Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV)

Aguilera, Jorge González; Tavares, Sheila S.; Sobrinho, Roberto Ramos; Xavier, César Augusto Diniz; Dueñas-Hurtado, Francisco; Lara-Rodrigues, Regla M.; Silva, Derly J. H. da; Zerbini, F. Murilo

doi:10.1590/s1982-56762012000500007

Cited by 15 publications

(10 citation statements)

References 33 publications

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“…Previous studies have shown that the high genetic variability observed in some begomovirus populations are directly related to the frequent occurrence of recombination events, and that recombination sites are not randomly distributed along the begomovirus genome, with breakpoints being more frequently found in the CR and the Rep gene (Stanley 1995;Fauquet et al 2005;García-Andrés et al 2007;Lefeuvre et al 2007;Ribeiro et al 2007;Martin et al 2011;Silva et al 2012;Lima et al 2013;Rocha et al 2013;Ramos-Sobrinho et al 2014; reviewed by Lefeuvre and Moriones 2015). Here, the ToMoLCV Rep gene was found to be much more variable than previously published results for tomato-infecting begomoviruses (González-Aguilera et al 2012;Rocha et al 2013). The Rep gene was six times more diverse than the To M o L C V C P g e n e i n t h e P B s u b p o p u l a t i o n .…”

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confidence: 42%

“…c Numbering starts at the first nucleotide after the cleavage site at the origin of replication and increases clockwise populations (Navas-Castillo et al 2011;González-Aguilera et al 2012;Lima et al 2013;Rocha et al 2013;Ramos-Sobrinho et al 2014). ToMoLCV and SiMoAV subpopulations showed high genetic variability, with the ToMoLCV Rep gene being the most variable (Table 3).…”

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confidence: 99%

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Genetic structure of populations of the begomoviruses Tomato mottle leaf curl virus and Sida mottle Alagoas virus infecting tomato (Solanum lycopersicum) and Sida spp., respectively

Ferro

Ramos-Sobrinho

Silva

et al. 2016

Trop. plant pathol.

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Begomoviruses are among the most damaging pathogens infecting cultivated hosts worldwide, and are considered an important limiting factor in tomato fields. In the present study, foliar samples of tomato and Sida spp. were collected in Boqueirão, state of Paraíba, Brazil, in May 2014. Viral genomes were amplified, cloned and sequenced. A total of 30 DNA-A components were obtained. Pairwise comparisons indicated the presence of only two begomovirus species: Tomato mottle leaf curl virus (ToMoLCV) from tomato samples and Sida mottle Alagoas virus (SiMoAV) in Sida spp. ToMoLCV and SiMoAV subpopulations showed high genetic variability, with the ToMoLCV Rep gene being the most variable. Recombination events were detected only among ToMoLCV isolates, with recombination breakpoints occurring in the common region and in the Rep gene. Negative or purifying selection seems to be among the most important evolutionary mechanisms acting on the CP and Rep genes in both ToMoLCV and SiMoAV. Our results indicate that ToMoLCV remains as the main tomato-infecting begomovirus in northeastern Brazil, and that Sida spp. do not contribute to ToMoLCV epidemics in tomato. Keywords Geminiviridae. ssDNA virus. Evolutionary mechanisms The family Geminiviridae comprises circular ssDNA plant viruses encapsidated into quasi-icosahedral geminate particles, and is divided into seven genera (Becurtovirus, Begomovirus, Curtovirus, Eragrovirus, Mastrevirus, Topocuvirus and Turncurtovirus) according to the type of insect vector, host range, genome organization and phylogeny (Varsani et al. 2014). Viruses belonging to the genus Begomovirus have one (monopartite) or two (bipartite) genomic components known as DNA-A and DNA-B, and are transmitted by the whiteflies of the Bemisia tabaci sibling species group (Homoptera: Aleyrodidae) to dicotyledonous plants (Brown et al. 2012). Begomoviruses are economically important pathogens infecting cultivated plants worldwide (Legg and Fauquet 2004; Morales and Anderson 2001; Navas-Castillo et al. 2011; Varma and Malathi 2003), and are considered limiting factors to production of tomatoes, peppers and legumes in the New World (Morales 2006; Navas-Castillo et al. 2011). The emergence of begomoviruses as important pathogens in tomato-growing areas in Brazil coincided with the introduction and spread of B. tabaci Middle East-Asia Minor 1 (MEAM1, previously known as biotype B) in the early 1990's (

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confidence: 42%

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confidence: 99%

Genetic structure of populations of the begomoviruses Tomato mottle leaf curl virus and Sida mottle Alagoas virus infecting tomato (Solanum lycopersicum) and Sida spp., respectively

Ferro

Ramos-Sobrinho

Silva

et al. 2016

Trop. plant pathol.

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“…; Gonzáles‐Aguilera et al. ). In Sumaré, São Paulo state, an important region for the fresh market tomato production, ToSRV has displaced Tomato yellow vein streak virus (ToYVSV), which was the most prevalent begomovirus species a few years ago (Della Vecchia et al.…”

Section: Discussionmentioning

confidence: 99%

Temporal Dynamics of Tomato Severe Rugose Virus and Bemisia tabaci in Tomato Fields in São Paulo, Brazil

Barbosa

Rezende

Amorim

et al. 2015

Journal of Phytopathology

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Temporal progress of a begomovirus disease in tomato fields and the abundance of its whitefly vector, Bemisia tabaci biotype B, were evaluated during three consecutive tomato plantings in the municipality of Sumaré, state of São Paulo, Brazil, in 2006 and 2007. The incidence of symptomatic plants and the number of adult whiteflies were weekly monitored on experimental plots randomly chosen in tomato commercial fields. Tomato severe rugose virus (ToSRV) was identified as the causal agent of the disease, and its relationships with other Brazilian begomoviruses was confirmed by partial and complete nucleotide sequencing of the viral genome. The disease temporal progress was analysed by fitting different models to disease incidence. The monomolecular model showed the best fit, which is consistent with a predominant role of primary spread in the epidemiology of ToSRV. A higher number of adult whiteflies were observed at the borders of the plots, also suggesting primary spread of ToSRV from external sources of inoculum, which might be represented by weeds and volunteer tomato‐infected plants. In Brazil, since 2004, there is a legislative measure that mandates, for some regions of processing tomato plantings, a 2‐month crop‐free period during the year. Based on our results, we suggest the extension of this measure to all tomato‐producing regions, including fresh market tomato. We also suggest that growers emphasize the elimination of old plants from harvested fields that can serve as virus reservoirs several weeks prior to new plantings and weeds nearby the fields to limit the primary spread of ToSRV.

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“…The species diversity of begomoviruses has been surveyed extensively (Ala-Poikela et al , 2005; Albuquerque et al , 2012; Bull et al , 2006; Castillo-Urquiza et al , 2008; Fernandes et al , 2009; García-Andrés et al , 2006; Lozano et al , 2009; Ndunguru et al , 2005; Reddy et al , 2005; Ribeiro et al , 2003; Rothenstein et al , 2006; Sserubombwe et al , 2008; Tavares et al , 2012), and some studies have investigated the genetic structure of begomovirus populations in cultivated and non-cultivated hosts in different geographical regions (Ge et al , 2007; González-Aguilera et al , 2012; Lima et al , 2013; Rocha et al , 2013; Silva et al , 2012, 2011). Strikingly, in spite of its great economic importance as the most damaging viral pathogen of bean crops in South America (Morales, 2006), no studies on the genetic variability of BGMV have been conducted since Faria & Maxwell (1999) analysed 20 partial DNA-A sequences.…”

Section: Discussionmentioning

confidence: 99%

Contrasting genetic structure between two begomoviruses infecting the same leguminous hosts

Sobrinho

Xavier

Pereira

et al. 2014

Journal of General Virology

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Begomoviruses are whitefly-transmitted, ssDNA plant viruses and are among the most damaging pathogens causing epidemics in economically important crops worldwide. Wild/non-cultivated plants play a crucial epidemiological role, acting as begomovirus reservoirs and as 'mixing vessels' where recombination can occur. Previous work suggests a higher degree of genetic variability in begomovirus populations from non-cultivated hosts compared with cultivated hosts. To assess this supposed host effect on the genetic variability of begomovirus populations, cultivated (common bean, Phaseolus vulgaris, and lima bean, Phaseolus lunatus) and non-cultivated (Macroptilium lathyroides) legume hosts were sampled from two regions of Brazil. A total of 212 full-length DNA-A genome segments were sequenced from samples collected between 2005 and 2012, and populations of the begomoviruses Bean golden mosaic virus (BGMV) and Macroptilium yellow spot virus (MaYSV) were obtained. We found, for each begomovirus species, similar genetic variation between populations infecting cultivated and non-cultivated hosts, indicating that the presumed genetic variability of the host did not a priori affect viral variability. We observed a higher degree of genetic variation in isolates from MaYSV populations than BGMV populations, which was explained by numerous recombination events in MaYSV. MaYSV and BGMV showed distinct distributions of genetic variation, with the BGMV population (but not MaYSV) being structured by both host and geography.

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Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV)

Cited by 15 publications

References 33 publications

Genetic structure of populations of the begomoviruses Tomato mottle leaf curl virus and Sida mottle Alagoas virus infecting tomato (Solanum lycopersicum) and Sida spp., respectively

Genetic structure of populations of the begomoviruses Tomato mottle leaf curl virus and Sida mottle Alagoas virus infecting tomato (Solanum lycopersicum) and Sida spp., respectively

Temporal Dynamics of Tomato Severe Rugose Virus and Bemisia tabaci in Tomato Fields in São Paulo, Brazil

Contrasting genetic structure between two begomoviruses infecting the same leguminous hosts

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