Induction of suppressiveness to Fusarium wilt of chrysanthemum with composted sewage sludge

Abstract

The effectiveness of composted sewage sludge incorporated into Pinus bark-based substrate with or without biofertilizer, fish hydrolyzate, chitosan and Trichoderma asperellum was evaluated for the control of Fusarium wilt in chrysanthemum. The substrate was obtained from pots containing chrysanthemum plants killed by the pathogen. Half of the substrate was sterilized prior to the incorporation of sewage sludge (0, 10%, 20% and 30% v/v). These substrates were or were not supplemented with the following: biofertilizer, fish hydrolyzate and Trichoderma. The mixtures were transferred to pots, and the chrysanthemum was transplanted. For all treatments, half of the plants were sprayed weekly with chitosan. Assessment of severity was performed on the 8 th , 12 th , 15 th and 20 th week after transplanting. In the 12 th week, microbiological and chemical analysis of the substrate was performed. The incorporation of composted sewage sludge into the Pinus bark-based substrate significantly reduced Fusarium wilt, which was progressively decreased as the concentration of sewage sludge increased. The addition of biofertilizer, fish hydrolyzate, chitosan and Trichoderma had no effect on the disease. The microbial community was greater in non-disinfested substrates. The results indicate that suppressiveness is related to the interaction of chemical and microbiological factors.

Combined Effects of Soil Biotic and Abiotic Factors, Influenced by Sewage Sludge Incorporation, on the Incidence of Corn Stalk Rot

“…High dosages of sewage sludge were applied in the present study to simulate the cumulative effect of a long-term incorporation of repeated soil application of this waste. However, higher dosages were used in others studies [ 11 , 12 , 15 , 54 ].…”

“…cucumerinum [ 14 ]; on chrysanthemum, caused by Fusarium oxysporum f. sp. chrysanthemi [ 15 ] and on tomato, caused by Fusarium oxysporum f. sp. lycopersici [ 16 ].…”

Metabolic response induced by endophytic fungi and bacteria in H. marrubioides Epling in vitro microplants

“…28 The genus Papulaspora has also been shown to display antimicrobial activity 29 and potential for secondary metabolites production. 30 On the other hand, the genus Fusarium is known to trigger wilting, or Fusariosis, characterized by xylem hypertrophy 31 and wilting followed by death in affected plants. 32 These symptoms were detected in plants submitted to treatment T7 (RG32), which underwent inoculation with Fusarium (Figure 1).…”

Manejo nutricional integrado: herramienta clave para la agricultura sostenible

“…Por ejemplo, en un ensayo de tizón tardío en tomate se registró menor incidencia (13.8%) con 75% FM + Vc (4 t ha -1 ) + biofertilizante en comparación con la FM sola (31.6%) (Chatterjee y Khalko, 2013). En crisantemo, Pinto et al (2013) Estudiaron el efecto del MNI con diversos AOs sobre la supresión de Fusarium y encontraron que la supresión fue mayor en la medida que se elevó la dosis de Cp, pero el adicionar biofertilizante, hidrolizado de pescado, quitosano o Trichoderma, no tuvo efecto sobre la enfermedad, lo anterior sugiere que la supresión depende de la interacción de factores químicos y microbiológicos diversos.…”