Mass spore production and inoculation of Calonectria pteridis on Eucalyptus spp. under different environmental conditions

Alfenas, Rafael Ferreira; Pereira, Olinto Liparini; Freitas, Rodrigo G.; Freitas, Camila S.; Dita, Miguel; Alfenas, Acelino C.

doi:10.1590/s1982-56762013000500005

Cited by 25 publications

(29 citation statements)

References 12 publications

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“…This is regarded as one of the most prominent species complexes associated with CLB on Eucalyptus in that country (Alfenas et al., 2004, Alfenas et al., 2013c, Alfenas et al., 2015, Graça et al., 2009). Calonectria amazonica , C. amazoniensis , C. lageniformis , C. tereticornis and C. tropicalis were all isolated from CLB leaf lesions on Eucalyptus spp.…”

Section: Discussionmentioning

confidence: 99%

The forgotten Calonectria collection: Pouring old wine into new bags

Lombard

Wingfield

Alfenas

et al. 2016

Studies in Mycology

110

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The genus Calonectria with its Cylindrocladium asexual morphs has been subject to several taxonomic revisions in the past. These have resulted in the recognition of 116 species, of which all but two species (C. hederae and C. pyrochroa) are supported by ex-type cultures and supplemented with DNA barcodes. The present study is based on a large collection of unidentified Calonectria isolates that have been collected over a period of 20 years from various substrates worldwide, which has remained unstudied in the basement of the CBS-KNAW Fungal Biodiversity Centre. Employing a polyphasic approach, the identities of these isolates were resolved and shown to represent many new phylogenetic species. Of these, 24 are newly described, while C. uniseptata is reinstated at species level. We now recognise 141 species that include some of the most important plant pathogens globally.

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Section: Discussionmentioning

confidence: 99%

The forgotten Calonectria collection: Pouring old wine into new bags

Lombard

Wingfield

Alfenas

et al. 2016

Studies in Mycology

110

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“…For calonectria leaf blight disease of eucalypt, shorter incubation period and higher leaf area affected were observed when different eucalypt clones were inoculated with Calonectria pteridis and kept in the dark when compared with clones kept under continuous light (Alfenas et al. ).…”

Section: Discussionmentioning

confidence: 99%

Infection Process of Botrytis cinerea on Eucalypt Leaves

Caires

Rodrigues

Furtado

2014

Journal of Phytopathology

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This study aimed to elucidate the infection process of Botrytis cinerea on eucalypt leaves. Tests were conducted to evaluate the influence of leaf side (adaxial or abaxial), leaf age and luminosity on conidial germination, appressorium formation and grey mould (GM) severity. The adaxial and abaxial surfaces of detached eucalypt leaves were inoculated with a conidial suspension of B. cinerea and kept under constant light or dark. Subsequently, the adaxial surface of young and old leaves was inoculated and kept in the dark. To evaluate the percentage of conidia germination and appressorium formation, leaf samples were collected 6 hours after inoculation (hai), clarified (alcohol and chloral hydrate) and evaluated under a light microscope. The severity of GM was assessed 10 days after inoculation. For scanning electron microscopy analysis, samples were collected from 2 to 168 hai. A higher percentage of conidia germination (92%) and GM severity (21%) occurred on the adaxial surfaces of leaves kept in the dark. There was no statistical difference between the surfaces of young and old leaves for conidia germination. No appressorium was formed by B. cinerea. The GM severity on young leaves (17.3%) was 34 times higher than on old leaves (0.5%). The micrographs showed germinating conidia emitting 1–4 germ tubes in samples at 4 hai. The fungus penetration occurred through intact leaf surfaces, and both extra‐ and intracellular colonization of the mesophyll cells by the hyphae of the pathogen were observed at 120 hai. Sporulation occurred on the adaxial and abaxial surfaces (macronematous conidiophores) and below the epidermis (micronematous conidiophores).

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“…After this period, the fungal cultures were submitted to physical stress to stimulate the production of conidia according to Alfenas et al (2013). After this period, the fungal cultures were submitted to physical stress to stimulate the production of conidia according to Alfenas et al (2013).…”

Section: Inoculum Preparationmentioning

confidence: 99%

“…To collect the conidia, sterile water containing 0.05% Tween 20 was added to the plates, and then, the surface of the colony was scraped off with the aid of a sterile brush (Alfenas et al, 2013). To collect the conidia, sterile water containing 0.05% Tween 20 was added to the plates, and then, the surface of the colony was scraped off with the aid of a sterile brush (Alfenas et al, 2013).…”

Section: Inoculum Preparationmentioning

confidence: 99%

Phosphorus, nitrogen and potassium nutrition on Calonectria leaf blight in eucalypt plants

et al. 2019

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Calonectria pteridis causes Calonectria leaf blight (CLB), and consequently severe defoliation in eucalypt plantations, which results in losses in wood volume. To reduce the negative impacts of this disease in eucalypt, this study aimed to assess the application of different doses and combinations of the macronutrients N, P and K on the percentage of symptomatic leaf area (SLA) and defoliation induced by the pathogen. Cuttings of a clone of Eucalyptus grandis were transplanted to pots containing soil that received different dose combinations of N, P and K, according to an incomplete factorial design. At 200 days after transplanting, leaf samples were analysed for N, P and K contents, and then, the plants were inoculated. Forty‐five days post‐inoculation, SLA and percentage of defoliation were quantified. Potassium doses above 75 mg/dm3 of soil significantly reduced SLA and defoliation. The influence of N and P on defoliation was dependent on K doses, but both reduced symptomatic leaf area. The best control of the disease, expressed by decreased defoliation and symptomatic leaf area, was achieved with leaf content of N, P and K of 9.8, 0.8 and 10.4 g per kg leaf, respectively, obtained with doses of 55, 82.5 and 143 mg/dm3of soil, respectively. Therefore, N, P and K nutrition can be a component of an integrated management programme for the control of CLB in eucalypts.

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Mass spore production and inoculation of Calonectria pteridis on Eucalyptus spp. under different environmental conditions

Cited by 25 publications

References 12 publications

The forgotten Calonectria collection: Pouring old wine into new bags

The forgotten Calonectria collection: Pouring old wine into new bags

Infection Process of Botrytis cinerea on Eucalypt Leaves

Phosphorus, nitrogen and potassium nutrition on Calonectria leaf blight in eucalypt plants

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