Molecular detection of Mycoplasma suis in extensive pig production systems in the State of Maranhão, northeast Brazil

Martins, Mickaellen Susanny dos Santos; Silva, Lucas Diniz; Miranda, Leandro Macedo; Lima, Cristian Alex Aquino; Amaral, Renan Bressianini do; Machado, Rosangela Zacarias; André, Marcos Rogério; Braga, Maria do Socorro Costa Oliveira; Rosário, Carla Janaina Rebouças Marques do; Melo, Ferdinan Almeida; Pereira, José Gomes

doi:10.1590/s1984-296120180099

Cited by 3 publications

(6 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Quantitative PCR results showed that more than 80% of the positive animals presented PH loads between 10 − 1 and 10 4 copies/μL, corroborating previous studies conducted in Brazil [ 15 , 17 , 18 , 31 ]. PHs tend to establish chronic infections with nonclinical presentations in pigs [ 6 , 35 ].…”

Section: Discussionsupporting

confidence: 89%

“…Even though PHs have been reported in the intensive [ 18 , 31 ] and extensive [ 15 , 17 ] pig production systems in Brazil, the influence of these agents on productive swine performance has not been assessed. Therefore, this body of work investigated the occurrence of PH and its association with average daily weight gain (ADWG) in finishing pigs from a commercial farm in Brazil.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Porcine hemothropic mycoplasmas infection associated with productive impact in intensive pig production

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background So far, three porcine hemoplasmas (PH) have been identified, namely Mycoplasma suis, Mycoplasma parvum, and Mycoplasma haemosuis. The first one is the main agent associated with porcine hemoplasmosis, a possible cause of economic losses in pig production. Thus, this work aimed to detect and quantify PH 16S rRNA in finishing pigs and to associate its load estimate with average daily weight gain (ADWG). For this purpose, whole blood samples from 318 pigs were collected at an age of 75 days (d0) when the pigs entered the finishing phase and 105 days later (d105). To calculate ADWG, the animals were weighed at the abovementioned dates. Then, DNA from blood samples were submitted to a qPCR targeting the 16S rRNA gene for PH. Spearman correlation test was performed to investigate potential associations between ADWG and the quantification values. Lastly, the molecular characterization of PH was done by sequencing the 23S rDNA gene. Results Out of the 318 samples, 190 (59.74%) were positive on d0, and 304 (95.6%) were positive on d105. A significant correlation was observed (p < 0.05), albeit with a low coefficient value (0.18), when comparing ADWG with quantification values on d105. The phylogenetic analysis based on the 23S rDNA gene showed that four sequences were closely related to M. parvum, and one sequence was positioned in the M. suis cluster. Conclusion Two PH, M. suis and M. parvum, were detected in a Brazilian pig farm. Moreover, increasing occurrence through time was observed, which may have affected the productive performance of positive animals, mainly at the end of the finishing phase, when antimicrobials are removed.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Porcine hemothropic mycoplasmas infection associated with productive impact in intensive pig production

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…(2019) that have found an occurence of 76.19% and 54.7%, respectively. However, both studies (Martins et al., 2019; Toledo et al., 2016) analysed samples from extensive and non‐technified farms, without adequate nutritional programmes, facilities, defined genetics, and standardized management techniques.…”

Section: Discussionmentioning

confidence: 94%

“…Similarly, Martins et al. (2019) detected 54.7% occurrence in extensive pig farms from Rio Grande do Norte State, which raises questions about the situation of HMs occurrence in technified farms in Brazil, the fourth biggest pork producer in the world (United States Department of Agriculture, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…At the genomic level, there are no reports on the diversity of PH affecting pigs in Brazil. Even though M. suis and M. parvum have been detected in domestic and wild pigs from Brazil (Dias et al., 2019; Gatto et al., 2019; Martins et al., 2019; Toledo et al., 2016), and domestic pigs from China (Fu et al., 2017) and Korea (Seo, Kwon, & Kwak, 2019), little is known about the occurrence and genetic diversity of M. parvum . Therefore, cloning and sequencing are important tools used to access the genetic diversity of fastidious agents, using DNA from field samples (Charlebois, Jacques, & Archambault, 2014).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic diversity of emerging hemotropic mycoplasmas in domestic pigs from Brazil

Sonálio

Perles

Gatto

et al. 2020

Transbound Emerg Dis

View full text Add to dashboard Cite

Mycoplasma suis and Mycoplasma parvum bind strongly to erythrocytes and may cause clinical hemoplasmosis in swine, affecting several age groups. Mycoplasma spp. infected animals may be asymptomatic carriers and/or show nonspecific clinical signs.In Brazil, information on genetic diversity associated with porcine hemoplasmas (PH) has not been described yet. Therefore, this study has aimed to detect, quantify and characterize the genetic diversity of PH in finishing pigs from technified farms in the state of Goiás, central-western Brazil. Ethylenediaminetetraacetic acid-blood samples from 450 swine belonging to 30 different farms from Goiás state were collected at the slaughterhouse. Quantitative real-time PCR (qPCR) assays were performed for the molecular detection and quantification of PH 16S rRNA gene fragments. Cloning and sequencing of 16S and 23S rRNA amplicons were performed to evaluate the genetic diversity. Moreover, a questionnaire was applied to each farm manager to obtain epidemiological information about the herd. The results on qPCR showed herd occurrence of 68.89% for PH. Quantification values (starting quantity [SQ]) ranged from 8.43 × 10 −1 to 4.69 × 10 6 copies/µl, and 52.71% of the samples presented SQ values equal or lower than 1 × 10 3 copies/µl. Risk factors were not evaluated once all farms had at least one positive animal. However, Spearman's coefficient test revealed that the occurrence of PH was inversely associated with the number of farrows per week, weaned piglets per week, and weight at slaughter. Phylogenetic analysis based on maximum likelihood and Bayesian methods showed that the 16S rRNA and 23S rRNA gene sequences obtained from five samples formed a single cluster closely related to M. parvum. Genotype analysis using DNASP software confirmed seven and four different 16S and 23S rRNA genotypes among the cloned amplicons, indicating that there are several genotypes of M. parvum circulating in individual pigs and among pig farms in central-western Brazil. K E Y W O R D S emerging disease, genotypes, intensive pig farming, Mycoplasma parvum, Mycoplasma suis, porcine hemoplasmas | 1163 SONALIO et AL. | INTRODUC TI ONHemotropic mycoplasmas (HMs) are known for infecting several mammal species, including wild and domestic pigs. Three hemoplasmas have been described in swine, namely Mycoplasma suis (Kinsley, 1932), Mycoplasma parvum (Splitter, 1950) and 'Candidatus M. haemosuis ' (Fu et al., 2017). The above-mentioned HMs induce red blood cells (RBCs) to undergo programmed cell death, also known as eryptosis, characterized by cell shrinkage, membrane blebbing, activation of proteases and phosphatidylserine exposure on the outer membrane leading to recognition by macrophages and, therefore, phagocytosis (Felder

show abstract

Molecular detection and genetic analysis of porcine haemoplasmas in commercial pig farms from Thailand reveal a putative novel species

Thongmeesee

Kamkong

Thanee

et al. 2022

Transbounding Emerging Dis

View full text Add to dashboard Cite

Haemoplasma is a trivial name for haemotropic Mycoplasma spp., which can attach to the surface of red blood cells leading to deformity and anaemia in a wide range of mammalian animals, including pigs. In Thailand, there is only one study that reported the occurrence of Mycoplasma suis without other haemoplasma species. In this study, we examined the molecular occurrence and genetic diversity of porcine haemoplasmas in Thai domestic pigs (Sus scrofa domesticus) from commercial farms using a PCR assay targeting the 16S rRNA gene, DNA sequencing, nucleotide sequence type (ntST) analysis and phylogenetic analysis. A total of 665 blood samples were collected from pigs at thirteen farms located in eight provinces of Thailand during 2019–2020. Genomic DNA was extracted from blood samples and tested by PCR. The frequency of haemoplasma infection was 37.1% (247/665, 95% CI: 33.5%–40.9%) in all pigs. Among 247 PCR positive samples, 194 were sequenced and analysed by nucleotide BLAST, ntST diversity, phylogenetic trees and ntST networks. The results of this genetic analysis indicated that at least four species with 27 nucleotide sequence types (Mycoplasma suis, Mycoplasma parvum, Candidatus Mycoplasma haemosuis and a putative novel species) of porcine haemoplasmas were identified. Thus, it appears that haemoplasmas show a high genetic diversity in the Thai pig population. In addition, a putative novel species was genetically characterized by other markers, namely, the 23S rRNA and RNase P RNA (rnpB) genes. For phylogenetic analysis, Candidatus Mycoplasma haemosuis was placed into the Mycoplasma haemofelis group, and the three remaining species were placed into the Mycoplasma suis group in all trees containing the 16S rRNA, 23S rRNA and rnpB genes. Further studies, such as pathobiology and epidemiology, should be conducted to better characterize this putative novel species.

show abstract

Molecular detection of Mycoplasma suis in extensive pig production systems in the State of Maranhão, northeast Brazil

Cited by 3 publications

References 13 publications

Porcine hemothropic mycoplasmas infection associated with productive impact in intensive pig production

Porcine hemothropic mycoplasmas infection associated with productive impact in intensive pig production

Genetic diversity of emerging hemotropic mycoplasmas in domestic pigs from Brazil

Molecular detection and genetic analysis of porcine haemoplasmas in commercial pig farms from Thailand reveal a putative novel species

Contact Info

Product

Resources

About