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Pleurotus djamor is an edible mushroom that has medicinal properties. This study aimed to assess the in vitro activity of P. djamor extracts and fractions against Haemonchus contortus eggs and exsheathed infective larvae (L). Crude hydroalcoholic extracts were obtained by maceration and fractions were obtained through chromatography. Metabolite identity was determined using gas chromatography/mass spectrometry (GC-MS) analysis. The results showed that P. djamor extracts had no significant activity against eggs at the concentrations used. However, the extract showed 98.7% and 77% larval mortality 72 h postconfrontation at 320 and at 160 mg/mL, respectively. The chromatography analysis resulted in 23 fractions that were eventually grouped into three fractions (E1, E2, and E3). These fractions showed the following egg hatching inhibition percentages: E1 = 100, E2 = 38.7, and E3 = 5.5 at 10 mg/mL concentration 72 h postexposure. Likewise, larval mortality percentages after this period were 90.6, 100, and 0.44 at 40 mg/mL (P < .05), respectively. The GC-MS showed five major compounds in E1 fraction, including four fatty acids: (i) pentadecanoic, (ii) hexadecanoic, (iii) octadecadienoic, (iv) octadecanoic acid, and one terpene identified as β-sitosterol. We concluded that the edible mushroom P. djamor possesses nematicidal metabolites, which could be used as an alternative anthelmintic treatment.

This review describes the advances acquired and proven in the use of helminthophagous fungi in the control of gastrointestinal helminth parasites in domestic animals. Old and well-known premises about parasitic epidemiology and the factors that can interfere with the best performance of biological control are mentioned. Some of the most promising fungi are Duddingtonia flagrans from the predatory fungi group and Pochonia chamydosporia and Mucor circinelloides from the ovicidal fungi group. These fungi produce resistance spores called chlamidospores. Bioverm® and BioWorma®, based on the fungus D. flagrans, are available as commercial. Biotechnological products such as nanoparticles and obtaining primary and secondary metabolites have already been obtained from these fungi. Because they have different mechanisms of action, ovicidal and predatory fungi, when used together, can present a complementary and synergistic action in the biological control of helminths. Therefore, future research in the search for new formulations, the association of fungi from different groups, extraction of new molecules, and nanoparticles of these fungi in the control of helminths in various domestic animals are desired.

BackgroundThe use of Duddingtonia flagrans as a tool for the biological control of gastrointestinal nematodes (GIN) is a promising alternative to anthelmintics. The chlamydospores of D. flagrans are orally dosed and their thick cell wall gives them the capacity to resist digestion and pass through the gastrointestinal tract (GIT). Chlamydospores reaching the faeces are able to germinate and trap nematode larvae. The efficacy of this control method is based on reducing the numbers of infective larvae leaving the faeces. Techniques have recently been developed for quantifying the numbers of chlamydospores in faeces. As the number of non-digested spores could be relevant in the design and optimization of dosing programmes for the control of GIN infective larvae, the aim of the present study was to estimate the loss of D. flagrans chlamydospores during their passage through the ruminant gastrointestinal tract using in vitro and in vivo techniques.ResultsAfter in vitro rumen digestion, chlamydospore recovery was not different from the quantity originally incubated (undigested spores) (P > 0.05). In vitro rumen+abomasum digestion caused nearly 36% loss of the chlamydospores originally incubated (P < 0.05). Germination of chlamydospores classified as viable was 24.3%. Chlamydospores classified as non-viable did not germinate. Rumen digestion resulted in more spore germination (R1 = 35.7% and R2 = 53.3%) compared to no digestion (time 0 h = 8.7%). Subsequent abomasal digestion reduced germination (R1+A = 25%) or stopped it (R2+A = 0%). In vivo apparent chlamydospore digestibility in sheep showed a loss of 89.7% of the chlamydospores (P < 0.05).ConclusionsThe loss of chlamydospores was evident under in vitro and in vivo conditions. Negligible amounts of spores were lost during the in vitro rumen digestion. However, in vitro rumen+abomasum digestion resulted in a chlamydospore loss of approximately 36%. In vivo passage through the sheep GIT resulted in a total loss of 89.7% of the orally administered spores.

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