Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblasts

Isaac, César; Paggiaro, André Oliveira; Aldunate, Johnny Leandro Conduta Borda; Herson, Marisa Roma; Altran, Silvana Cereijido; Beatriz, Mathor Mônica; Ferreira, Marcus Castro

doi:10.1590/s1983-51752011000300007

Cited by 7 publications

(3 citation statements)

References 16 publications

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“…Transitions of these different cellular states of keratinocytes are modulated by various wound microenvironmental cues, including growth factors, cytokines, chemokines, and matrix metalloproteinases (MMPs), which have been extensively reviewed previously [ 2 ]. Moreover, keratinocytes participate in contracting wounds together with fibroblasts [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

The Immune Functions of Keratinocytes in Skin Wound Healing

Piipponen

Landén

2020

IJMS

241

139

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As the most dominant cell type in the skin, keratinocytes play critical roles in wound repair not only as structural cells but also exerting important immune functions. This review focuses on the communications between keratinocytes and immune cells in wound healing, which are mediated by various cytokines, chemokines, and extracellular vesicles. Keratinocytes can also directly interact with T cells via antigen presentation. Moreover, keratinocytes produce antimicrobial peptides that can directly kill the invading pathogens and contribute to wound repair in many aspects. We also reviewed the epigenetic mechanisms known to regulate keratinocyte immune functions, including histone modifications, non-protein-coding RNAs (e.g., microRNAs, and long noncoding RNAs), and chromatin dynamics. Lastly, we summarized the current evidence on the dysregulated immune functions of keratinocytes in chronic nonhealing wounds. Based on their crucial immune functions in skin wound healing, we propose that keratinocytes significantly contribute to the pathogenesis of chronic wound inflammation. We hope this review will trigger an interest in investigating the immune roles of keratinocytes in chronic wound pathology, which may open up new avenues for developing innovative wound treatments.

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Section: Introductionmentioning

confidence: 99%

The Immune Functions of Keratinocytes in Skin Wound Healing

Piipponen

Landén

2020

IJMS

241

139

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“…These include organotypic skin constructs, the use of conditioned media, and indirect contact using transwell membrane inserts [12,[16][17][18]. A significant contraction has been shown in murine type I collagen gels populated with NFs upon keratinocytes adding to the gel compared to the control group (no keratinocytes) [19]. Moreover, a greater contraction was observed in collagen lattice populated with KFs compared to NFs populated lattices.…”

Section: Introductionmentioning

confidence: 99%

Secreted Factors from Keloid Keratinocytes Modulate Collagen Deposition by Fibroblasts from Normal and Fibrotic Tissue: A Pilot Study

et al. 2020

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Interactions between keratinocytes and fibroblasts in the skin layers are crucial in normal tissue development, wound healing, and scarring. This study has investigated the role of keloid keratinocytes in regulating collagen production by primary fibroblasts in vitro. Keloid cells were obtained from removed patients’ tissue whereas normal skin cells were discarded tissue obtained from elective surgery procedures. Fibroblasts and keratinocytes were isolated, cultured, and a transwell co-culture system were used to investigate the effect of keratinocytes on collagen production using a ‘scar-in-a-jar’ model. Keloid fibroblasts produced significantly more collagen than normal skin fibroblasts in monoculture at the RNA, secreted protein, and stable fibrillar protein level. When keloid keratinocytes were added to normal skin fibroblasts, expression of collagen was significantly upregulated in most samples, but when added to keloid fibroblasts, collagen I production was significantly reduced. Interestingly, keloid keratinocytes appear to decrease collagen production by keloid fibroblasts. This suggests that signaling in both keratinocytes and fibroblasts is disrupted in keloid pathology.

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“…A successful wound closure requires a crosstalk between the keratinocytes and other cell types involved in wound healing [1]. Keratinocytes may also play a role in the extracellular matrix reorganization [2].…”

Section: Introductionmentioning

confidence: 99%

Influence of traditional medicines on the activity of keratinocytes in wound healing: an in-vitro study

et al. 2019

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Natural medicinal systems such as Ayurveda and folk medicine has remedies for wound management. However, the exact cellular and extracellular mechanisms involved in the healing process and its influence on keratinocytes is less discussed. Therefore, the present study was designed to evaluate the effect of certain natural wound healing medicines on the biology of the keratinocytes/HaCaT cells. Test materials such as honey (H), ghee (G), aqueous extracts of roots of Glycyrrhiza glabra (GG) and leaves of Nerium indicum (NI) were considered. The HaCaT cells were treated with the test materials singly and in combinations (H+G, all combined [Tot]) for a specific period (24, 48, and 72 hours). The cells were then subjected to cytotoxicity/proliferation and migration/scratch assays. All the test materials, except NI, were non-cytotoxic and showed increased cell proliferation at variable concentrations. Significant observations were made in the groups treated with honey

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Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblasts

Cited by 7 publications

References 16 publications

The Immune Functions of Keratinocytes in Skin Wound Healing

The Immune Functions of Keratinocytes in Skin Wound Healing

Secreted Factors from Keloid Keratinocytes Modulate Collagen Deposition by Fibroblasts from Normal and Fibrotic Tissue: A Pilot Study

Influence of traditional medicines on the activity of keratinocytes in wound healing: an in-vitro study

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