Potencial da microbiolização de sementes de arroz com rizobactérias para o biocontrole do nematoide das galhas

Ludwig, Juliane; Moura, Andrea Bittencourt; Gomes, C. B.

doi:10.1590/s1982-56762013005000007

Cited by 22 publications

(16 citation statements)

References 20 publications

(14 reference statements)

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“…All they changed the color of the medium, from yellow to reddish, because of the pH increase caused by the final products of urea breaks generated by the action of urease (Table 2). These characteristics are similar to those observed at the genera Azospirillum, Pseudomonas and Bacillus (FERREIRA; KNUPP; MARTIN; DIDONET, 2014;SILVA, 2013;MANDELBAUM;ALLAN;WACKETT, 1995).…”

Section: Phenotypical Evaluations Of the Isolatessupporting

confidence: 78%

ISOLATION AND CHARACTERIZATION OF PLANT GROWTH PROMOTING BACTERIA ISOLATED FROM GARLIC (allium sativum).

Botelho¹,

Leoncio

2017

RSA

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-Garlic has great economic and social importance in Santa Catarina. It is a demanding crop in nutrients, being necessary high amount of chemical fertilizers, increasing the cost of production and the environmental impact. An alternative to the use of inputs are Rizobacteria Promoting Plant Growth (RPCP). Among the RPCPs, the most studied genera are Azospirillum, Bacillus and Pseudomonas of the fluorescent group that have shown benefits for the growth of several plant species. In this context, isolates of RPCP obtained from the rhizosphere of garlic maintained in protected cultivation were phenotypically and genotypically characterized and a growth promotion test in vivo was carried out, in protected cultivation. The biochemical tests used for phenotypic characterization were glucose and sucrose fermentation, Methyl Red (MR), catalase production and urea hydrolysis. The potential of plant growth induction was also evaluated by analyzing the production capacity of IAA (Indol-Acetic Acid) and calcium phosphate solubilization in vitro. Twenty-seven isolates were obtained. For the biochemical tests, 21 isolates were able to ferment glucose and 26, sucrose. For the other tests, all isolates showed a positive reaction. As for the mechanisms of plant growth promotion, 81.5% and 66.6% of the isolates presented IAA production and phosphate solubilizing capacity, respectively. The in vivo growth promotion test using maize (Zea mays) as a model indicated that the tested isolates (EB02, EB16, EB14, EB26, EB23) showed similarity to the treatment with 100% recommended nitrogen fertilization. The isolates were identified by sequencing the 16S rRNA gene. Fourteen isolates (51, 85% of the collection) were identified as Bacillus subtillis, including those tested in planta. These microorganisms present potential for the promotion of plant growth.Key words: growth promotion, rizobacteria, liliacea, corn.Resumo -O alho possui grande importância econômica e social em Santa Catarina. É um cultivo exigente em nutrientes, sendo necessária elevada quantidade de fertilizantes químicos, incrementando o custo de produção e o impacto ambiental. Uma alternativa ao uso de insumos são as Rizobactérias Promotoras de Crescimento de Plantas (RPCP). Dentre as RPCPs, os gêneros mais estudados são Azospirillum, Bacillus e Pseudomonas do grupo fluorescente que têm demonstrado benefícios para o crescimento de várias espécies vegetais. Neste contexto, foram caracterizados, fenotípica e genotipicamente, isolados de RPCP obtidos da rizosfera de alho mantido em cultivo protegido e realizado teste de promoção de crescimento in vivo, também em cultivo protegido. Os testes bioquímicos utilizados para caracterização fenotípica foram fermentação de glicose e sacarose, Vermelho de Metila (VM), produção de catalase e hidrólise da ureia. Também foi avaliado o potencial de indução do crescimento vegetal, mediante análise da capacidade de produção de AIA (Ácido Indol-Acético) e de solubilização de fosfatos de cálcio in vitro. Foram obtidos 27 isolados. Para ...

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Section: Phenotypical Evaluations Of the Isolatessupporting

confidence: 78%

ISOLATION AND CHARACTERIZATION OF PLANT GROWTH PROMOTING BACTERIA ISOLATED FROM GARLIC (allium sativum).

Botelho¹,

Leoncio

2017

RSA

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“…This bacterium, as well as DFs185, belongs to a well-studied group of biological control agents, which makes it possible to suggest that these isolates act by antibiosis for the control of these pathogens. This possibility is supported by the fact that these isolates, in addition to presenting antifungal activity against the fungi used in this study, also act by antibiosis against other rice pathogens, such as Alternaria lunata, Curvularia lunata, R. solani (Ludwig and Moura, 2009) and M. graminicola (Ludwig et al, 2013).…”

Section: Figure 1 Relative Percentage Of Gerlachia Oryzae Andmentioning

confidence: 58%

“…On the other hand, the possibility of isolate DFs185 also acting by resistance induction is not ruled out, even knowing its in vitro antibiosis capacity. The possible occurrence of induction by both bacteria is based on the fact that when they were used in other studies, it was observed control of Meloidogyne graminicola (Ludwig et al, 2013) and R. solani (Ludwig and Moura, 2007), therefore exhibiting nonspecific control, besides, they caused increased activity of enzymes related to pathogenesis and induction of resistance (Ludwig and Moura, 2009). …”

Section: Figure 1 Relative Percentage Of Gerlachia Oryzae Andmentioning

confidence: 99%

Biocontrol and seed transmission of Bipolaris oryzae and Gerlachia oryzae to rice seedlings

et al. 2014

Self Cite

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-Bipolaris oryzae and Gerlachia oryzae, which cause rice brown spot and leaf scald, respectively, are mainly disseminated by seeds. The aim of this study was to evaluate the potential of seeds microbiolization to reduce transmission of these pathogens to seedlings by using the bacteria DFs185 (Pseudomonas synxantha), DFs223 (P. fluorescens), DFs306 (unidentified) and DFs418 (Bacillus sp.). Seeds naturally infested/infected with both pathogens were immersed in suspension of these bacteria (A 540 = 0.5) individually or in saline solution (control treatment). After 30 minutes of agitation at 10 °C, 400 seeds were submitted to a sanity test through the blotter method and the isolate DFs223 was the best to reduce the incidence of B. oryzae and G. oryzae in both seed lots evaluated. Seeds treated like above were sowed in sterilized vermiculite. Seed transmission and growth promotion were recorded after 21 days of incubation in the same conditions. The isolates DFs185 and DFs306 reduced transmission of both pathogens, although the isolate DFs306 was the one wich gave the greatest growth increases. The evaluation of the in vitro antibiosis showed that isolates inhibited the mycelial growth of both pathogens, except DFs306. It is possible to affirm that these bacteria have the potential to be used as a seed treatment for seed-borne disease control.Index terms: biological control, rice brown spot, rice leaf scald, seed treatment, Oryza sativa L. Biocontrole e transmissão de Bipolaris oryzae eGerlachia oryzae para plântulas de arroz RESUMO -Bipolaris oryzae e Gerlachia oryzae causadores, respectivamente, da mancha parda e da escaldadura do arroz são principalmente disseminados por sementes. Objetivou-se com esse trabalho avaliar o potencial da microbiolização de sementes para a redução da transmissão destes patógenos das sementes para as plântulas usando as bactérias DFs185 (Pseudomonas synxantha), DFs223 (P. fluorescens), DFs306 (não identificado) e DFs418 (Bacillus sp. Termos para indexação: controle biológico, mancha parda do arroz, escaldadura do arroz, tratamento de sementes, Oryza sativa.

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“…In the work performed by Ludwig et al (2013), mortality increase of second stage juveniles (J2) of Meloidogyne javanica in all bacterial suspensions tested when the exposure time increased from 24 to 48 h was detected. Similar results were observed in this study when the expansion of the exposure time of juveniles and adults of R. similis to bacterial metabolites caused an increase in the percentage of dead nematodes.…”

Section: Discussionmentioning

confidence: 96%

“…Recent surveys report that the bacteria Pseudomonas synxantha, Pseudomonas fluorescens, Bacillus subtilis, and Stenotrophomonas maltophilia showed toxic effect on eggs and juveniles under laboratory conditions indicating the occurrence of the production of antimicrobial metabolites (Ludwig et al, 2013). Chaves et al (2009) selecting microorganisms for the biocontrol of R. similis, reported the occurrence of biocontrol percentage of 92% provided by Bacillus and significant increases in biomass production in banana plantlets of the 'Grand Naine' cultivar.…”

Section: Introductionmentioning

confidence: 99%

An evaluation in vitro and in vivo of rhizobacteria in the biocontrol of Radopholus similis in banana

Celma¹,

Aldo²,

Harllen³

et al. 2015

Afr. J. Agric. Res.

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Rhizobacteria were isolated from the rhizosphere of banana cultivars 'Prata Comum' and 'Prata Anã' and from crotalaria and marigold in Tryptic Soy Agar and King B-agar media. Bacteria were used for valuations of the in vitro and in vivo. The promising isolates were submitted to identification by polymerase chain reaction. Among the one hundred and fifty two evaluated, twenty three showed toxicity to R. similis after twenty-four and thirty six hours. Five presented deleterious effect on the population of R. similis in the soil under in vivo conditions. Of the ten submitted to identification, three corresponded to the Bacillus genus and two to the Pseudomonas genus. The bacteria evaluated have antagonistic potential against R. similis.

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Potencial da microbiolização de sementes de arroz com rizobactérias para o biocontrole do nematoide das galhas

Cited by 22 publications

References 20 publications

ISOLATION AND CHARACTERIZATION OF PLANT GROWTH PROMOTING BACTERIA ISOLATED FROM GARLIC (allium sativum).

ISOLATION AND CHARACTERIZATION OF PLANT GROWTH PROMOTING BACTERIA ISOLATED FROM GARLIC (allium sativum).

Biocontrol and seed transmission of Bipolaris oryzae and Gerlachia oryzae to rice seedlings

An evaluation in vitro and in vivo of rhizobacteria in the biocontrol of Radopholus similis in banana

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