprevalences of Toxoplasma gondii and Neospora caninum infections in Jordanian women who had a recent spontaneous abortion

Evaluation of a Commercial Serum Competitive Enzyme-Linked Immunosorbent Assay for Detection of Neospora caninum-Specific Antibodies in Raw Milk of Ruminants

Prevalence of Neospora caninum and Toxoplasma gondii Antibodies and DNA in Raw Milk of Various Ruminants in Egypt

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