Predatory activity of Butlerius nematodes and nematophagous fungi against Haemonchus contortus infective larvae

Silva, Manoel Eduardo da; Uriostegui, Miguel Angel Mercado; Millán-Orozco, Jair; Gives, Pedro Mendoza de; Hernández, Enrique Liébano; Braga, Fábio Ribeiro; Araújo, Jackson Víctor de

doi:10.1590/s1984-29612016091

Cited by 14 publications

(9 citation statements)

References 16 publications

(14 reference statements)

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“…In an extensive review of the available literature, only a few records on the predatory activity of predatory nematodes on animal parasitic nematodes were found relating to Butlerius sp. [7,20,25]. It is interesting that in a similar experiment published by da Silva et al [7], the interaction between Butlerius sp.…”

Section: Discussionmentioning

confidence: 84%

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Butlerius butleri (Nematoda: Diplogasteridae) Feeds on Haemonchus contortus (Nematoda: Trichostrongylidae) Infective Larvae and Free-Living Nematodes in Sheep Faecal Cultures Under Laboratory Conditions: Preliminary Report

et al. 2020

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Aim This study assessed the feeding activity of the predatory nematode Butlerius butleri on the infective larvae of the sheep parasitic nematode Haemonchus contortus (Hc-L 3 ) and on two free-living nematodes Panagrellus redivivus (Pr) and Rhabditis sp. (R) in sterile sheep faecal cultures. Materials and Methods A bioassay was carried out in sheep faecal cultures, where predatory and prey nematodes were put together to assess the predatory behaviour on the different nematodes. Faecal cultures (n = 9) were set up in different treatments as follows: Treatments 1, 2 and 3 contained 20 specimens of B. butleri and 2000 Hc-L 3 , Pr and R, respectively; treatments 4, 5 and 6, contained only 2000 Hc-L 3 , Pr and R, respectively, as control groups. Treatment 7 contained 20 B. butleri as control. All treatments were incubated at room temperature for 30 days. The average number of nematodes recovered from each treatment was obtained and the reduction rate with respect to their corresponding control was estimated. Data were analysed using an ANOVA test using a completely randomised design in the SAS programme. Results Butlerius butleri reduced the Hc-L 3 , population by 91.8%, whereas its population increased 131.6 times. The interactions between B. butleri and Pr and R resulted in 80.9% and 62.5% nematode reduction and B. butleri increased its populations 268.3 and 82.7 times, respectively. This experiment was performed only once, so this can be considered a preliminary report at this time. Conclusion This study provides evidence for the first time that B. butleri feeds on one of the most economically important nematodes of small ruminants, Hc-L 3 .

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Section: Discussionmentioning

confidence: 84%

“…Nematodes of the different taxa groups were recovered transferring the faecal culture material using the Baermann funnel technique [7]. Quantification of nematodes was carried out by taking ten 5-μL aliquot drops from a known volume of an aqueous nematode suspension from the Baermann funnel [15].…”

Section: Nematode Recoverymentioning

confidence: 99%

Butlerius butleri (Nematoda: Diplogasteridae) Feeds on Haemonchus contortus (Nematoda: Trichostrongylidae) Infective Larvae and Free-Living Nematodes in Sheep Faecal Cultures Under Laboratory Conditions: Preliminary Report

et al. 2020

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“…The nematode predatory action of the fungi D. flagrans and M. thaumasium has been extensively studied and proven in the literature (BRAGA & ARAÚJO, 2014;BRAGA et al, 2009;LUNS et al, 2018;SILVA et al, 2017b). However, to the best of our knowledge, this is the first report to compare the activity of them over seven days of interactions on Rhabditis spp.…”

Section: Daysmentioning

confidence: 99%

Rhabditis spp., in the Espírito Santo, State of Brazil and evaluation of biological control

Sobral

Ferreira

Senna

et al. 2019

Rev. Bras. Parasitol. Vet.

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The objectives of this study were to describe occurrences of Rhabditis spp. causing parasitic otitis in dairy cattle of Gir breed in the state of Espírito Santo, southeastern Brazil, and to evaluate the biological control of this nematode using the nematophagous fungi Duddingtonia flagrans (AC001) and Monacrosporium thaumasium (NF34). After nematode detection and collection, three groups were formed: two groups that were treated, respectively, with the fungal isolates; and a control group, without fungus. The treatments were as follows: (a) Petri dishes containing the culture medium 2% water agar (WA) + 250 nematodes + AC001; (b) Petri dishes containing 2% WA + 250 nematodes + NF34; and (c) Petri dishes containing only 2% WA + 250 nematodes. After seven days at 27 °C the treatments with fungi were able to capture and destroy the nematodes, with percentages of 82.0% (AC001) and 39.0% (NF34) in relation to the control group. The results demonstrate the occurrence of Rhabditis spp. after animals physical examination and that there was efficacy of the in vitro predatory activity of both fungal isolates. Thus, these results are important because they can assist in future in vivo control of this nematode in cattle.

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“…Likewise, A. musiformis has also demonstrated biological activity against nematodes of importance in the sheep industry. A recent study (SILVA et al, 2017) reported an important reduction (>90%) in the H. contortus infective larvae population in microplots with Panicum spp. grass with the addition of D. flagrans conidia.…”

Section: Introductionmentioning

confidence: 98%

In vitro predatory activity of nematophagous fungi isolated from water buffalo feces and from soil in the Mexican southeastern

Ojeda-Robertos

Aguilar-Marcelino²,

Olmedo-Juárez³

et al. 2019

Rev. Bras. Parasitol. Vet.

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Nematophagous fungi from the feces of water buffalo and soil from southeastern Mexico were isolated, and their in vitro predatory activity against Haemonchus contortus infective larvae (L3) (HcL3) was assessed. The fungi were isolated by sprinkling soil or feces on water agar plates. Six series of 10 Petri dishes containing a 7-day-old culture of each fungus and a series without fungi as the control were prepared. Five hundred HcL3 were added to each plate. The plates were incubated at room temperature. The average of recovered HcL3 was considered to estimate the larval reduction rate. Four nematophagous fungi isolates corresponding to Arthrobotrys oligospora, var microspora (strains 4-276, 269 and 50-80) and one identified as A. oligospora,var. oligospora (isolates 48-80) were obtained from water buffalo feces. From the soil, five isolates were isolated; three corresponded to A. musiformis (Bajío, Yumca and Macuspana isolates), and two isolates were identified as A. oligospora (Comalcalco and Jalapa de Méndez isolates). The predatory activity of isolates from water buffalo feces ranged between 85.9 and 100%. Meanwhile, the fungi from the soil ranged between 55.5 and 100% (p≤0.05). The nematophagous fungi obtained could have important implications in the control of parasites of importance in the livestock industry.

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Predatory activity of Butlerius nematodes and nematophagous fungi against Haemonchus contortus infective larvae

Cited by 14 publications

References 16 publications

Butlerius butleri (Nematoda: Diplogasteridae) Feeds on Haemonchus contortus (Nematoda: Trichostrongylidae) Infective Larvae and Free-Living Nematodes in Sheep Faecal Cultures Under Laboratory Conditions: Preliminary Report

Butlerius butleri (Nematoda: Diplogasteridae) Feeds on Haemonchus contortus (Nematoda: Trichostrongylidae) Infective Larvae and Free-Living Nematodes in Sheep Faecal Cultures Under Laboratory Conditions: Preliminary Report

Rhabditis spp., in the Espírito Santo, State of Brazil and evaluation of biological control

In vitro predatory activity of nematophagous fungi isolated from water buffalo feces and from soil in the Mexican southeastern

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