A new laboratorial method for the diagnosis of gastrointestinal parasites in dogs

Coelho, Willian Marinho Dourado; Gomes, Jancarlo Ferreira; Amarante, Alessandro Francisco Talamini do; Bresciani, Kátia Denise Saraiva; Lumina, Giuliano; Koshino-Shimizu, Sumie; Leme, Denise Pereira; Falcão, Alexandre X.

doi:10.1590/s1984-29612013000100002

Cited by 15 publications

(17 citation statements)

References 20 publications

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“…The precursor technique of this study, called TF-Test (Three Fecal Test), has been used with practicality for years for fecal parasitological diagnosis in humans, with excellent results (Gomes et al, 2004;Carvalho et al, 2012Carvalho et al, , 2016. More recently, this technique has been extended to research in animal area by studies involving sheep (Lumina et al, 2006) and canine (Coelho et al, 2013(Coelho et al, , 2015 species. In these works, new diagnostic procedures were validated with gastrointestinal helminths and protozoa, not taking into account the genus Cryptosporidium.…”

Section: Introductionmentioning

confidence: 99%

Validation of a new technique to detect Cryptosporidium spp. oocysts in bovine feces

Inácio

Gomes

Oliveira

et al. 2016

Preventive Veterinary Medicine

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Due to its important zoonotic potential, cryptosporidiosis arouses strong interest in the scientific community, because, it was initially considered a rare and opportunistic disease. The parasitological diagnosis of the causative agent of this disease, the protozoan Cryptosporidium spp., requires the use of specific techniques of concentration and permanent staining, which are laborious and costly, and are difficult to use in routine laboratory tests. In view of the above, we conducted the feasibility, development, evaluation and intralaboratory validation of a new parasitological technique for analysis in optical microscopy of Cryptosporidium spp. oocysts, called TF-Test Coccidia, using fecal samples from calves from the city of Araçatuba, São Paulo. To confirm the aforementioned parasite and prove the diagnostic efficiency of the new technique, we used two established methodologies in the scientific literature: parasite concentration by centrifugal sedimentation and negative staining with malachite green (CSN-Malachite) and Nested-PCR. We observed good effectiveness of the TF-Test Coccidia technique, being statistically equivalent to CSN-Malachite. Thus, we verified the effectiveness of the TF-Test Coccidia parasitological technique for the detection of Cryptosporidium spp. oocysts and observed good concentration and morphology of the parasite, with a low amount of debris in the fecal smear.

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Section: Introductionmentioning

confidence: 99%

Validation of a new technique to detect Cryptosporidium spp. oocysts in bovine feces

Inácio

Gomes

Oliveira

et al. 2016

Preventive Veterinary Medicine

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“…Environmental changes caused by humans and their interaction with domestic animals, particularly with dogs, have favored the occurrence of various parasitic diseases with zoonotic potential (BRESCIANI et al, 2008;COELHO et al, 2013). This scenario has led to a high worldwide prevalence of canine gastrointestinal parasites, especially in the tropical regions of underdeveloped and developing countries (CHOMEL, 2008;COELHO et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Numerous studies on dog feces describe discrepancies in the diagnostic efficacy of different parasitological techniques and/or commercial kits (TÁPARO et al, 2006;COELHO et al, 2013). In view of this finding, some researchers recommend the collection of triplicate fecal samples, on alternate days, in combination with the high parasite concentration technique for this same diagnostic modality (GOMES et al, 2004;CARVALHO et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Comparative study of five techniques for the diagnosis of canine gastrointestinal parasites

Coelho

Gomes

Falcão

et al. 2015

Rev. Bras. Parasitol. Vet.

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Differences in the efficacy of diagnostic techniques employed in the parasitological examination of feces are a limiting factor of this laboratory procedure in the field of Veterinary Parasitology. To verify advances in this type of examination in dogs, we conducted a study using a new technique (TFGII/Dog). Fifty naturally infected dogs were housed in individual stalls, and their feces were evaluated comparatively using this technique and four other conventional techniques. The TFGII/Dog showed high levels of sensitivity and efficiency, surpassing the diagnostic accuracy of the other techniques with a kappa concordance index of 0.739 (Substantial), as opposed to 0.546 (Moderate), 0.485 (Moderate), 0.467 (Moderate), and 0.325 (Fair) of the Spontaneous-Sedimentation, Centrifugal-Flotation in Saturated Zinc Sulfate Solution, Centrifugal-Flotation in Saturated Sugar Solution, and Spontaneous-Flotation in Saturated Sodium Chloride Solution techniques, respectively. The combination of positive results of all techniques comprises eight genera of parasites, with Ancylostoma spp. predominating among helminths, and Cystoisospora spp. among protozoa. The TFGII/Dog technique showed better diagnostic performance, and can therefore be considered an important tool for optimizing the results of laboratory routines and for the control of canine gastrointestinal parasites.

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“…Souza-Dantas et al (2007) demonstrated the importance of using the Faust technique, which reached 100% sensitivity in comparison to necropsy of the intestinal tract of cats. Coelho et al (2013) also obtained a good performance using Faust compared in other techniques used in its diagnosis of Ancylostomatidae.…”

Section: Resultsmentioning

confidence: 85%

Analysis of the accuracy of different laboratory methods for the diagnosis of intestinal parasites from stray and domiciled cats (Felis catus domesticus) in Goiânia, Goiás, Brazil

Lima

Rezende

Rocha

et al. 2018

Rev. Bras. Parasitol. Vet.

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Cats are carriers of zoonotic agents to humans, including intestinal parasites. The purpose of this study was to analyze the accuracy of different laboratory methods for the diagnosis of intestinal parasites. Fecal samples were processed by the Willis, Sheather, Faust and Hoffman-Janer-Pons-Lutz (HJPL) methods. Accuracy analysis was performed determining the sensitivity, specificity, positive and negative predictive value and Kappa. A total of 149 fecal samples were collected, 65 from stray cats and 84 from domiciled cats. The prevalence of intestinal parasites in stray cats was 60% while in domiciled cats it was 17%. In the analysis of accuracy, the techniques that showed the greatest accuracy for Ancylostomids were Willis and Faust, for Cystoisospora spp. Sheather with Faust or HPJL, and Toxoplasma gondii/Hammondia hammondi the association between Willis and Faust. Therefore, for a reliable evaluation of the prevalence of intestinal parasites, at least two different techniques should be used in parasitological exams of feces.

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A new laboratorial method for the diagnosis of gastrointestinal parasites in dogs

Abstract: In

Cited by 15 publications

References 20 publications

Validation of a new technique to detect Cryptosporidium spp. oocysts in bovine feces

Validation of a new technique to detect Cryptosporidium spp. oocysts in bovine feces

Comparative study of five techniques for the diagnosis of canine gastrointestinal parasites

Analysis of the accuracy of different laboratory methods for the diagnosis of intestinal parasites from stray and domiciled cats (Felis catus domesticus) in Goiânia, Goiás, Brazil

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